Plant Protect. Sci., 2002, 38(10):205-208 | DOI: 10.17221/10356-PPS
Antibiotic production of the biocontrol agents Epicoccum nigrum and Candida sakeOriginal Paper
- Department of Plant Protection, INIA, Carretera, 28040 Madrid, Spain * Tel.: +34 91 3 476 846, Fax: +34 91 3 572 293, E-mail: melgar@inia.es
In the framework of the study of the mode of action of biocontrol agents (BCAs) it is important to know if BCAs areantibiotic-producers. Epicoccum nigrum 282 and Candida sake CPA-1 are BCAs effective against post-harvest pathogensof stone and pome fruits. The antibiotics produced by these BCAs and the relationship to biocontrol were studied.Production of antibiotics by E. nigrum in in vitro cultures began at 5 days of incubation being maximal at different timesdepending on media used. However, no antibiotic was detected when E. nigrum was grown in a solid state-fermentationsystem or in peaches. In the case of C. sake, no antibiotic was detected either in vitro, in liquid fermentation culturesof the yeast, or in apples.
Keywords: apples; antibiotic; biocontrol; Monilinia spp.; Penicillium expansum; peaches; post-harvest
Published: June 30, 2002 Show citation
References
- BROWN A.E., FINLAY R., WARD J. (1987): Antifungal compounds produced by Epicoccum purpurascens against soil-borne plant pathogenic fungi. Soil Biol. Biochem., 19: 657-664.
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- DE CAL A., M.-SAGASTA E., MELGAREJO P. (1988): Antifungal substances produced by Penicillium frequentans and their relationship to the biocontrol of Monilinia laxa. Phytopathology, 78: 888-893.
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- DE CAL A., LARENA I., MELGAREJO P. (2001): (Inventors), Spain. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (Assignee). Procedimiento para la producción de conidias de un hongo filamentoso en fermentación sólida. Patent number 2001100758.
- MADRIGAL C., TADEO J.L., MELGAREJO P. (1991): Relationship between flavipin production by Epicoccum nigrum and antagonism against Monilinia laxa. Mycol. Res., 95: 1375-1381.
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