Plant Protect. Sci., 2002, 38(11):504-506 | DOI: 10.17221/10536-PPS

Temporal expression of a PGIP-gene in strawberry cultivars induced by wounding or by Botrytis cinerea infection

L. Mehli
Plantebiosenteret, Department of Botany, Norwegian University of Technology and Science (NTNU), 7491 Trondheim, Norway

The expression of a PGIP gene (polygalacturonase inhibitor protein) was monitored with semi-quantitative (SQ)-RT-PCR in green, white and red berries of the strawberry cultivar Korona upon infection with Botrytis cinerea and wounding. In addition, the PGIP expression in infected white berries was quantified in four additional cultivars. The constitutive expression of PGIP increased from green to red berries in Korona suggesting developmental regulation of the gene. Wounding and fungal infection caused a moderate or a high induction in the PGIP level, respectively. The maximum peak was observed 24 h after the treatments. In the comparative experiment with five cultivars, infection of white berries caused an induction in the PGIP level 24 h after inoculation in four out of five cultivars.

Keywords: strawberry; Botrytis cinerea; polygalacturonase inhibitor protein

Published: December 31, 2002  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Mehli L. Temporal expression of a PGIP-gene in strawberry cultivars induced by wounding or by Botrytis cinerea infection. Plant Protect. Sci. 2002;38(SI 2 - 6th Conf EFPP):504-506. doi: 10.17221/10536-PPS.
Download citation

References

  1. BÉZIER A., LAMBERT B., BAILLIEUL F. (2002): Study of defense related gene expression in grapevine leaves and berries infected with Botrytis cinerea. Eur. J. Plant Pathol., 108: 111-120. Go to original source...
  2. DAUGAARD H. (1999): Cultural methods for controlling Botrytis cinerea Pers. in strawberry. Biol. Agric. Hortic., 16: 351-361. Go to original source...
  3. MACHINANDIARENA M.F., OLVIERI F.P., DALEO G.R., OLIVA C.R. (2001): Isolation and characterization of a polygalacturonase-inhibiting protein from potato leaves. Accumulation in response to salicylic acid, wounding and infection. Plant Physiol. Biochem., 39: 129-136. Go to original source...
  4. POWELL A.L.T., VAN KAN J., TEN HAVE A., VISSER J., CARL GREVE L., BENNETT A., LABAVITCH J.M. (2000): Transgenic expression of pear PGIP in tomato limits fungal colonization. Mol. Plant Microbe In., 13: 942-950. Go to original source... Go to PubMed...
  5. SCHAART J.G., SALENTIJN E.M.J., KRENS F.A. (2002): Tissue-specific expression of the β-glucuronidase reporter gene in transgenic strawberry (Fragaria × ananassa) plants. Plant Cell Rep. (Forthcoming). Go to original source...
  6. SCHULTZ D.J., CRAIG R., COX-FOSTER D.L., MUMMA R.O., MEDFORD J.J. (1994): RNA isolation from recalcitrant plant tissue. Plant Mol. Biol. Rep., 12: 310-316. Go to original source...

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.