Plant Protect. Sci., 2003, 39(1):7-12 | DOI: 10.17221/3820-PPS

Phytoplasma occurrence in apple trees in the Czech Republic

Renata Fialová, Milan Navrátil, Pavla Válová
Department of Cell Biology and Genetics, Faculty of Science, Palacký University, Olomouc-Holice, Czech Republic

The presence of phytoplasmas in apple trees with proliferation symptoms, rubbery wood symptoms and no symp­toms was determined by using polymerase chain reaction assays with primers amplifying phytoplasma 16S rRNA gene. Phytoplasmas were detected in all trees with proliferation symptoms. Positive tests for phytoplasma in the group of trees with rubbery wood symptoms and of those without symptoms revealed a relatively high incidence of latent phytoplasma infection. Using restriction fragment length polymorphism analysis, phytoplasma of the same identity - apple proliferation phytoplasma (subgroup 16SrX-A) - was recorded in all positively tested trees.

Keywords: proliferation symptoms; rubbery wood symptoms; non-symptomatic infection; PCR; RFLP

Published: March 31, 2003  Show citation

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Fialová R, Navrátil M, Válová P. Phytoplasma occurrence in apple trees in the Czech Republic. Plant Protect. Sci. 2003;39(1):7-12. doi: 10.17221/3820-PPS.
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    References

    1. A U., S E. (1992): Detection of DNA of plant pathogenic mycoplasmalike organisms by a polymerase chain reaction that amplifies a sequence of the 16S rRNA gene. Phytopathology, 82: 828-832. Go to original source...
    2. A A., R E., O R., P S. (1972): Bodies related to mycoplasmas in apple plants infected with witches' broom disease. Rivista Patol. Veget., 8: 3-19.
    3. B A.B., M M.D., P A.F., S G.C. (1971): Mycoplasma-like organisms associated with chat fruit and rubbery wood diseases of apple, Malus domestica Borkh., compared with those in strawberry with green petal disease. J. Gen. Microbiol., 66: 55-62. Go to original source...
    4. B A., V M., J M., F -H J. (1998): Molecular detection of phytoplasmas in apple with rubbery wood symptoms. Acta Hortic., 472: 693-700. Go to original source...
    5. B A., D D., F R., F J., K R., M M., N M., P S., B M. (2001): A molecular survey to identify phytoplasmas associated with apple trees showing different diseases symptoms. Acta Hortic., 550: 371-376. Go to original source...
    6. B C., S V., E M. (1963): The apple proliferation of various sorts and possible strain differentiation of the virus. Phytopath. Medit., 2: 116-123.
    7. B K., K G. (1995): Epidemiological studies on apple proliferation disease in southern Germany. Acta Hortic., 386: 444-447. Go to original source...
    8. B J., K O., S V. (1972): Mycoplasmas in apple trees infected with the proliferation disease. Biol. Plantarum, 14: 427-429. Go to original source...
    9. C O. (1973): Confirmation of the occurrence of mycoplasma-like micro-organisms in the phloem of apple trees with witches' broom disease. Phytopath. Z., 76: 30-32.
    10. EPPO/CABI (1997): Apple proliferation Phytoplasma. In: S I.M., M N D.G., S P.R., H M. (eds): Quarantine Pests for Europe. 2nd ed. CAB Int., Wallingford, UK.
    11. F H., B G. (1982): Investigation on the spread of witches' broom disease of apples in west and south German orchards. Erwerbsobstbau, 11: 261-265.
    12. G J., M G., V C. (1968): Microorganismes de type mycoplasme dans les cellules libérienne de Malus sylvestris L. a eint de la maladie des proliferations. C. R. Acad. Sc. Paris, 267: 76-77.
    13. L I.M., B A., V M., G D.E. (1995): Detection of multiple phytoplasmas in perennial fruit trees with decline symptoms in Italy. Phytopathology, 85: 728-735. Go to original source...
    14. L, I.M., G -R D.E., D R.E., B I.M. (1998): Revised classification scheme of phytoplasmas based on RFLP analyses of 16S rRNA and ribosomal protein gene sequences. Int. J. Syst. Bacter., 48: 1153-1169. Go to original source...
    15. L, K.H., S B., A U., S E. (1995): Detection of the apple proliferation and pear decline phytoplasmas by PCR amplification of ribosomal and nonribosomal DNA. Phytopathology, 85: 771-776. Go to original source...
    16. P H., M R. (1976): Versuche zur Infection von Apfelbäumen mit dem möglichen Erreger der Triebsucht des Apfels. Phytopath. Z., 86: 365-369. Go to original source...
    17. R E., O R., L N., R P. (1986): Research on transmission of apple proliferation. Acta Hortic., 193: 345-355. Go to original source...
    18. S E. (1990): Apple Proliferation. In: Compendium of Apple and Pear Diseases. Am. Phytopathol. Soc., St. Paul, Minnesota, USA: 67-68.
    19. S E., K H., L K.H. (1998): On the geographic distribution and prevalence of apple proliferation phytoplasma in low-intensity orchards in Germany. Z. Pflkrankh. Pflschutz., 105: 404-410.
    20. S E., K L., S U. (1984): Colonization behaviour of MLO, and symptom expression of proliferation-diseased apple trees and declinediseased pear trees over a period of several years. Z. Pflkrankh. Pflschutz., 91: 525-532.
    21. S V. (1971): Contribution to the problems of apple proliferation disease. Ann. Phytopath., No. Hors. Ser.: 391-403.
    22. S V., K V. (1977): Citlivost odrůd jabloně k mykoplazmózní proliferaci. Ochr. Rostl., 13: 231-235.

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