Plant Protect. Sci., 2002, 38(10):S43-S48 | DOI: 10.17221/10318-PPS

Investigation of differences between wheat and barley forms of Wheat dwarf virus and their distribution in host plantsOriginal Paper

J. Schubert, A. HABEKUß, F. Rabenstein

¹ Federal Centre for Breeding Research on Cultivated Plants - Institute of Resistance Research and Pathogen Diagnostics and

² Institute of Epidemiology and Resistance, Aschersleben, 06449 Aschersleben, Germany Tel.: +49 3473 879 166, Fax: +49 3473 879 200, E-mail: j.schubert@bafz.de

Wheat dwarf virus, a monogemini virus, infects several cereal species. Until now complete sequence data have been published only for wheat isolates. We cloned the complete DNA of 21 isolates from wheat, barley and Lolium spec. and compared the sequences with published data. Two types of the virus were found as previously described. Degree of entire nucleic acid homology between both isolates was in the range of 84%. The Large Intergenic Region showed most pronounced differences while the RepA gene was most conserved. No intermediate forms were found, though both isolates co-existed in the same hosts. Sequence data lead to the suggestion that they should be referred to as different viruses rather than strains of a virus.

Keywords: Wheat dwarf virus; isolates; sequence; hosts

Published: June 30, 2002  Show citation