Plant Protect. Sci., 2002, 38(10):S108-S111 | DOI: 10.17221/10331-PPS

Identification and characterization of host proteins interacting with NSm, the Tomato spotted wilt virus movement proteinOriginal Paper

M. Paape, S. Nell, S. Von Bargen, J.-W. Kellmann
Universität Rostock, Fachbereich Biowissenschaften, Abteilung Biochemie, D-18059 Rostock, Germany

To search for host proteins involved in systemic spreading of Tomato spotted wilt virus (TSWV), the virus-encoded NSm movement protein has been utilized as a bait in yeast two-hybrid interaction trap assays. J-domain chaperones from different host species and a protein denominated At-4/1 from Arabidopsis thaliana showing homologies to myosins and kinesins were identified as NSm-interacting partners. In this communication we illustrate that following TSWV infection, J-domain proteins accumulated in systemically infected leaves of A. thaliana, whereas At-4/1 was constitutively detected in leaves of A. thaliana and Nicotiana rustica.

Keywords: Tomato spotted wilt virus (TSWV); Arabidopsis thaliana; Nicotiana rustica; movement protein; J-domain protein; yeast two-hybrid system

Published: June 30, 2002  Show citation

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Paape M, Nell S, Von Bargen S, Kellmann J-W. Identification and characterization of host proteins interacting with NSm, the Tomato spotted wilt virus movement protein. Plant Protect. Sci. 2002;38(SI 1 - 6th Conf EFPP):S108-111. doi: 10.17221/10331-PPS.
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