Plant Protect. Sci., 2011, 47(4):157-165 | DOI: 10.17221/72/2010-PPS

Effect of storage on redgram (Cajanus cajan /L./ Millsp) and greengram (Vigna radiata /L./ Wilczek) with particular reference to lipid composition

Mysore Ranganayaka Gopinath1, Kari Sambiah2, Siddapura Ramachandra Niranjana1
1 Department of Studies in Applied Botany &
Biotechnology, University of Mysore, Manasagangotri, Mysore, Karnataka, India
2 Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Mysore, Karnataka, India

The effects of storage on the lipid composition of three redgram (TTB-7, BRG-1, and ICP-8863) and three greengram (PUSA BISAKI, KDM-1, and CHINA MOONG) varieties were studied. Environment variables such as temperature and relative humidity were recorded during storage period. Moisture content was found to increase along with the percent incidence of storage insects. The incidence of storage moulds and insects was recorded in all the samples; results showed that storage moulds belonged to Aspergillus species and the insect Callosobruchus chinensis infested the stored samples. The total fat, triglycerides, phospholipids, free fatty acids and peroxide value were evaluated in the control and in samples stored for three and six months. Results showed that storage depleted total fat (1.94-1.75 g), triglycerides (1.46-1.07 g), whereas phospholipids (0.06-0.21g), free fatty acids (0.002-0.01 g) and peroxide values (2.14-4.46 meq) increased. The fatty acid content of palmitic (26.03-23.56%), stearic (7.4-5.46%), linoleic (56.2-45.2%) and linolenic acids (6.9-4.7%) decreased, but oleic acid content increased (8.3-21.6%) in all the varieties during storage.

Keywords: redgram; greengram; triglycerides; phospholipids; peroxide value; insects; moulds

Published: December 31, 2011  Show citation

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Gopinath MR, Sambiah K, Niranjana SR. Effect of storage on redgram (Cajanus cajan /L./ Millsp) and greengram (Vigna radiata /L./ Wilczek) with particular reference to lipid composition. Plant Protect. Sci. 2011;47(4):157-165. doi: 10.17221/72/2010-PPS.
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    References

    1. Anonymous (1996): International Rules for Seed Testing Association. Seed Science and Technology, 29: 335.
    2. Anonymous (2003): International Rules for Seed Testing. Moisture Content Determination. International Seed Testing Association, Chapter 9: 3-4.
    3. Bernal Lugo I., Leopold A.C. (1992): Changes in soluble carbohydrates during seed storage. Physiologia Plantarum, 98: 1207-1210. Go to original source... Go to PubMed...
    4. Bhattacharya K., Raha S. (2002): Deteriorative changes of maize, groundnut and soybean seeds by fungi storage. Mycopathologia, 55: 135-141. Go to original source... Go to PubMed...
    5. Buck A.L. (1981): New equations for computing vapor pressure and enhancement factor. Journal of Applied Meterology, 20: 1527-1532. Go to original source...
    6. Burkert T.F., Maugeri F.M., Rodrigues M.I. (2004): Optimization of extracelluar lipases production by Geotrichum sp. using factorial design. Bioresources Technology, 910: 77-84. Go to original source... Go to PubMed...
    7. Chaiyasit W., Elias R.J., McClements D.J., Decker E.A. (2007): Role of physical structures in bulk oils on lipid oxidation. Critical Reviews in Food Science and Nutrition, 47: 299-317. Go to original source... Go to PubMed...
    8. Dhingra O.D., Jham G.N., Rodrigues F.A., Silva G.J. jr., Costa M.L.N. (2009): Fumigation with essential oil of mustard retards fungal growth and accumulation of ergosterol and free fatty acid in stored shelled groundnuts. Journal of Stored Products Research, 45: 24-31. Go to original source...
    9. Doworth C., Christensen C.M. (1968): Influence of moisture content and storage time upon changes in the fungus flora, germinability and fat acidity values of soybean. Phytopathology, 58: 1457-1459.
    10. Fastnaught C.E., Berglund P.T., Dudgeon A.L., Hadley M. (2006): lipid changes during storage of milled hulless barley products. Cereal Chemistry, 83: 424-427. Go to original source...
    11. Fletcher M.J. (1968): A colorimetric method for estimating serum triglycerides. Clinica Chimica Acta, 22: 393-397. Go to original source... Go to PubMed...
    12. Goodman J.J. (1950): Adaptive production of amylase and lipase by three species of fungi. Science, 112: 176-179. Go to original source... Go to PubMed...
    13. Kakde R.B., Chavan A.M. (2011): Extracelluar lipase enzyme production by seed-borne fungi under the influence of physical factors. International Journal of Biology, 3: 94-100. Go to original source...
    14. Kamimur a E.S., Medieta O., Rodrigues M.I., Maugeri F. (2001): Studies on lipase-affinity adsorption using response-surface analysis. Biotechnology and Applied Biochemistry, 33: 153-159. Go to original source... Go to PubMed...
    15. Lisker N.A., Ben-Efraim A., Henis Y. (1985): Fungi growing on stored soybeans and their significance in lipid break down. Annals of Applied Biology, 107:117-126. Go to original source...
    16. Lapčík O., Hill M., Černý I., Lachman J., Al-Maharik N., Adlercreutz H., Hampl R. (1999): Immunoanalysis of isoflavonids in Pisum sativum and Vigna radiata. Plant Science, 148: 111-119. Go to original source...
    17. Mbata G.N. (1986). The susceptibility of variety of groundnuts to infestation by Tribolium castaneum during storage. Tropical Science, 26: 187-194.
    18. Mbata G.N. Osuji F.N. (1983): Some aspects of the biology of Plodia interpuctella (Huber) (Lepidoptera: Pfryalidae), a pest of stored groundnuts in Nigeria. Journal of Stored Product Research, 19: 141-151. Go to original source...
    19. Millner M., Geddes W.F. (1946a): Grain storage studies. III. The relationship between moisture content, mold growth and respiration of soybeans. Cereal Chemistry, 23: 225-247.
    20. Millner M., Geddes W.F. (1946b): Grain storage studies. IV. Biological and chemical factors involved in spontaneous heating of soybeans. Cereal Chemistry, 23: 449-470.
    21. Modgil R., Mehta U. (1996): Effect of graded levels of insect infestation on the chemical composition of bengalgram. Journal of Food Science and Technology, 33: 393-396.
    22. Morrison W.R., Smith L.M. (1964): Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron fluoride-methanol. Journal of Lipid Research, 5: 600-608. Go to original source... Go to PubMed...
    23. Ojimelukwe P.C. (2002): Changes induced by infestation on some chemical properties of cowpea seeds. Plant Foods for Human Nutrition, 57: 129-140. Go to original source... Go to PubMed...
    24. Ojimelukwe P.C., Onweluzo J.C., Okechukwu E. (1999): Effects of infestation on the nutrient content and physicochemical properties of two cowpea varieties. Plant Food for Human Nutrition, 53: 321-332. Go to original source... Go to PubMed...
    25. Patro K.C., Sahoo P. (2001): Effect of different levels of Callosobruchus chinensis (L.) infestation on deterioration pigeon pea and greengram seeds. Legume Research, 24: 109-111.
    26. Pearce R.S., Abdel Samad I.M. (1980): Change in fatty acid content of polar lipids during ageing of seeds of peanut (Arachis hypogea L.). Journal of Experimental Botany, 31: 1283-1290. Go to original source...
    27. Pitt J.I., Hocking A.D. (eds) (2009): Fungi and Food Spoilage. 3rd Ed. Springer, New York: 417 Go to original source...
    28. Priestly D.A., Leopold A.C. (1983a): Lipid changes during natural aging of soybean seeds. Physiologia Plantarum, 59: 467-470. Go to original source...
    29. Priestly D.A., Leopold A.C. (1983b): Lipid changes during natural aging of soybean seeds. Physiologia Plantarum, 59: 467-470. Go to original source...
    30. Sharma S., Gambir S., Munshi S.K. (2007): Changes in lipid and carbohydrates composition of germinating soybean seeds under different storage conditions. Asian Journal of Plant Sciences, 6: 502-507. Go to original source...
    31. Stewart J.C.M. (1980): Colorimetric determination of phospholipids with ammonium ferrothiocyanate. Analytical Biochemistry, 104: 10-14. Go to original source... Go to PubMed...
    32. Stine C.M., Harland H.A., Coulter S.T., Jenness R.A. (1953): Modified peroxide test for peroxide test for detection of lipid oxidation in dairy products. Journal of Dairy Research, 37: 202-208. Go to original source...
    33. Tripathy M.K., Sahoo Das B.C., Mohanty S. (2001): Efficacy of botanicals oils, plant powders and extracts against Callosobruchus chinensis (Linn) attacking blackgram. Legume Research, 24: 82-86.
    34. Vick B.A., Zimmerman D.C. (1976): Lipoxygenase and hydroperoxide lyase in germination in watermelon seedlings. Plant Physiology, 57: 780-788. Go to original source... Go to PubMed...

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