Plant Protect. Sci., 2002, 38(11):502-503 | DOI: 10.17221/10535-PPS

Host gene expression at an early stage of virus resistance induction

E. GAMMELGÅRD, M.L. Mohan, R.A. Andersson, J.P.T. Valkonen
1 Department of Plant Biology, Swedish University of Agricultural Sciences, SE-750 07 Uppsala, Sweden
2 Department of Applied Biology, University of Helsinki, FIN-00014 Helsinki, Finland E-mail: Jari.Valkonen@vbiol.slu.se

Suppression subtractive hybridization (SSH) was carried out to detect genes differentially expressed in plants expressing resistance to systemic infection with Potato virus A (PVA), genus Potyvirus. Differential screening has up to now revealed 19 putative differentially expressed genes. Nothern blot hybridization has confirmed the differential expression of seven genes. Three of them were only induced by the virus, but four genes were also wound-induced.

Keywords: PVA; Solanum tuberosum; suppression subtractive hybridization

Published: December 31, 2002  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
GAMMELGÅRD E, Mohan ML, Andersson RA, Valkonen JPT. Host gene expression at an early stage of virus resistance induction. Plant Protect. Sci. 2002;38(SI 2 - 6th Conf EFPP):502-503. doi: 10.17221/10535-PPS.
Download citation

References

  1. DIATCHENKO L., YUN-FAI C.L., CAMPBELL A.P., CHENCHIK A., M OQADAM F., H UANG B., L UKYANOV K., GURSKAYA N., SVERDLOV E.D., SIEBERT P.D. (1996): Suppression subtractive hybridisation: a method for generating differentially regulated or tissue-specific cDNA probes and libraries. P. Natl. Acad. Sci. USA, 93: 6025-6030. Go to original source... Go to PubMed...
  2. H ÄMÄLÄINEN J.H., K EKARAINEN T., G EBHARDT C., WATANABE K.N., VALKONEN J.P.T. (2000): Recessive and dominant resistance interfere with the vascular transport of Potato virus A in diploid potatoes. Mol. Plant Microbe In., 13: 402-412. Go to original source... Go to PubMed...
  3. RÄJAMÄKI M.-L., VALKONEN J.P.T. (1999): The 6K2 protein and the VPg of Potato virus A are determinants of systemic infection in Nicandra physaloides. Mol. Plant Microbe In., 12: 1074-1081. Go to original source... Go to PubMed...

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.