Plant Protect. Sci., 1999, 35(3):79-84 | DOI: 10.17221/9703-PPS

Serological and biochemical distinguishing of Pseudomonas syringae pathovars on peasOriginal Paper

Iveta Pánková, Blanka Kokošková

Polyclonal antisera to detect and determine two related pathovars, Pseudomonas syringae pv. pisi and Pseudomonas syringae pv. syringae, were prepared Untreated bacterial cells, or fixed by formaldehyde or glutaraldehyde were used as antigens. After cross-absorption with heterologous antigens, antisera revealed a high level of specificity in slide agglutination, Ouchterlony gel double-diffusion and in DAS-ELISA and PTA-ELISA. Each of 14 P s. pisi prepared polyclonal antisera could detect and deter­ mine all strains of P s. pisi, regardless of race. PTA-ELISA was the most appropriate serological test to distinguish Pseudomonas syringae pathovars on peas. Most of the P s. pisi strains from foreign collections were in serological tests confirmed asP. s. pisi, while most of the Czech strains suspected as P s. pisi were determined as P s. syringae strains. The principal biochemical reaction, i.e., use of DL-homoserine as a carbon source to grow P s. pisi but not P s. syringae , was proved not to be sufficiently reliable to distinguish both pathovars.

Keywords: bacterial blight of peas; Pseudomonas syringae pv. pisi; Pseudomonas syringae pv. syringae: slide agglutination; Ouchterlony gel double-diffusion; ELISA

Published: September 30, 1999  Show citation