Plant Protect. Sci., 2002, 38(11):406-407 | DOI: 10.17221/10507-PPS

Comparison of multiplex real-time PCR and ergosterol assays in quantifying Heterobasidion annosum in planta

A.M. Hietala, M. Eikenes, M. Kvaalen, H. Solheim, C.G. Fossdal
Norwegian Forest Research Institute, 1432 Ås, Norway

A quantitative multiplex real-time PCR procedure was developed to monitor the dynamics in Norway spruce-Heterobasidion annosum pathosystem. The assay reliably detected down to 1 pg of H. annosum DNA and 1 ng of host DNA in multiplex conditions. As a comparative method for quantifying fungal colonization, we applied the ergosterol assay. There was a very high correlation between the results obtained with the two methods, this strengthening the credibility of both assays. The advantages and disadvantages of these assays are discussed.

Keywords: Heterobasidion; Norway spruce; infection; quantification

Published: December 31, 2002  Show citation

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Hietala AM, Eikenes M, Kvaalen M, Solheim H, Fossdal CG. Comparison of multiplex real-time PCR and ergosterol assays in quantifying Heterobasidion annosum in planta. Plant Protect. Sci. 2002;38(SI 2 - 6th Conf EFPP):406-407. doi: 10.17221/10507-PPS.
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    References

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