Plant Protect. Sci., 2002, 38(10):S31-S34 | DOI: 10.17221/10316-PPS

Discrimination of Czech Armillaria species based on PCR method and high performance liquid chromatographyOriginal Paper

J. Lochman, O. Šerý, L. Jankovský, V. Mikes
1 Department of Biochemistry and
2 Department of Comparative Animal Physiology and General Zoology, Faculty of Science, Masaryk University, 611 37 Brno
3 Department of Forest Protection, Faculty of Forestry, Mendel University of Agriculture and Forestry, 613 00 Brno, Czech Republic Tel.: +420 541 129 410, Fax: +420 541 129 623, E-mail: mikes@chemi.muni.cz

The genus Armillaria belongs to basidiomycetes and has been known to induce root rot disease and to cause extensive economic losses to a forest crop. We analysed about 40 isolates of Armillaria collected in Czech Republic by PCR and restriction analysis using gel electrophoresis and ion-exchange HPLC. Restrictase Hinf I was able to discriminate all investigated Armillaria species. The sensitivity and resolution of HPLC method was better than that performed by gel electrophoresis. HPLC was able to detect some heterozygous. The results prove the similarity of the species A. borealis, A. cepistipes, A. gallica, A. ostoyae in difference of A. mellea and A. tabescens.

Keywords: Armillaria; PCR; forest pathogen; basidiomycete; root rot disease

Published: June 30, 2002  Show citation

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Lochman J, Šerý O, Jankovský L, Mikes V. Discrimination of Czech Armillaria species based on PCR method and high performance liquid chromatography. Plant Protect. Sci. 2002;38(SI 1 - 6th Conf EFPP):S31-34. doi: 10.17221/10316-PPS.
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    References

    1. CHILLALI M., IDDER-IGHILI H., GUILLAUMIN J.J., MOHAMMED C. (1998a): Variation in the ITS and IGS regions of ribosomal DNA among the biological species of European Armillaria. Mycol. Res., 102: 533-540. Go to original source...
    2. CHILLALI M., WIPF D., GUILLAUMIN J.J., MOHAMMED C., BOTTON B. (1998b): Delineation of the European Armillaria species based on the sequences of the internal transcribed spacer (ITS) ofribosomal DNA. New Phytol., 138: 553-561. Go to original source...
    3. K ATZ E.D., D ONG M.W. (1990): Rapid analysis and purification of polymerase chain reaction products by high-performance liquid chromatography. BioTechniques, 8: 546-554. Go to PubMed...
    4. SCHMIDT O. (2001): NCIB database. AJ 250051-55.
    5. WHITE T.J., BRUNS T., LEE S., TAYLOR J. (1990): Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols: A Guide to Methods and Applications, Acad. Press, Inc.: 315-322 Go to original source...

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