Plant Protect. Sci., 2002, 38(10):183-185 | DOI: 10.17221/10351-PPS

Molecular studies of Potato mop-top virus (PMTV) in transgenic N. benthamiana and S. tuberosumOriginal Paper

A. Germundsson, M. Sandgren, H. Barker, E.I. Savenkov, J.P.T. Valkonen
1 Department of Plant Biology, Genetics Centre, SLU, S-750 07 Uppsala, Sweden
2 Department of Applied Biology, University of Helsinki, FIN-00014 Helsinki, Finland
3 Scottish Crop Research Institute, Invergowrie, DD2 5DA Dundee, United Kingdom Tel.: +46 18 673 372, Fax: +46 18 673 392, E-mail: jari.valkonen@vbiol.slu.se

Resistance to Potato mop-top virus (PMTV) was studied in Nicotiana benthamiana and potato (Solanum tuberosum cv.Saturna) transformed with the coat protein (CP) gene of PMTV. In N. benthamiana plants mechanically inoculated withPMTV, RNA2 was detected in leaves and roots in several plants in the absence of RNA3. When N. benthamiana wasgrown in infested soil, viral RNA was detected in roots, but no systemic movement of PMTV to above-ground parts wasobserved. The incidence of PMTV infections was reduced in the CP-transgenic potato plants grown in an infested fieldin Sweden. However, in infected tubers, all three virus RNAs were detected.

Keywords: Potato mop-top virus; Spongospora subterranea; Solanum tuberosum; Nicotiana benthamiana; transgenic resistance

Published: June 30, 2002  Show citation

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Germundsson A, Sandgren M, Barker H, Savenkov EI, Valkonen JPT. Molecular studies of Potato mop-top virus (PMTV) in transgenic N. benthamiana and S. tuberosum. Plant Protect. Sci. 2002;38(SI 1 - 6th Conf EFPP):183-185. doi: 10.17221/10351-PPS.
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    References

    1. BARKER H., REAVY B., MCGEACHY K.D. (1998): High level of resistance in potato to potato mop-top virus induced by transformation with the coat protein gene. Eur. J. Plant Pathol., 104: 737-740. Go to original source...
    2. GERMUNDSSON A., SANDGREN M., BARKER H., SAVENKOV E.I., VALKONEN J.P.T. (2002): Initial infection of roots and leaves reveals different resistance phenotypes associated with the coat protein gene-mediated resistance to Potato mop-top virus. J. Gen. Virol., 83: 1201-1209. Go to original source... Go to PubMed...
    3. LAUBER E., BLEYKASTEN-GROSSHANS C., ERHARDT M., BOUZOUBAA S., JONARD G., RICHARDS K.E., GUILLEY H. (1998): Cell-to-cell movement of beet necrotic yellow vein virus: I. Heterologous Complementation Experiments provide evidence for specific interactions among the triple gene block proteins. MPMI, 11: 618-625. Go to original source... Go to PubMed...
    4. REAVY B., ARIF M., KASHIWAZAKI S., WEBSTER K.D., BARKER H. (1995): Immunity to potato mop-top virus in Nicotiana benthamiana plants expressing the coat protein gene is effective against fungal inoculation of the virus. MPMI, 8: 286-291. Go to original source... Go to PubMed...
    5. REAVY B., ARIF M., COWAN G.H., TORRANCE L. (1998): Association of sequences in the coat protein/readthrough domain of potato mop-top virus with transmission by Spongospora subterranea. J. Gen. Virol., 79: 2343-2347. Go to original source... Go to PubMed...
    6. S COTT K.P., K ASHIWAZAKI S., R EAVY B., H ARRISON B.D. (1994): The nucleotide sequence of potato mop-top virus RNA2: a novel type of genome organization for a furovirus. J. Gen. Virol., 75: 3561-3568. Go to original source... Go to PubMed...
    7. SAVENKOV E.I., SANDGREN M., VALKONEN J.P.T. (1999): Complete sequence of RNA1 and presence of tRNA-like structures in all RNAs of potato mop-top virus. J. Gen. Virol., 80: 2779-2784. Go to original source... Go to PubMed...
    8. VERWOERD T.C., DEKKER B.M.M., HOEKEMA A. (1989): A small scale procedure for rapid isolation of plants RNAs. Nuc. Acid. Res., 17: 2362. Go to original source... Go to PubMed...

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