Plant Protect. Sci., 2002, 38(10):173-176 | DOI: 10.17221/10348-PPS

Viroids: sequence variability and evolution of pathogenic RNAOriginal Paper

J. Matoušek
Institute of Plant Molecular Biology Academy of Sciences of the Czech Republic, 370 05 České Budějovice, Czech Republic

Viroids as the smallest pathogenic circular single-stranded pathogenic RNAs form populations of quasi-species, whichhas been recently identified by thermodynamic methods like TGGE pre-selection and heteroduplex analysis. It was foundthat replication under thermal stress led to enormously high level of viroid mutagenesis. Mostly multiple mutants havingnon-random distribution of base changes were found. A specific "hot spots" were identified in the regions, wherea characteristic "pathogenicity domains" are localised in different viroids of the pospiviroidae family. Specific viroidmicroevolution was observed upon artificial inoculation of non-host plant species. Our results suggest that viroid propagationunder physiological stress can be assumed as important factor, which is among others, responsible for an appearanceof viroid quasi-species in the nature. Evolution and new viroid patotypes could accumulate due to environmental stressincluding various pollutants may be a potential danger for cultured plants.

Keywords: viroid mutagenesis; quasi-species; DNA heteroduplexes; stress factors; viroid pathotypes

Published: June 30, 2002  Show citation

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Matoušek J. Viroids: sequence variability and evolution of pathogenic RNA. Plant Protect. Sci. 2002;38(SI 1 - 6th Conf EFPP):173-176. doi: 10.17221/10348-PPS.
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