Plant Protect. Sci., 2025, 61(3):222-241 | DOI: 10.17221/127/2024-PPS

Mitigation of salinity stress effects on Vicia faba L. growth and productivity using proline and salicylic acid foliar applicationOriginal Paper

Hossam El-Beltagi ORCID...1,2, Mohamed El-Nady3, Ahmed Mahmoud Ismail4,5,6, Metwaly Mahfouz Salem Metwaly3
1 Agricultural Biotechnology Department, College of Agriculture and Food Sciences, King Faisal University, Al-Ahsa, Saudi Arabia
2 Biochemistry Department, Faculty of Agriculture, Cairo University, Giza, Egypt
3 Department of Agricultural Botany, Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh, Egypt
4 Department of Arid Land Agriculture, College of Agriculture and Food Sciences, King Faisal University, Al-Ahsa, Saudi Arabia
5 Pests and Plant Diseases Unit, College of Agriculture and Food Sciences, King Faisal University, Al-Ahsa, Saudi Arabia
6 Vegetable Diseases Research Department, Plant Pathology Research Institute, Agricultural Research Center (ARC), Giza, Egypt

High soil salinity causes a negative impact on plant growth and lowers crop yields. Thus, pot experiments were conducted to investigate the impact of foliar application of salicylic acid (SA) and proline (Pro), separately and combined, on enhancing salinity tolerance in broad beans. Salinity stress (4.69 ds/m and 6.25 ds/m) significantly reduced plant growth (plant height, leaf area, number of leaf/plant, plant dry weight), chlorophyll pigment content (chlorophyll a, b or total), relative water content, K/Na ratio, seed yield per plant, and N, P, K, and crude protein content in broad bean seeds. Foliar application of Pro and SA, either individually or in combination, enhanced plant growth parameters, chlorophyll pigment content, endogenous proline levels, phenol content, and the activities of antioxidant enzymes [antioxidant enzymes including catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD)]. Additionally, these treatments enhanced plant seed yield, N, P, K, and crude protein levels in the seeds. The combined foliar application of Pro and SA was more effective in mitigating salinity stress’s harmful effects than using either substance alone. These findings indicate that foliar application of SA and Pro, either individually or in combination, alleviated the adverse effects of salinity on broad beans, with the combined application proving to be the most effective.

Keywords: chlorophyll pigments; endogenous proline content; antioxidant enzymes activity; relative water content; phenols content; membrane stability

Received: July 28, 2024; Revised: March 8, 2025; Accepted: April 17, 2025; Prepublished online: May 4, 2025; Published: July 20, 2025  Show citation

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El-Beltagi H, El-Nady M, Ismail AM, Metwaly MMS. Mitigation of salinity stress effects on Vicia faba L. growth and productivity using proline and salicylic acid foliar application. Plant Protect. Sci. 2025;61(3):222-241. doi: 10.17221/127/2024-PPS.
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