Plant Protect. Sci., 2002, 38(10):S117-S121 | DOI: 10.17221/10333-PPS

Origin of resistance to Plum pox virus in apricot: microsatellite (Ssr) data analysisOriginal Paper

T.N. Zhebentyayeva, G.L. Reighard, B. Kr¹ka, V.M. Gorina, A.G. Abbott
1 Department of Horticulture, Clemson University, Clemson SC, 29634 USA
2 Department of Genetics and Biochemistry, Clemson University, Clemson SC, 29634 USA
3 Faculty of Horticulture, Mendel University of Agriculture and Forestry in Brno, 691 44 Lednice na Moravì, Czech Republic; 4 Department of Horticulture, Nikita Botanical Garden, Yalta, Crimea, 98648 Ukraine Tel.: +864 656 9595, Fax: +864 656 4960, E-mail: tzhebe@clemson.edu

The objective of this study was to establish the genetic relationship among cultivars commonly used as donors for resistance to Plum pox virus (PPV) in order to identify the putative sources of resistance to PPV. The plant material tested represented the European, Central Asian and Chinese ecogeographical groups of cultivated apricots as well as the wild Dzhungar-Zailij population of Prunus armeniaca L. Forty-eight native accessions as well as the resistant (or tolerant) cultivars Harlayne, Stark Early Orange (SEO), Goldrich, Vestar and two hybrid forms Vestar × SEO (LE 3276) and Velkopavlovická × SEO (LE 2904) were screened by means of SSR analysis. To elucidate genetic relationships among apricot germplasm, a dendrogram was produced using neighbor joining (NJ) analysis of Nei's pair-wise genetic distances over 14 polymorphic SSR markers. On the dendrogram, resistant cultivars were separated into two different clusters suggesting two different sources of resistance to PPV. As was expected from pedigrees, SEO, Vestar, LE 2904 and LE 3276 were grouped together in a cluster adjacent to the European cultivars. Resistant cultivars Harlayne and Goldrich were within another group containing Central Asian apricots and Dzhungar-Zailij form.

Keywords: sharka; Prunus armeniaca; microsatellite markers; genetic similarity

Published: June 30, 2002  Show citation

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Zhebentyayeva TN, Reighard GL, Kr¹ka B, Gorina VM, Abbott AG. Origin of resistance to Plum pox virus in apricot: microsatellite (Ssr) data analysis. Plant Protect. Sci. 2002;38(SI 1 - 6th Conf EFPP):S117-121. doi: 10.17221/10333-PPS.
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    References

    1. ATANASSOV D. (1932): Plum pox. A new virus disease. Ann. Univ. Sofia. Fac. Agric. Silvic., 11: 49-69.
    2. AUDERGON J.M., DOSBA F., KARAYIANNIS I., DICENTA F. (1994): Amélioration de l'abricotier pour la résistance à la sharka. EPPO Bull., 24: 741-748. Go to original source...
    3. BADENES M.L., ASÍNS M.J., CARBONELL E.A., GLÁCER G. (1996): Genetic diversity in apricot, Prunus armeniaca, aimed at improving resistance to Plum pox virus. Plant Breeding, 115: 133-139. Go to original source...
    4. BASSI D., BELLINI E., GUERRIERO R., MONASTRA F., PENNONE F. (1995): Apricot breeding in Italy. Acta Horticult., 384: 47-54. Go to original source...
    5. BROOKS R.M., OLMO H.P. (1997): The Brooks and Olmo Register of Fruit and Nut Varieties, 3rd Ed. ASHS Press, Alexandria, Virginia, USA.
    6. DE VICENTE M.C., TRUCO M.J., EGEA J., BURGOS L., ARÚS P. (1998): RFLP variability in apricot (Prunus armeniaca L.). Plant Breeding, 117: 153-158. Go to original source...
    7. E GEA J., BURGOS L., MARTÍNEZ-GÓMEZ P., DICENTA F. (1999): Apricot breeding for sharka resistance at the CEBAS-CSIC Murcia (Spain). Acta Hortic., 488: 153-157. Go to original source...
    8. FAUST M., SURÁNYI D., NYUJTÓ F. (1998): Origin and dissemination of apricot. Hortic. Rev., 22: 225-266. Go to original source...
    9. FELSENSTEIN J. (1989): PHYLIP - Phylogeny Interference Package. Cladistics, 5: 164-166.
    10. HORMAZA J.I. (2002): Molecular characterization and similarity relationships among apricot (Prunus armeniaca L.) genotypes using simple sequence repeats. Theor. Appl. Genet., 104: 321-328. Go to original source... Go to PubMed...
    11. HURTADO M.A., WESTMAN A., BECK E., ABBOTT A.G., LLÁCER G., BADENES M.L. (2002): Genetic diversity in apricot cultivars involved in breeding for sharka resistance based on AFLP markers. (in press)
    12. KARAYIANNIS I., MAINOU A., SYRGYANNIDIS G.D., STYLIANNIDIS D.C. (1991): Apricot cultivars considered as resistant to plum pox virus in Greece and used as genitor in crossings. Deuxièmes Rencontres Agrimed sur l'Abricotier. EUR 15009 FR. Commission des Communautés Européennes, Luxembourg: 129-133.
    13. KOSTINA K.F. (1964): Application the phytogeographical method to apricot classification. Trud. Nikit. Bot. Gard., 24. Kolos, Moscow. (in Russian)
    14. LAYNE R.E.C., BAILEY C.H., HOUGH L.F. (1996): Apricots. In: JANICK J., MOORE J.N. (eds): Fruit Breeding, Vol. 1. Tree and Tropical Fruits. John Wiley & Sons, Inc, New York: 79-111.
    15. MINCH E. (1997): MICROSAT, Version 1.5 d. Stanford University Medical Center, Stanford, CA.
    16. PAGE R.D.M. (1996): TREEWIEV: an application to display phylogenetic trees on personal computers. Comp. Appl. Biosci., 12: 357-358. Go to original source... Go to PubMed...
    17. POLÁK J. (1994): Breeding for resistance to plum pox potyvirus in the Czech Republic. EPPO Bull., 24: 781-782. Go to original source...
    18. POLÁK J., OUKROPEC I., KOMÍNEK P., KR©KA B., BITTÓOVÁ M. (1997): Detection and evaluation of resistance of apricots and peaches to plum pox virus. J. Plant Dis. Protect., 104: 466-473.
    19. R OY A.S., S MITH I.M. (1994): Plum pox situation in Europe. EPPO Bull., 24: 515-523. Go to original source...
    20. SIVOLAP YU.M. (ed.) (1998): Application of PCR analysis to genetic research and breeding. Agrarna Nauka, Kiev. (in Russian)
    21. WANG Y., GEORGI L.L., ZHEBENTYAYEVA T.N., REIGHARD G.L., SCORZA R., ABBOTT A.G. (2002): High-throughput targeted SSR marker development in peach (Prunus persica). Genome, 45: 319-328. Go to original source... Go to PubMed...
    22. ZHEBENTYAYEVA T.N., REIGHARD G.L., GORINA V.M., ABBOTT A.G. (2002): Simple sequence repeat (SSR) analysis for assessment of genetic variability in apricot germplasm. Theor. Appl. Genet. (in press) Go to original source... Go to PubMed...

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