Plant Protect. Sci., 2002, 38(10):S21-S23 | DOI: 10.17221/10312-PPS

Development of RT-PCR tests for the routine detection of latent and ILAR viruses in fruit treesOriginal Paper

S. Marbot, J. Kummert, M. Salmon, M. Vendrame, A. Huwaert, O. Dutrecq, P. Lepoivre
Plant Pathology Unit, Agricultural University of Gembloux (FUSAGx), 5030 Gembloux, Belgium * Tel.: +32 81 622 431, Fax: +32 81 610 126, E-mail: roussel.s@fsagx.ac.be

The detection throughout the year of latent and ILAR viruses in fruit trees by classical serological tests appears to be unreliable. Recently, these problems have smoothed themselves out by the use of molecular methods. We have developed RT-PCR protocols which are simple and reliable for routine detection of these viruses throughout the year.

Keywords: ACLSV; ASPV; ASGV; PDV; PNRSV; ApMV; fruit trees; RT-PCR; RT-PCR-ELOSA; Minor Groove Binder-DNA probe

Published: June 30, 2002  Show citation

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Marbot S, Kummert J, Salmon M, Vendrame M, Huwaert A, Dutrecq O, Lepoivre P. Development of RT-PCR tests for the routine detection of latent and ILAR viruses in fruit trees. Plant Protect. Sci. 2002;38(SI 1 - 6th Conf EFPP):S21-23. doi: 10.17221/10312-PPS.
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    References

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