Plant Protect. Sci., 2002, 38(11):483-486 | DOI: 10.17221/10530-PPS

Isolation of differentially expressed genes involved in clubroot disease

A. Schuller, J. Ludwig-Müller
Institut für Botanik, Technic University Dresden, 01062 Dresden, Germany

The interaction between Plasmodiophora brassicae and its host Brassica rapa is investigated by two strategies. (1) IAA-conjugate hydrolases: Root hypertrophy in club root disease is dependent on increased auxin levels and these could result from auxin-conjugate hydrolysis. So far we isolated 5 different cDNA fragments out of various tissues which revealed high identity to IAR3/ILL5, ILL2, ILL3, ILL6 and ILR1 genes from Arabidopsis by comparison with database entries. (2) Random priming: Using this method, we have so far obtained 26 clones from clubroot tissue, from which several sequences may be components of plant signal transduction chains, metabolic pathways and transcriptional regulation.

Keywords: Plasmodiophora brassicae; Brassica rapa; IAA-amidohydrolases; random priming

Published: December 31, 2002  Show citation

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Schuller A, Ludwig-Müller J. Isolation of differentially expressed genes involved in clubroot disease. Plant Protect. Sci. 2002;38(SI 2 - 6th Conf EFPP):483-486. doi: 10.17221/10530-PPS.
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