Plant Protect. Sci., 2002, 38(10):S49-S55 | DOI: 10.17221/10319-PPS

Spread of fire blight in Austria and Hungary - variability of Erwinia amylovora strainsOriginal Paper

M. Keck, M. Hevesi, W. Ruppitsch, A. Stöger, S. Richter
1 Austrian Agency for Health and Food Safety (AGES), A-1226 Vienna, Austria
2 Szent Istvan University, Department of Fruit Science, H-1118 Budapest, Hungary Tel.: +431 732 165 146, Fax: +431 732 165 194, E-mail: marianne.keck@lw.vie.ages.at

First outbreaks of fire blight were detected in Austria in 1993 and in Hungary in 1995. 137 Erwinia amylovora isolates were compared by PCR-RFLP, sequencing of the repeat region of the PstI fragment of plasmid pEA29, RAPD and AFLP analysis. Differences of repeat numbers in first isolates were observed. Six isolates showed different RAPD profiles. In AFLP analysis, so far, no differences in fragment pattern were determined. Pathogenicity tests with micropropagated apple cultivars revealed differences in plant susceptibility and in virulence of isolates. Responses of Malus tissue were visualized by the use of epifluorescence microscopy through the localization and the production of substances involved in cellular defence mechanisms (e.g. flavonoids, lignin) in various susceptible cultivars. The effect of bacterial strains differing in pathogenicity is shown by cellular peroxidase and flavonoid production.

Keywords: Erwinia amylovora; Austria; Hungary

Published: June 30, 2002  Show citation

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Keck M, Hevesi M, Ruppitsch W, Stöger A, Richter S. Spread of fire blight in Austria and Hungary - variability of Erwinia amylovora strains. Plant Protect. Sci. 2002;38(SI 1 - 6th Conf EFPP):S49-55. doi: 10.17221/10319-PPS.
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