Plant Protect. Sci., 2002, 38(10):161-164 | DOI: 10.17221/10345-PPS

Selection of AFLP markers linked with crown rust resistance in Lolium: efficiency of bulk segregant analysis in an Allogamous speciesOriginal Paper

H. Muylle, E. Van Bockstaele, I. Roldan-Ruiz
1 Department of Plant Genetics and Breeding, CLO-Gent, 9090 Melle, Belgium
2 Department of Plant Production, University Gent, 9000 Gent, Belgium Tel.: +32 9 272 2839, Fax: +32 9 272 2901, E-mail: h.muylle@clo.fgov.be

Ryegrasses are important forage species. An important foliar disease in Lolium is crown rust, caused by the fungus Pucciniacoronata. This cosmopolitan biotrophic fungus causes forage and seed losses, and decoloration of amenity grasslands.In breeding towards resistant cultivars, it is important to know the mode of inheritance of crown rust resistance. A paircross between a susceptible and a resistant plant resulted in a F1 population segregating for rust resistance. According tothe segregation data, major genes were assumed to encode for crown rust resistance. In a bulk segregant analysis (BSA)187 primer combinations revealed AFLP markers linked with resistance. Two groups of genetically unlinked markerswere identified, explaining together 35% of the phenotypic variation.

Keywords: L. perenne; Puccinia coronata; BSA; molecular markers

Published: June 30, 2002  Show citation

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Muylle H, Van Bockstaele E, Roldan-Ruiz I. Selection of AFLP markers linked with crown rust resistance in Lolium: efficiency of bulk segregant analysis in an Allogamous species. Plant Protect. Sci. 2002;38(SI 1 - 6th Conf EFPP):161-164. doi: 10.17221/10345-PPS.
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