Plant Protect. Sci., 2007, 43(3):94-102 | DOI: 10.17221/2245-PPS

Influence of baculovirus AdorGV on the mortality of larvae and pupae of summer fruit tortrix Adoxophyes orana in laboratory conditions

Karel Peppernę
Czech University of Life Sciences Prague, Prague-Suchdol and Crop Research Institute, Prague-Ruzynì, Czech Republic

The mortality of larvae and pupae of Adoxophyes orana was examined by keeping larvae of each larval instar (L1-L5) on an artificial diet in laboratory conditions. Larvae were infected by using an artificial diet containing AdorGV-based CAPEX® 2. Samples of uninfected larvae from each instar served as controls. The mortality of larvae infected in the 1st instar was 100%, compared to a mortality of 68% in the control. In both, the larvae died before the 5th larval instar. With larvae infected in subsequent instars the mortality rate declined gradually (96%-72%-40%-12%) and death occurred predominantly in the 5th larval instar. The mortality of larvae in the controls was low (12%-12%-0%-0%). The mortality of pupae from larvae infected in the 2nd, 3rd and 4th instar was high (100%-86%-93%), and the mortality of larvae and pupae combined was close to 100%. Mortality of pupae developed from larvae infected in the 5th instar was 27% and that of larvae and pupae combined was 36%. The mortality of pupae developed from uninfected larvae in all controls was low (max. 8%). These results demonstrated the high efficacy of AdorGV to cause high mortality of larvae and pupae of Adoxophyes orana in laboratory conditions.

Keywords: summer fruit tortrix; Adoxophyes orana; AdorGV; mortality; larvae; pupae

Published: September 30, 2007  Show citation

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Peppernę K. Influence of baculovirus AdorGV on the mortality of larvae and pupae of summer fruit tortrix Adoxophyes orana in laboratory conditions. Plant Protect. Sci. 2007;43(3):94-102. doi: 10.17221/2245-PPS.
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References

  1. Aizawa K., Nakazato Y. (1963): Diagnosis of diseases in insects: 1959-1962. Musei, 37: 155-158.
  2. Andermatt M. (1991): Field efficacy and environmental persistence of the granulosis viruses of summer fruit tortrix, Adoxophyes orana. IOBC/WPRS Bulletin, 14: 73-74.
  3. Andermatt M. (2005): Available at http://www.biocontrol.ch/images/CAPEX®%20leaflet.pdf
  4. Ankersmit G.W., Rabbinge R., Dijkman H. (1977): Studies on the sterile-male technique as a means of control of Adoxophyes orana (Lepidoptera, Tortricidae). 4. Technical and economic aspects of mass-rearing. Netherland Journal of Pathology, 83: 27-39. Go to original source...
  5. Asano S. (2005): Ultraviolet protection of granulovirus product using iron oxide. Applied Entomology and Zoology, 40: 359-364. Go to original source...
  6. Blissard G.W., Rohrmann G.F. (1990): Baculovirus diversity and molecular biology. Annual Review of Entomology, 35: 127-155. Go to original source... Go to PubMed...
  7. Boucias D.G., Abbas M.S.T., Rathbone L., Hostetler N. (1987): Predators as potential dispersal agents of the nuclear polyhedrosis virus of Anticarsia gemmatalis (Lep.: Noctuidae) in soybean. Entomophaga, 32: 97-108. Go to original source...
  8. Charmilot P.J. (1992): Progress and prospects for selective means of controlling tortricid pests of orchards. Acta Phytopathologica et Entomologica Hungarica, 27: 165-176.
  9. Cunningham J.C., Knaupp W.J., Howse G.M. (1991): Development of nuclear polyhedrosis virus for control of gypsy moth (Lepidoptera: Lymantriidae) in Ontario. I. Aerial spray trials in 1998. Canadian Entomologist, 123: 601-609. Go to original source...
  10. Geest V.D., Evenhuis H.H. (1991): Tortricid Pests, their Biology, Natural Enemies and Control. Elsevier Science Publisher, Amsterdam.
  11. Fuxa J.R. (1987): Ecological considerations for the use of entomopathogens in IPM. Annual Review of Entomology, 32: 225-251. Go to original source...
  12. Ito Y., Shiga M., Oho N., Nakazawa H. (1977): A granulosis virus, possible biological agent for control of Adoxophyes orana (Lepidoptera, Tortricidae) in apple orchards. III. A preliminary model for population management. Researches on Population Ecology, 19: 33-50. Go to original source...
  13. Kabaluk T., Gazdik K. (2005): Directory of microbial pesticides for agricultural crops in OECD Countries. Available at http://www.agr.gc.ca/env/pdf/cat_e.pdf
  14. Kocourek F., Pultar O., Stará J. (2007): Comparison of the efficacy of AdorGV and chemical insecticides against summer fruit tortrix (Adoxophyes orana) in commercial apple orchards in the Czech Republic. OILB/WPRS Bulletin, 30: 171-176.
  15. Kundu J.K., Stará J., Kocourek F., Pultar O. (2003): Polymerase chain reaction assay for Cydia pomonella granulovirus detection in Cydia pomonella population. Acta Virologica, 47: 153-157. Go to PubMed...
  16. Luisier N., Benz G. (1994): Improving the efficiency of the granulosis virus of Adoxophyes orana F. v. R. for microbiological control. OILB/SROP Bulletin, 17: 273.
  17. Morgan D. (1991): The sensitivity of simulation models to temperature changes. OEPP/EPPO Bulletin, 21: 393-397. Go to original source...
  18. Moscardi F. (1999): Assessment of the application of baculoviruses for control of Lepidoptera. Annual Review of Entomology, 44: 257-289. Go to original source... Go to PubMed...
  19. Oho N. (1975): Possible utilization of a granolosis virus for control of Adoxophyes orana Fischer von Roeslerstamm (Lepidoptera: Tortricidae) in apple orchards. JIBP Synthesis (Tokio), 7: 61-68.
  20. Rohrmann G.F. (1992): Baculovirus structural proteins. Journal of General Virology, 73: 749-761. Go to original source... Go to PubMed...
  21. Sekita N. (1996): A granulosis virus as a control agent of Adoxophyes orana fasciata Walshingam (Lepidoptera: Tortricidae) in apple orchards. FFTC Book Series, 47: 125-130.
  22. Shiga M., Yamada H., Oho N., Nakazawa H., Ito Y. (1973): A granulosis virus, possible biological agent for control of Adoxophyes orana (Lepidoptera: Tortricidae) in apple orchards. II. Semipersistent effect of artificial dissemination into an apple orchard. Journal of Invertebrate Pathology, 21: 149-157. Go to original source...
  23. Stará J., Bohdanecká D., Kocourek F., Pultar O., Kundu J.K. (2007): Evaluation of biological efficacy of Adoxophyes orana granulovirus on the reduction of Adoxophyes orana population by PCR detection. OILB/WPRS Bulletin 30: 177-180.
  24. Winstanley D., O'Reilly D.R. (1999): Granuloviruses. In: Webster R., Granoff A. (eds): The Encyclopedia of Virology. Vol. 1. 2nd Ed. Academic Press, London: 140-146. Go to original source...
  25. Yamada H., Oho N. (1973): A granulosis virus, possible biological agent for control of Adoxophyes orana (Lepidoptera: Tortricidae) in apple orchards. I. Mass production. Journal of Invertebrate Pathology, 21: 144-148. Go to original source...

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