Plant Protect. Sci., 2004, 40(3):101-106 | DOI: 10.17221/991-PPS

Regulation of metabolic pathways PVY-RNA biosynthesis in tobacco: glycolytic pathway

Luděk Šindelář, Milada Šindelářová
Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic

Tobacco plants infected with potato virus Y were studied at the stage of acute infection. Key enzymes of the glycolytic pathway, their regulation and the content of involved intermediates were monitored. The activities of the key enzymes of the glycolytic pathway (6-phosphofructokinase, fructosebisphosphatase and pyruvate kinase), determined in both crude homogenates and after partial purification, did not differ from the activities found in healthy control tissues. In virus-infected tissues the content of ATP was higher than in the healthy control. The levels of ADP and AMP decreased soon after inoculation, but increased at the end of the experimental period. The content of inorganic phosphate was not influenced by infection. No difference in adenylate energy charge was observed between healthy and virus-infected tissues. This implies that the rates of the glycolytic pathway in vivo are not altered during the acute stage of infection.

Keywords: ATP; ADP; AMP; fructosebisphosphatase; Nicotiana tabacum L. cv. Samsun; 6-phosphofructokinase; pyruvate kinase

Published: September 30, 2004  Show citation

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Šindelář L, Šindelářová M. Regulation of metabolic pathways PVY-RNA biosynthesis in tobacco: glycolytic pathway. Plant Protect. Sci. 2004;40(3):101-106. doi: 10.17221/991-PPS.
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