Plant Protect. Sci., 2022, 58(1):57-64 | DOI: 10.17221/6/2021-PPS

An investigation of the genotoxic and cytotoxic effects of myclobutanil fungicide on plantsOriginal Paper

Ismail Poyraz ORCID...*,1,2
1 Molecular Biology and Genetics Department, Faculty of Science and Letters, Bilecik Seyh Edebali University, Bilecik, Turkey
2 Biotechnology Application and Research Centre, Molecular Biology and Genetics Department, Bilecik Seyh Edebali University, Bilecik, Turkey

Myclobutanil is a chemical pesticide commonly used in the production of some vegetables and fruits like greenhouse peppers, grapes, and apples. The aim of this study was to investigate the genotoxic and cytotoxic effects of myclobutanil fungicide on the Allium cepa plant, the model organism. Randomly amplified polymorphic DNA and inter simple sequence repeat-PCR techniques were performed on the DNA of A. cepa exposed to the different myclobutanil doses and time periods. The nucleus anomalies and abnormal anaphases were investigated using a light microscope. PCR analyses showed that myclobutanil causes some DNA sequence changes on the onion genome depending on the increase in the fungicide dose and exposure time. It was determined that myclobutanil has a serious genotoxic effect, even in low doses like 25-50 ppm.

Keywords: pesticides; toxicity; DNA-markers; Allium cepa

Published: December 17, 2021  Show citation

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Poyraz I. An investigation of the genotoxic and cytotoxic effects of myclobutanil fungicide on plants. Plant Protect. Sci. 2022;58(1):57-64. doi: 10.17221/6/2021-PPS.
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