Plant Protect. Sci., 2008, 44(3):81-84 | DOI: 10.17221/28/2008-PPS

Electron microscopy of structures present in embryonic cells of plants infected with Plum pox virus

Jaroslav Polák, Milan Jokeš, Miloslava Ducháčová, Alena Hauptmanová, Petr Komínek
Department of Virology, Division of Plant Health, Crop Research Institute, Prague-Ruzyně, Czech Republic

Electron microscopy was used to detect the presence of virus particles or inclusions in growth tips and parenchymatic cells of leaves of plum, apricot and peach trees artificially infected with Plum pox virus (PPV). Typical pinwheels were found in ultrathin sections of leaves of PPV infected plums, apricots and peaches. Filamentous particles or their aggregates approximately 750 nm long were found in ultrathin sections of growth tips of plum, apricot, and peach shoots with a diameter of 0.5 mm. Pinwheels were never present in embryonic cells. No virus particles were found in ultrathin sections of growth tips of PPV infected plum, apricot and peach shoots of 0.2 mm in diameter. Embryonic cells of growth tips up to 0.2 mm in diameter are PPV free. PPV particles are present in growth tips at a distance 0.2-0.5 mm from the top; the virus is probably multiplied in this part of the growth tips.

Keywords: sharka; herbaceous and woody hosts; meristematic cells; growth tips; PPV aggregates; ultrathin sections

Published: September 30, 2008  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Polák J, Jokeš M, Ducháčová M, Hauptmanová A, Komínek P. Electron microscopy of structures present in embryonic cells of plants infected with Plum pox virus . Plant Protect. Sci. 2008;44(3):81-84. doi: 10.17221/28/2008-PPS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Abdel-Ghaffar M.H., Abo El-Nasr M.A., Hari V. (1998): Studies on an apricot strain of Plum pox Potyvirus isolated from El Amar, Egypt. Acta Horticulturae, 472: 385-392. Go to original source...
    2. Bovey R. (1971): Etude au microscope electronique de quelques alterations de structure produities par le virus de la sharka (Plum pox virus) dans les cellulas infectees. Annales de Phytopathologie, 3: 225-232.
    3. Edwardson J.R. (1974): Some properties of the potato virus Y group. Florida Agricultural Experimental Station Monograph Series, 4.
    4. Edwardson J.R., Christie R.G., Ko N.J. (1984): Potyvirus cylindrical inclusions - subdivision - IV. Phytopathology, 74: 1111-1114. Go to original source...
    5. Golinowski W., Garbaczewska G. (1980): Cytological changes of plants infected with Plum pox virus. Acta Phytopathologica Academiae Scientiarum Hungaricae, 15: 315-318. Go to original source...
    6. Kassanis B. (1939): Intranuclear inclusions in virus infected plants. Annals of Applied Biology, 26: 705-709. Go to original source...
    7. Kunkel L.O. (1922): Amoeboid bodies associated with Hippeastrum mosaic. Science, 55: 73. Go to original source... Go to PubMed...
    8. Macovei A. (1971): Electron microscopic evidence of pinwheel structures in Nicotiana clevelandii - D cells infected with plum pox virus. Revue Roumaine de Biologie (Series Botanique), 16: 331-334.
    9. Martelli G.P., Russo M. (1977): Plant virus inclusion bodies. Advances in Virus Research, 21: 175-266. Go to original source... Go to PubMed...
    10. Pleše N., Rilović M., Weischer M. (1969): Novi domadari i intracelularne inkluzije virus Sarke. Zaštita Bilja, 104: 143-150. Go to original source...
    11. Wetzel T., Candresse T., Ravelonandro M., Dunez J. (1991): A polymerase chain reaction adapted to plum pox virus detection. Journal of Virological Methods, 33: 355-366. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content