Plant Protect. Sci., 2002, 38(11):523-527 | DOI: 10.17221/10544-PPS

The role of type III secretion system in Erwinia carotovora subsp. atroseptica virulence

A.V. Ageichik, A.N. Evtushenkov, Y.A. Nikolaichik
Department of Microbiology, Faculty of Biology, Belarus State University, 220080 Minsk, Belarus

A hrpJ mutant of Erwinia carotovora subsp. atroseptica (Eca) was constructed and the effects of this mutation on induction of the hypersensitivity response and virulence of Eca were studied. The hrpJ mutant does not induce the HR in non-host plant Vicia faba (V. faba) and demonstrates cultivar-dependent increase or decrease of virulence on potato plants.

Keywords: Erwinia carotovora subsp. atroseptica; potato; type III secretion system; virulence; hypersensitive response

Published: December 31, 2002  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Ageichik AV, Evtushenkov AN, Nikolaichik YA. The role of type III secretion system in Erwinia carotovora subsp. atroseptica virulence. Plant Protect. Sci. 2002;38(SI 2 - 6th Conf EFPP):523-527. doi: 10.17221/10544-PPS.
Download citation

References

  1. AUSUBEL F.A., BRENT R., KINGSTON R.E., MOORE D.D., SEIDMAN J.G., SMITH J.A., STRUHL K. (1992): Current protocols in molecular biology. Greene Publishing Associates and Wiley-Interscience Press, New York.
  2. BAUER D.W., BOGDANOVE A.J., BEER S.V., COLLMER A. (1994): Erwinia chrysanthemi hrp genes and their involvement in soft rot pathogenesis and elicitation of the hypersensitive response. Mol. Plant Microbe In., 7: 573-581. Go to original source... Go to PubMed...
  3. BAIN R.A., PEROMBELON M.C.M. (1988): Methods of testing potato cultivars for resistance to soft rot of tubers caused by Erwinia carotovora subsp. atroseptica. Plant Pathol., 37: 431-437. Go to original source...
  4. BELL K.S., AVROVA A.O., HOLEVA M.C., CARDLE L., M ORRIS W., D E J ONG W., T OTH I.K., W AUGH R., BRYAN G.J., BIRCH P.R. (2002): Sample sequencing of a selected region of the genome of Erwinia carotovora subsp. atroseptica reveals candidate phytopathogenicity genes and allows comparison with Escherichia coli. Microbiology, 148: 1367-1378. Go to original source... Go to PubMed...
  5. BULLOCK W.O., FERNANDEZ J.M., STUART J.M. (1987): XL1-Blue: a high efficiency plasmid transforming recA Escherichia coli strain with beta-galactosidase selection. Biotechnology, 5: 376-379.
  6. FLOR H.H. (1956): The complementary genic systems in flax and flax rust. Adv. Genet., 8: 29-54. Go to original source...
  7. HUANG H.C., XIAO Y., LIN R.H., LU Y., HUTCHESON S.W., C OLLMER A. (1993): Characterization of the Pseudomonas syringae pv. syringae 61 hrpJ and hrpI genes: homology of HrpI to a superfamily of proteins associated with protein translocation. Mol. Plant Microbe In., 6: 515-520. Go to original source... Go to PubMed...
  8. L INDGREN P.B. (1997): The role of hrp genes during plant-bacterial interactions. Annu. Rev. Phytopathol., 35: 129-152. Go to original source... Go to PubMed...
  9. LINDGREN P.B., PEET R.C., PANOPOULOS N.J. (1986): Gene cluster of Pseudomonas syringae pv. "phaseolicola" controls pathogenicity of bean plants and hypersensitivity of nonhost plants. J. Bacteriol., 168: 512-522. Go to original source... Go to PubMed...
  10. METCALF W.W., GIANGJ W., WANNER B.L. (1994): Use of the rep technique for allele replacement to constrict new Escherichia coli hosts for maintenance of R6K gamma origin plasmids at different copy numbers. Gene, 138: 1-7. Go to original source... Go to PubMed...
  11. NORRANDER I., KEMPE T., MESSING I. (1983): Construction of improved M13 vectors using oligodeoxynucleotide directed mutagenesis. Gene, 26: 101-106. Go to original source... Go to PubMed...
  12. PRENTKI P., KRISCH H.M. (1984): In vitro insertional mutagenesis with a selectable DNA fragment. Gene, 29: 303-313. Go to original source... Go to PubMed...
  13. QUANDT J., HYNES M.F. (1993): Versatile suicide vectors which allow direct selection for gene replacement in Gram-negative bacteria. Gene, 127: 15-21. Go to original source... Go to PubMed...
  14. RANTAKARI A.O., VIRTAHARJU O., VAHAMIKO S., TAIRA S., PALVA E.T., SAARILAHT H.T., ROMANTSHUK M. (2001): Type III secretion contributes to the pathogenesis of the soft-rot pathogen Erwinia carotovora: partial characterization of the hrp gene cluster. Mol. Plant Microbe In., 14: 962-968. Go to original source... Go to PubMed...
  15. SHEVCHIK V.E., BOCCARA M., VEDEL R., HUGOUVIEUXCOTTE-PATTAT N. (1998): Processing of the pectate lyase PelI by extracellular proteases of Erwinia chrysanthemi 3937. Mol. Microbiol., 29: 1459-1469. Go to original source... Go to PubMed...
  16. WEI Z.M., BEER S.V. (1993): HrpI of Erwinia amylovora functions in secretion of harpin and is a member of a new protein family. J. Bacteriol., 175: 7958-7967. Go to original source... Go to PubMed...

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.