Plant Protect. Sci., 2003, 39(4):139-145 | DOI: 10.17221/3833-PPS

Molecular variability of Citrus exocortis viroid in a single naturally infected citrus tree

Amine Elleuch, Mohamed Marrakchi, Dominique Lévesque, Nabina Bessais, Jean Pierre Perreault, Hatem Fakhfakh
Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie, Faculté des Sciences
1

We report the nucleotide sequence of Citrus exocortis viroid (CEVd) from a single natural infected citrus tree of the clementinier variety (Citrus clementina) in Tunisia. The sequence variability of this viroid from its natural host without using an alternative passage by an indicator host or an artificial inoculation was found to occur at 14 positions, giving a variability of 3.8%. This work confirms that naturally - occurring viroids belonging to the Pospiviroid genera contain a mixture of sequence variants. In addition, this study confirms the worldwide distribution of this viroid.

Keywords: nucleotide sequence; CEVd; genome; quasi-species

Published: December 31, 2003  Show citation

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Elleuch A, Marrakchi M, Lévesque D, Bessais N, Perreault JP, Fakhfakh H. Molecular variability of Citrus exocortis viroid in a single naturally infected citrus tree. Plant Protect. Sci. 2003;39(4):139-145. doi: 10.17221/3833-PPS.
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    References

    1. A K., G G., M A., D T B., P V. (2001): The molecular characterization of 16 new sequence variants of Hop stunt viroid reveals the existence of invariable regions and a conserved hammerhead-like structure on the viroid molecule. J. Gen. Virol., 82: 953-962. Go to original source... Go to PubMed...
    2. A S., H C., D J.C., F R. (1998): Genomic structure of three phenotypically different isolates of peach latent mosaic viroid: implications of the existence of constraints limiting the heterogeneity of viroid quasi-species. J. Virol., 72: 7397-7406. Go to original source... Go to PubMed...
    3. A M.A., F A., D J., M J.M., G -A F. (1997): Contribution of mutation and RNA recombination to the evolution of a plant pathogenic RNA. J. Mol. Evol., 44: 81-88. Go to original source... Go to PubMed...
    4. A L., L O., H R., B -J M. (1991): Nucleotide sequence of a new viroid species, citrus bent leaf viroid (CBLVd) isolated from grapefruit in Israel. Nucl. Acids Res., 19: 4767. Go to original source... Go to PubMed...
    5. D E., H J.J. (1994): Mutation rates and rapid evolution of RNA viruses. In: M S.S. (ed.): The Evolutionary Biology of Viruses. Raven Press, New York: 161-184.
    6. D -V N., R C.N., R -B R., S J.S. (1988): A definition of citrus viroid groups and their relationship to the exocortis disease. J. Gen. Virol., 69: 3069-3080. Go to original source...
    7. E M. (1993): The origin of genetic information: viruses as models. Gene, 135: 37-47. Go to original source... Go to PubMed...
    8. E A., F H., P M., L P., M M., P J.P. (2002): Sequencing of Australian grapevine viroid and yellow speckle viroid isolated from a Tunisian grapevine without passage in an indicator plant. Eur. J. Plant Pathol., 108: 815-820.
    9. F R., D -V N., P V., S J.S. (1985): Detection of viroid and viroid-like RNAs from grapevine. J. Gen. Virol., 66: 2095-2102. Go to original source...
    10. F R., R J.W., B -J M., D T.O. (2000): Viroids. In: R M.H.V., F C.M., B D.H.L., C E.B., E M.K., L S.M., M J., M M.A., M G D.J., P C.R., W R.B. (eds): Virus Taxonomy. 7th Rep. Int. Com. Taxon Viruses. San Diego, Academic Press: 1009-1024.
    11. G -S A., K A., C T., Z W. (1997): The genetic stability of potato spindle tuber viroid (PSTVd) molecular variants. RNA, 3: 68-74. Go to PubMed...
    12. G H.J., K G., D H., R M., J P., L C., A H., R K., S H.L. (1982): Nucleotides sequence and secondary structure of Citrus exocortis and Chrysanthemum stunt viroid. Eur. J. Biochem., 121: 249-257 Go to original source... Go to PubMed...
    13. I T., I H., O K., I T., N K., H T., I T., I M., K T. (2002): Multiple citrus viroids in citrus from Japan and their ability to produce exocortis-like symptoms in citron. Phytopathology, 92: 542-547. Go to original source... Go to PubMed...
    14. P M. (2003): SubViral RNA: a database of the smallest known auto-replicable RNA species. Nucl. Acids Res., 31: 444-445. Go to original source... Go to PubMed...
    15. P M., L D., O J., L S., L S., L J., T D.A., E K.C., S L.J., P J.P. (2000): Sequencing of peach latent mosaic viroid variants from nine north American peach cultivars shows that this RNA folds into a complex secondary structure. Virology, 271: 37-45. Go to original source... Go to PubMed...
    16. P H., R K., L R., H R., B -J M., S H.L. (1991): Primary and secondary structure of citrus viroid IV, a new chimeric viroid present in dawarfed grapefruit in Israel. Nucl. Acids Res., 19: 6640. Go to original source... Go to PubMed...
    17. R A.G., S J.A., A Z.S., S J.S. (1994): Nucleotide sequence and structural features of the group III citrus viroids. J. Gen. Virol., 75: 3581-3584. Go to original source... Go to PubMed...
    18. S J.S., W L.G. (1972): Exocortis virus: an infectious free - nucleic acid plant virus with unusual properties. Virology, 47: 456-466. Go to original source... Go to PubMed...
    19. S J.S., R C.N., R -B R., D -V N. (1988): Citrus cachexia viroid, a new viroid of citrus: relationship to viroids of the exocortis disease complexe. J. Gen. Virol., 69: 3059-3068. Go to original source...
    20. S J.S., S J.A., R A.G., S R.H. (1993): Isolates of citrus exocortis viroid recovered by host and tissue selection. J. Gen. Virol., 74: 2427-2436. Go to original source... Go to PubMed...
    21. S D., C M., S J.A., S J.S. (2001): CEVd-induced symptom modification as a response to a host-specific temperature-sensitive reaction. Virology, 280: 115-123. Go to original source... Go to PubMed...
    22. T J.D., H D.G., G T.J. (1994): The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl. Acids Res., 22: 4673-4680. Go to original source... Go to PubMed...
    23. V J.E., S R.H. (1985): Eleven new sequence variants of citrus exocortis viroid and the correlation of sequence with pathogenicity. Nucl. Acids Res., 13: 2907-2920. Go to original source... Go to PubMed...

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