Plant Protect. Sci., 2002, 38(11):329-331 | DOI: 10.17221/10482-PPS

Effect of fungicides on the growth of fungi isolated from in vitro propagated fruit-bearing plants

M. Kowalik, M. Gródek
Department of Plant Protection, Agricultural University of Cracow, 31-425 Cracow, Poland

The aim of the work was to determine the effect of fungicides: Topsin M 70 WG, Bravo 500 SC, and Sportak 45 EC in two concentrations on the growth of fungi: Aspergillus niger, Epicoccum purpurascens, Cladosporium sphaerospermum, Mortierella isabellina and Penicillium verrucosum var. verrucosum on PDA and Murashige-Skoog medium (MS). The smallest index of linear growth rate of the fungi had Topsin M 70 WG for fungi: E. purpurascens and P. verrucosum var. verrucosum on PDA and MS medium and for M. isabellina and C. sphaerospermum on MS in concentrations of 71.42 mg/l and 107.13 mg/l. The highest index of linear growth had Bravo 500 SC in concentrations of 0.5 ml/l and 0.75 ml/l on PDA for E. purpurascens and A. niger. The lowest index of linear growth on MS for E. purpurascens, C. sphaerospermum and P. verrucosum var. verrucosum was noted down. The highest effectiveness in control of linear growth all tested species of fungi was found for fungicide Sportak 45 EC in concentration of 0.55 ml/l and 0.825 ml/l on MS and PDA. Among all tested fungicides used in vitro propagation fungicides from imidazoles group are recommended.

Keywords: fungi; fungicides; in vitro; micopropagation

Published: December 31, 2002  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Kowalik M, Gródek M. Effect of fungicides on the growth of fungi isolated from in vitro propagated fruit-bearing plants. Plant Protect. Sci. 2002;38(SI 2 - 6th Conf EFPP):329-331. doi: 10.17221/10482-PPS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. HERMAN E.B. (1990): Non-axente plant tissue culturae possibilites and opportunities. Acta Hortic., 280: 112-117. Go to original source...
    2. KOWALIK M., GRÓDEK M. (2000): Phytotoxicity of fungicides in relation to strawberry explants in in vitro cultures. In: Proc. XVth Czech and Slovak Plant Protection Conf., Brno: 369-370.
    3. LEIFERT C., MORRIS C.E., WAITES W.M. (1994): Ecology of microbiological saprophytes and pathogens in tissue culture and fieldgrown plants: reasons for contamination problems in vitro. Crit. Rev. Plants Sci., 13: 139-189. Go to original source...
    4. LILLY V.G., BARNETT H.L. (1959): Fizjologia grzybów. PWRiL, Warszawa.
    5. REBY E. (2001): Zakażenia powodowane przez bakterie i grzyby w kulturach in vitro i możliwości ich ograniczania. [Praca doktorska.] Akademia Rolnicza w Krakowie.
    6. REBY E., KOWALIK M. (2000): Effect of culture media on growth of fungi isolated from in vitro propagated plants. Rocz. AR Poznań, CCCXXI, Ogrodn., 30: 133-138.
    7. SHIELDS R., ROBINSON S.J., ANSLOW P.A. (1984): Use of fungicides in plant tissue culture. Plant Cell Rep., 3: 33-36. Go to original source... Go to PubMed...
    8. ZENKTELER E. (1998): Współczesne metody wykrywania i eliminowania drobnoustrojów podczas mikrorozmnażania. Biotechnologia, 40: 149-166.

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content