Plant Protect. Sci., 2002, 38(11):480-482 | DOI: 10.17221/10529-PPS

Characterization of two closely linked soybean pgip genes and transcript regulation following pathogen infection and wounding

D. R Ovidio, S. Roberti, M. Melaragni, C. Capodicasa, L.S. Sella, F. Favaron
1 Dipartimento di Agrobiologia e Agrochimica, Università della Tuscia, 01100 Viterbo, Italy
2 Dipartimento del Territorio e Sistemi Agro-Forestali, Università di Padova,
3 5020 Legnaro (PD), Italy E-mail: dovidio@unitus.it

Polygalacturonase-inhibiting protein (PGIP) is a plant cell wall protein that regulates the action of fungal endopolygalacturonases (PG) favouring the formation of oligogalacturonides active as elicitors of plant defense responses. We have isolated two novel soybean PGIP genes (Gmpgip1 and Gmpgip2) and their recognition specificities against fungal PGs have been tested by expressing them in Nicotiana benthamiana using the Potato virus X (PVX) as vector and in Escherichia coli. In both systems GmPGIP1 and GmPGIP2 accumulate but showed no activity against PGs. Transcript regulation of GmPGIP1 and GmPGIP2 showed that these genes undergo a differential regulation following Sclerotinia sclerotiorum infection and wounding.

Keywords: Sclerotinia sclerotiorum; Glycine max; PGIP; polygalacturonase; gene family; transcript regulation

Published: December 31, 2002  Show citation

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Ovidio DR, Roberti S, Melaragni M, Capodicasa C, Sella LS, Favaron F. Characterization of two closely linked soybean pgip genes and transcript regulation following pathogen infection and wounding. Plant Protect. Sci. 2002;38(SI 2 - 6th Conf EFPP):480-482. doi: 10.17221/10529-PPS.
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    References

    1. BAULCOMBE D.C., CHAPMAN S., CRUZ S.S. (1995): Jellyfish green fluorescent protein as a reporter for virus infections. Plant J., 7: 1045-1053. Go to original source... Go to PubMed...
    2. DE LORENZO G., D'OVIDIO R., CERVONE F. (2001). The role of Polygalacturonase-Inhibiting Proteins (PGIPs) in defence against pathogenic fungi. Annu. Rev. Phytopathol., 39: 313-335. Go to original source... Go to PubMed...
    3. FAVARON F., D'OVIDIO R., PORCEDDU E., ALGHISI P. (1994): Purification and molecular characterization of a soybean polygalacturonase-inhibiting protein (PGIP). Planta, 195: 80-87. Go to original source... Go to PubMed...
    4. LECKIE F., MATTEI B., CAPODICASA C., HEMMINGS A., NUSS L., ARACRI B., DE LORENZO G., CERVONE F. (1999): The specificity of polygalacturonase-inhibiting protein (PGIP): a single amino acid substitution in the solvent-exposed β-strand/β-turn region of the leucin-rich repeats (LRRs) confers a new recognition capability. EMBO J., 18: 2352-2363. Go to original source... Go to PubMed...
    5. MAHALINGAM R., WANG G., KNAP H.T. (1999): Polygalacturonase and polygalacturonase inhibitor protein: gene isolation and transcription in Glycine max-Heterodera glycines interactions. Mol. Plant Microbe In., 12: 490-498. Go to original source... Go to PubMed...
    6. POWELL A.L.T., VAN KAN J., TEN HAVE A., VISSER J., G REVE L.C., B ENNETT A.B., L ABAVITCH J.M. (2000): Transgenic expression of pear PGIP in tomato limits fungal colonization. Mol. Plant Microbe In., 13: 942-950. Go to original source... Go to PubMed...

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