Plant Protect. Sci., 2002, 38(11):721-722 | DOI: 10.17221/10599-PPS

Comparison of gene expression in Solanum bulbocastanum infected with virulent and avirulent isolates of Meloidogyne chitwoodi

J. Wishart, M.S. Phillips, A. Paterson, V.C. Blok
Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, Scotland

Resistance to root knot nematode M. chitwoodi has been identified in the wild tuber-bearing Solanum species, S. bulbocastanum. Three pathotypes were identified suggesting at least two different genetic factors for virulence and resistance in the pathogen and the host species, respectively. Roots of S. bulbocastanum were infested with two isolates of M. chitwoodi differing in virulence. The infection process was monitored by histological examination of roots allowing time points to be identified. cDNA libraries were constructed from infected root tissue using Suppression Subtractive Hybridisation (SSH) to enrich for transcripts from either compatible or incompatible interactions, at three days and seven days post infection. Both plant and nematode genes, which may be important during the host/parasite interaction, were identified.

Keywords: Meloidogyne chitwoodi; Solanum bulbocastanum; suppression subtractive hybridisation; virulence genes

Published: December 31, 2002  Show citation

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Wishart J, Phillips MS, Paterson A, Blok VC. Comparison of gene expression in Solanum bulbocastanum infected with virulent and avirulent isolates of Meloidogyne chitwoodi. Plant Protect. Sci. 2002;38(SI 2 - 6th Conf EFPP):721-722. doi: 10.17221/10599-PPS.
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