Plant Protect. Sci., 2002, 38(10):S95-S98 | DOI: 10.17221/10327-PPS

The influence of jasmonic acid on the amount and the distribution of cysteine proteinase PLCP-2 in healthy and PVYNTN infected potato plants (Solanum tuberosum L.)Original Paper

M. Pompe-Novak, M. Tušek-Žnidarič, B. Štrukelj, M. Ravnikar
1 National Institute of Biology, 1000 Ljubljana, Slovenia
2 Jožef Stefan Institute, 1000 Ljubljana, Slovenia
3 Faculty of Pharmacy, University of Ljubljana, 1000 Ljubljana, Slovenia Tel.: +386 1 423 3388, Fax: +386 1 257 3847, E-mail: marusa.pompe@uni-lj.si

The localization of cysteine proteinase PLCP-2 was investigated in potato plants (Solanum tuberosum L.) cultivar Désirée by electron microscopy. Healthy and PVYNTN infected potato plants were grown in vitro on media with or without a supplement of jasmonic acid. We had already shown that PLCP-2 is present in leaves, stems, tips of shoots and tips of roots of healthy and PVYNTN infected plants. It was detected in various cell types in protein bodies in vacuoles, in cytoplasm and in cell walls. There were significantly larger amounts of PLCP-2 in plants grown on medium with a supplement of jasmonic acid in both healthy and virus infected plants. More protein bodies in vacuoles were found in plants grown on medium with addition of jasmonic acid.

Keywords: Potato virus YNTN; potato (Solanum tuberosum L.); cysteine proteinase PLCP-2; jasmonic acid; immunolocalization; electron microscopy

Published: June 30, 2002  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Pompe-Novak M, Tušek-Žnidarič M, Štrukelj B, Ravnikar M. The influence of jasmonic acid on the amount and the distribution of cysteine proteinase PLCP-2 in healthy and PVYNTN infected potato plants (Solanum tuberosum L.). Plant Protect. Sci. 2002;38(SI 1 - 6th Conf EFPP):S95-98. doi: 10.17221/10327-PPS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. AGRAWAL G.K., RAKWAL R., JWA N.S., AGRAWAL V.P. (2002): Oryza sativa proteinase inhibitor gene, OsPIN, whose mRNA specifically accumulates in a compatible host-blast pathogen interaction. Plant Physiol. Biochem., 40: 175-182. Go to original source...
    2. AVROVA A.O., STEWART H.E., DE JONG W., HEILBRONN J., LYON G.D., BIRCH P.R.J. (1999): A cysteine protease gene is expressed early in resistant potato interactions with Phytophthora infestans. Mol. Plant Microbe In., 12: 1114-1119. Go to original source... Go to PubMed...
    3. BAEBLER Š., CAMLOH M., KOVAČ M., RAVNIKAR M., ŽEL J. (2002): Jasmonic acid stimulates taxane production in cell suspension culture of yew (Taxus × media). Planta Med., 68: 475-476. Go to original source... Go to PubMed...
    4. CAMLOH M., RAVNIKAR M., ŽEL J. (1996): Jasmonic acid promotes division of fern protoplasts, elongation of rhizoids and early development of gametophytes. Physiol. Plant., 97: 659-664. Go to original source...
    5. FARMER E.E., RYAN C.A. (1992): Octadecanoid precursors of jasmonic acid activate the synthesis of wound-inducible proteinase inhibitors. Plant Cell, 4: 129-134. Go to original source...
    6. G RUDEN K., Š TRUKELJ B., R AVNIKAR M., P OLJAK PRIJATELJ M., MAVRIČ I., BRZIN J., PUNGERČAR J., KREGAR I. (1997): Potato cysteine proteinase inhibitor gene family: molecular cloning, characterisation and immunocytochemical localisation studies. Plant Mol. Biol., 34: 317-323. Go to original source... Go to PubMed...
    7. K OIWA H., B RESSAN R.A., H ASEGAWA P.M. (1997): Regulation of protease inhibitors and plant defense. Trends Plant Sci., 2: 379-384. Go to original source...
    8. KOVAČ M., RAVNIKAR M. (1998): Sucrose and jasmonic acid interact in photosynthetic pigment metabolism and development of potato (Solanum tuberosum L. cv. Sante) grown in vitro. Plant Growth Regul., 24: 101-107.
    9. K REFT S., R AVNIKAR M., M EŠKO P., P UNGEČAR J., UMEK A., KREGAR I., ŠTRUKELJ B. (1997): Jasmonic acid inducible aspartic proteinase inhibitors from potato. Phytochemistry, 44: 1001-1006. Go to original source... Go to PubMed...
    10. POMPE-NOVAK M., POLJAK-PRIJATELJ M., POPOVIČ T., ŠTRUKELJ B., RAVNIKAR M. (2002): The impact of potato cysteine proteinases in plant growth and development. Physiol. Mol. Plant Pathol., 60: 71-78. Go to original source...
    11. RAVNIKAR M., VILHAR B., GOGALA N. (1992): Stimulatory effects of jasmonic acid on potato stem node and protoplast culture. J. Plant Growth Regul., 11: 29-33. Go to original source...
    12. SHIN R., LEE G.J., PARK C.J., KIM T.Y., YOU J.S., NAM Y.W., PEAK K.H. (2001): Isolation of peper mRNAs differentially expressed during the hypersensitive response to tobacco mosaic virus and characterization of a proteinase inhibitor gene. Plant Sci., 161: 727-737. Go to original source...
    13. VIERSTRA R.D. (1993): Protein degradation in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol., 44: 385-410. Go to original source...
    14. VIERSTRA R.D. (1996): Proteolysis in plants: mechanisms and function. Plant Mol. Biol., 32: 275-302. Go to original source...
    15. V ILHAR B., R AVNIKAR M., S CHARA M., N EMEC M., G OGALA N. (1991): The influence of jasmonic acid on biophysical properties of potato leaf protoplasts and roots. Plant Cell Rep., 10: 541-544. Go to original source... Go to PubMed...
    16. VILHAR B., DERMASTIA M., RAVNIKAR M. (1995): Jasmonic acid and sucrose affect patterns of soluble leaf proteins in potato. Acta Chim. Slov., 42: 479-485.

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content