Plant Protect. Sci., 2007, 43(4):142-145 | DOI: 10.17221/2243-PPS

Contribution to identify the causal gents of Dutch elm disease in the Czech Republic

Miloň Dvořák1, Michal Tomšovský1, Libor Jankovský1, David Novotný2
1 Department of Forest Protection and Game Management, Faculty of Forestry and Wood Technology, Mendel University of Agriculture and Forestry in Brno, Brno, Czech Republic
2 Department of Mycology, Division of Plant Medicine, Crop Research Institute, Prague-Ruzyně, Czech Republic

This study provides new data on Dutch elm disease in the Czech Republic. Ophiostoma novo-ulmi is reported for the first time in the area of the Czech Republic, as well as both subspecies ssp. novo-ulmi (indigenous in the area of the Ukraine and Moldavia), and ssp. Americana indigenous in North America. The majority of the recorded strains belonged to O. n.-u. ssp. novo-ulmi, while O. n.-u. ssp. Americana and hybrids of these two subspecies were found less frequently. On the other hand, Ophiostoma ulmi was not found at all in the investigated samples. Identification on the subspecies level was performed by methods of molecular biology, i.e. PCR and RFLP of gene regions cu and col1.

Keywords: Ulmus; Ophiostoma novo-ulmi; PCR; RFLP

Published: December 31, 2007  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Dvořák M, Tomšovský M, Jankovský L, Novotný D. Contribution to identify the causal gents of Dutch elm disease in the Czech Republic. Plant Protect. Sci. 2007;43(4):142-145. doi: 10.17221/2243-PPS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Brasier C.M. (1979): Dual origin of recent Dutch elm disease outbreaks in Europe. Nature, 281: 78-79. Go to original source...
    2. Brasier C.M. (1983): A cytoplasmically transmitted disease of Ceratocystis ulmi. Nature, 305: 220-223. Go to original source...
    3. Brasier C.M. (1991): O. novo-ulmi sp. nov., causative agent of current Dutch elm disease pandemics. Mycopathologia, 115: 151-161. Go to original source...
    4. Brasier C.M., Kirk S.A. (2001): Designation of the EAN and NAN races of Ophiostoma novo-ulmi as subspecies. Mycology Research, 105: 547-554. Go to original source...
    5. Gibbs J.N., Brasier C.M. (1973): Correlation between cultural characters and pathogenicity of Ceratocystis ulmi from Europe and North America. Nature, 241: 381-383. Go to original source...
    6. Gremmen J., Heybroek H.M., Kam M. de (1976): The aggressive strain of Ceratocystis ulmi in the Netherlands. Nederlands Bosbouw Tijdschrift, 48: 137-143.
    7. Hoegger P.J., Binz T., Heiniger U. (1996): Detection of genetic variation between Ophiostoma ulmi and the NAN and EAN races of O. novo-ulmi in Switzerland using RAPD markers. European Journal of Forestry Pathology, 26: 57-68. Go to original source...
    8. Jančařík V. (1999): Grafióza jilmů. Epidemie pokračuje nebo nastává její útlum. Silva Bohemica, 9: 7-10.
    9. Kalandra A., Pfeffer A. (1935): Příspěvek ke studiu graphiosy na jilmech. Lesnická práce, 14: 1-14.
    10. Konrad H., Kirisits T., Riegler M., Halmschlager E., Stauffer C. (2002): Genetic evidence for natural hybridization between the Dutch elm disease pathogens Ophiostoma novo-ulmi ssp. novo-ulmi and O. novo-ulmi ssp. americana. Plant Pathology, 51: 78-84. Go to original source...
    11. Pipe N.D., Buck K.W., Brasier C.M. (1997): Comparison of the cerato-ulmi (cu) gene sequences of the Himalayan Dutch elm disease fungus Ophiostoma himal-ulmi with those of O. ulmi and Ophiostoma novo-ulmi suggests that the cu gene of O. novo-ulmi is unlikely to have been acquired recently from O. himalulmi. Mycology Research, 101: 415-421. Go to original source...
    12. Polák O. (1932): Ohrožení našich jilmů houbou Graphium ulmi. Referát o přednášce prof. Pekla. Československý les, 12: 87-89.

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content