Plant Protect. Sci., 2011, 47(1):5-12 | DOI: 10.17221/16/2010-PPS

Sensitivity to fungicides in the isolates of Phytophthora infestans (Mont.) de Bary in the Czech Republic from 2003 to 2008

Jana Mazáková1, Miloslav Zouhar1, Pavel Ryšánek1, Vladimír Táborský1, Ervín Hausvater2, Petr Doležal2
1 Department of Plant Protection, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences in Prague, Prague-Suchdol, Czech Republic
2 Department of Protection, Potato Research Institute in Havlíčkův Brod, Ltd., Havlíčkův Brod, Czech Republic

In the growing seasons from 2003 to 2008, 547 isolates of Phytophthora infestans from five regions in the Czech Republic were collected and examined for their sensitivity to the active ingredients (metalaxyl, dimethomorph and propamocarb-HCl) of frequently used fungicides. The response of the isolates to each of these substances was examined using the in vitro amended-agar method; in 352 of these isolates, the sensitivity to metalaxyl was also assessed by the floating leaf-disc assay. The majority of the isolates were sensitive (89.8%) to metalaxyl. Resistant isolates were found only in two of the sample years (2003 and 2008); they represented 58% of the samples in 2003 and only 29% in 2008. Four isolates from 2004 were found to be intermediate for their level of resistance. All the isolates that were tested were sensitive to dimethomorph and propamocarb-HCl; these particular substances completely suppressed mycelial growth at 1 µg a.i. per ml.

Keywords: late blight; fungicide resistance; metalaxyl; dimethomorph; propamocarb-hydrochloride

Published: March 31, 2011  Show citation

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Mazáková J, Zouhar M, Ryšánek P, Táborský V, Hausvater E, Doležal P. Sensitivity to fungicides in the isolates of Phytophthora infestans (Mont.) de Bary in the Czech Republic from 2003 to 2008. Plant Protect. Sci. 2011;47(1):5-12. doi: 10.17221/16/2010-PPS.
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    References

    1. Albert G., Curtze J., Drandarevski Ch.A. (1988): Dimethomorph (CME 151), a novel curative fungicide. Proceedings of British Crop Protection Conference. Pest and Diseases, Vol. 1: 17-24.
    2. Anonymous (2009): Available at http://www.eucablight.org/Pathogen/Pathogen.asp?FileName=GenotypeAnalysis. asp (accessed 11.08. 2009)
    3. Bagirova S.F., An Zsan Li, Dolgova A.V., Elansky S.N., Shaw D.S., Dyakov Y.T. (2001): Mutants of Phytophthora infestans resistant to dimethomorph fungicide. Journal of Russian Phytopathological Society, 2: 19-24.
    4. Brent K.J., Hollomon D.W. (2007): Fungicide resistance in crop pathogens: How can it be managed? FRAC Monograph No. 1. 2nd Rev. Ed. Fungicide Resistance Action Committee, Croplife International, Brussels.
    5. Carlisle D.J., Cooke L.R., Brown A.E. (2001): Phenotypic and genotypic characterisation of northern Ireland isolates of Phytophthora infestans. European Journal of Plant Pathology, 107: 291-303. Go to original source...
    6. Cohen Y., Baider A., Cohen B.H. (1995): Dimethomorph activity against oomycete fungal plant pathogens. Phytopathology, 85: 1500-1506. Go to original source...
    7. Cooke D.E.L., Young V., Birch P.R.J., Toth R., Gourlay F., Day J.P., Carnegie S.F., Duncan J.M. (2003): Phenotypic and genotypic diversity of Phytophthora infestans populations in Scotland (1995-97). Plant Pathology, 52: 181-192. Go to original source...
    8. Cooke D.E.L., Lees A.K., Grønbech Hansen J., Lassen P., Andersson B., Bakonyi J. (2009): From a European to a global database of Phytophthora infestans genetic diversity: examining the nature and significance of population change. Acta Horticulture (ISHS), 834: 19-26. Go to original source...
    9. Daggett S.S., Götz E., Therrien C.D. (1993): Phenotypic changes in populations of Phytophthora infestans from eastern Germany. Phytopathology, 83: 319-323. Go to original source...
    10. Davidse L.C., Looijen D., Turkensteen L.J., Van der Wal D. (1981): Occurrence of metalaxyl-resistant strains of Phytophthora infestans in Dutch potato fields. Netherlands Journal of Plant Pathology, 87: 65-68. Go to original source...
    11. Day J.P., Wattier R.A.M., Shaw D.S., Shattock R.C. (2004): Phenotypic and genotypic diversity in Phytophthora infestans on potato in Great Britain, 1995-1998. Plant Pathology, 53: 303-315. Go to original source...
    12. Dowley L.J., O'Sullivan E. (1981): Metalaxyl-resistant strains of Phytophthora infestans (Mont.) de Bary in Ireland. Potato Research, 24: 417-421. Go to original source...
    13. Elansky S.N., Apryshko V.P., Milyutina D.I., Kozlovsky B.E. (2007): Resistance of Russian strains of Phytophthora infestans to fungicides Metalaxyl and Dimethomorph. Moscow University Biological Sciences Bulletin, 62: 11-14. Go to original source...
    14. Gisi U. (2002): Chemical control of downy mildews. In: Spencer-Phillips P.T.N., Gisi U., Lebeda A.: Advances in Downy Mildew Research. Kluwer Academic Publishers, Dordrecht: 119-159. Go to original source...
    15. Gisi U., Cohen Y. (1996): Resistance to phenylamide fungicides: a case study with Phytophthora infestans involving mating type and race structure. Annual Review of Phytopathology, 43: 549-572. Go to original source... Go to PubMed...
    16. Goodwin S.B., Spielman L.J., Matusz ak J.M., Bergeron S.N., Fry W.E. (1992): Clonal diversity and genetic differentiation of Phytophthora infestans populations in northern and central Mexico. Phytopathology, 82: 955-961. Go to original source...
    17. Goodwin S.B., Cohen B.A., Fry W.E. (1994): Panglobal distribution of a single clonal lineage of the Irish potato famine fungus. Proceedings of the National Academy of Science of the United States of America, 91: 11591-11595. Go to original source... Go to PubMed...
    18. Goodwin S.B., Sujkowski L.S., Fry W.E. (1996): Widespread distribution and probable origin of resistance to metalaxyl in clonal genotypes of Phytophthora infestans in the United States and western Canada. Phytopathology, 86: 793-800. Go to original source...
    19. Goodwin S.B., Smart C.D., Sandrock R.W., Deahl K.L., Punja Z.K., Fry W.E. (1998): Genetic change within populations of Phytophthora infestans in the United States and Canada during 1994 to 1996: Role of migration and recombination. Phytopathology, 88: 939-949. Go to original source... Go to PubMed...
    20. Hausvater E., Rasocha V. (2000): Nové aspekty plísně bramborové a aktualizace integrované ochrany. [Závěrečná zpráva.] Výzkumný ústav bramborářský Havlíčkův Brod, s.r.o., Havlíčkův Brod.
    21. Hermansen A., Hannukkala A., Naerstad R.H., Brurberg M.B. (2000): Variation in populations of Phytophthora infestans in Finland and Norway: mating type, metalaxyl resistance and virulence phenotype. Plant Pathology, 49: 11-22. Go to original source...
    22. Hohl H.R., Iselin K. (1984): Strains of Phytophthora infestans from Switzerland with A2 mating type behaviour. Transactions of the British Mycological Society, 83: 529-530. Go to original source...
    23. Lehtinen A., Hannukkala A., Andersson B., Hermansen A., Le V.H., Naerstad R., Brurberg M.B., Nielsen B.J., Hansen J.G., Yuen J. (2008): Phenotypic variation in Nordic populations of Phytophthora infestans in 2003. Plant Pathology, 57: 227-234.
    24. Möller K., Dilger M., Habermeyer J., Zinkernagel V., Flier W. G., Hausladen H. (2009): Population studies on Phytophthora infestans on potatoes and tomatoes in southern Germany. European Journal of Plant Pathology, 124: 659-672. Go to original source...
    25. Peters R.D., Platt H.W., Hall R. (1998): Characterization of changes in populations of Phytophthora infestans in Canada using mating types and metalaxyl sensitivity markers. Canadian Journal of Plant Pathology, 20: 259-273. Go to original source...
    26. Reis A., Ribeiro F.H.S., Maffia L.A., Mizubuti E.S.G. (2005): Sensitivity of Brazilian isolates of Phytophthora infestans to commonly used fungicides in tomato and potato crops. Plant Disease, 89: 1279-1284. Go to original source... Go to PubMed...
    27. Runno E., Koppel M. (2006): The question of metalaxyl resistance on late blight fungus in Estonia. Agronomy Research, 4: 341-344.
    28. Shattock R.C. (1988): Studies on the inheritance of resistance to metalaxyl in Phytophthora infestans. Plant Pathology, 37: 4-11. Go to original source...
    29. Śliwka J., Sobkowiak S., Lebecka R., AvendañoCórcoles J., Zimnoch-Guzowska E. (2006): Mating type, virulence, aggressiveness and metalaxyl resistance of isolates of Phytophthora infestans in Poland. Potato Research, 49: 155-166. Go to original source...
    30. Sozzi D., Staub T. (1987): Accuracy of methods to monitor sensitivity of Phytophtlora infestans to phenylamide fungicides. Plant Disease, 71: 422-425. Go to original source...
    31. Stein J.M., Kirk W.W. (2004): The generation and quantification of resistance to dimethomorph in Phytophthora infestans. Plant Disease, 88: 930-934. Go to original source... Go to PubMed...
    32. Urban J., Lebeda A. (2007): Variation of fungicide resistance in Czech populations of Pseudoperonospora cubensis. Journal of Phytopathology, 155: 143-151. Go to original source...
    33. Zhu X.Q., Che X.B., Guo L.Y., Wang Y.H. (2004): Mating type of Phytophthora infestans from six provinces (cities) in China and their sensitivity to several fungicides. Plant Protection, 30(4): 20-23. Recived for publication February 23, 2010

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