Plant Protect. Sci., 2002, 38(11):255-257 | DOI: 10.17221/10459-PPS

Electronmicroscopic study of Beet soilborne pomovirus

P. Kudláčková, M. Zouhar, P. Ryšánek
Department of Plant Protection, Czech University of Agriculture Prague,
1 65 21 Prague-Suchdol, Czech Republic

Beet soilborne pomovirus (BSBV) was observed both in the sap and in tissues from local lesions on Chenopodium quinoa leaves after their embedding into acrylic resin LR White. Immunocapturing with polyclonal antibodies was used to enhance number of particles on grids and immunolabelling by colloidal gold was used for better visibility of virus particles in tissues. BSBV has rod-like particles of various length and it forms inclusions of several particles adhering side to side each to another.

Keywords: Beet soilborne virus; Chenopodium quinoa; polyclonal antibodies; immunolabelling; electron microscopy

Published: December 31, 2002  Show citation

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Kudláčková P, Zouhar M, Ryšánek P. Electronmicroscopic study of Beet soilborne pomovirus. Plant Protect. Sci. 2002;38(SI 2 - 6th Conf EFPP):255-257. doi: 10.17221/10459-PPS.
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