Plant Protect. Sci., 2011, 47(4):166-175 | DOI: 10.17221/3/2011-PPS

Toxicity and biochemical impacts of some new insecticide mixtures on cotton leafworm Spodoptera littoralis (Boisd.)

Ahmed E. M. ABD EL-MAGEED1, Shehata E. M. SHALABY2
1 Plant Protection Research Institute, Agricultural Research Centre, Dokki, Giza, Egypt
2 Pests & Plant Protection Department, National Research Centre, Dokki, Giza, Egypt

The susceptibility to five new insecticide mixtures: chlorosan, feroban, cygron, engeo, and kingbo was studied in the 2nd and 4th instar larvae of the cotton leafworm Spodoptera littoralis (Boisd.). The efficiency and residual effects of these compounds against S. littoralis under field conditions were also investigated. Obtained results revealed that feroban was the most effective compared with the other toxicants, while engeo was the least toxic insecticide in both instars after 2 and 5 days from treatment. Data also indicated that feroban had the longest half-life (Lt50) while engeo recorded the shortest one. Biochemical analysis showed that the tested compounds caused pronounced changes in acetyl cholinesterase and phenol oxidase.

Keywords: insecticide mixtures; toxicity; enzymes; Spodoptera littoralis

Published: December 31, 2011  Show citation

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ABD EL-MAGEED AEM, SHALABY SEM. Toxicity and biochemical impacts of some new insecticide mixtures on cotton leafworm Spodoptera littoralis (Boisd.). Plant Protect. Sci. 2011;47(4):166-175. doi: 10.17221/3/2011-PPS.
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    References

    1. Abbott W.S. (1925): A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, 18: 265-267. Go to original source...
    2. Abd El-Mageed A.E.M. (2002): Toxicological and analytical studies on some insecticides. [PhD Thesis.] Faculty of Agriculture, Mansoura University.
    3. Abd El-Mageed A.E.M., Anwar E.M., Elgohary L.R.A. (2008): Biochemical side effects for some commercial biocides on cotton leafworm. Archives of Phytopathology and Plant Protection, 41: 227-232. Go to original source...
    4. Abdel-Hafez M.M., Shaaban M.N., El-Malla, M.A., Farag M., Abdel-Kawy A.M. (1988): Effect of insect growth regulators on the activity transaminases with reference to protein and amino acids in the Egyptian cotton leafworm Spodoptera littoralis (Boisd.). Minia Journal of Agricultural Research and Development, 10: 1357-1372.
    5. Abdel-Hafez M.M., Mohanna A., Afifi M.A., Eid A.H. (1993): Effect of IGR/insecticide mixtures on esterases activity of Spodoptera littoralis. Journal of Product & Development, 1, 153-164.
    6. All J.N., Ali M., Ho'rnyak E.P., Weaver J.B. (1977): Joint action of two pyrethroids with methyl-parathion, methomyl, and chlorpyrifos on Heliothis zea and H. virescens in the laboratory and in cotton and sweetcorn. Journal of Economic Entomology, 70: 813-817. Go to original source...
    7. Anonymous (1991): EU Council Regulation (EEC) No 209/91, Annex II. Use of this product for organic EU certification may be recognized by the inspection body. Cert. No. BDOCA 148 Pr.
    8. Ascher K.R.S., Eliahu M., Ishaaya I., Zur M., BenMoshe E. (1986): Synergism of pyrethroid-organophosphorus insecticide mixtures in insects and their toxicity against Spodoptera littoralis larvae. Phytoparasitica, 14: 101-111. Go to original source...
    9. Aydin H., Gürkan M.O. (2006): The efficacy of spinosad on different strains of Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae). Turkish Journal of Biology, 30: 5-9.
    10. Bradford M.M. (1976): A rapid and sensitive method for the quatitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72: 248-254. Go to original source...
    11. Bynum E.D., Archer T.L., Plapp F.W. (1997): Comparison of banks Grass Mite and Two-spotted Spider Mite (Acari: Tetranychidae): responses to insecticides alone and synergistic combinations. Journal of Economic Entomology, 90: 1125-1130. Go to original source...
    12. Casida J.E., Ruzo L.O. (1980): Metabolic chemistry of pyrethroid insecticides. Pesticide Science, 11: 257-269. Go to original source...
    13. Corbel V., Chandre F., Darriet F., Lardeux F., Hougard J.M. (2003): Synergism between permethrin and propoxur against Culex quinquefasciatus mosquito larvae. Medical and Veterinary Entomology, 17: 158-164. Go to original source... Go to PubMed...
    14. Corbel V., Stankiewicz M., Bonnet J., Grolleau F., Hougard J.M., Lapied B. (2006): Synergism between insecticides permethrin and propoxur occurs through activation of presynaptic muscarinic negative feedback of acetylcholine release in the insect central nervous system. Neuro Toxicology, 27: 508-519. Go to original source... Go to PubMed...
    15. El-Aswad A.F. (2007): Efficiency of certain insecticides and insect growth regulators alone or in mixture with chlorpyrifos for the integrated control of the Egyptian cotton leafworm. Journal of Pest Control and Environmental Sciences, 15 (2): 29-48.
    16. El-Defrawi M.E., Toppozada A., Mansour N., Zeid M. (1964): Toxicological studies on Egyptian cotton leafworm Prodenia litura (F.). I. Suceptibility of different larval instar to insecticides. Journal of Economic Entomology, 57: 591-593. Go to original source...
    17. El-Saidy M.F., Auda M., Degheele D. (1990): Esterases and resistance to organophosphorus insecticides in Spodoptera littoralis (Boisd.). Mededelingen van de Faculteit Landbouwwetenschappen, Rijksuniversiteit Gent, 55: 565-576.
    18. Finney D.J. (1971): Probit analysis. A Statistical Treatment of the Sigmoid Response Curve. 7th Ed. Cambridge University Press, Cambridge.
    19. Gunning R.V., Moores G.D., Devonshire A.L. (1999): Esterase inhibitors synergise the toxicity of pyrethroids in Australian Helicoverpa armigera (Lepidoptera: Noctuidae). Pesticide Biochemistry and Physiology, 63: 52-62. Go to original source...
    20. Ishaaya I., Casida J.E. (1974): Dietary TH 6040 alters composition and enzyme activity of housefly larval cuticle. Pesticide Biochemistry and Physiology, 4: 484-490. Go to original source...
    21. Ishaaya I., Swirski E. (1976): Trehalase, invertase and amylase activities in the black scale Saissetia oleae and their relation to host adaptability. Journal of Insect Physiology, 22: 1025-1029. Go to original source...
    22. Ishaaya I., Ascher K.R.S, Casida J.E. (1983): Pyrethroid synergism by esterase inhibition in Spodoptera littoralis (Boisduval) larvae. Crop Protection, 2: 335-343. Go to original source...
    23. Ishaaya I., Mendelson Z., Ascher K.R.S., Casida J.E. (1985): Mixtures of synthetic pyrethroids and organophosphorus compounds for controlling the whitefly, Bemisia tabaci. Phytoparasitica, 13: 76-77.
    24. Kassem SH.M.I., Sherby S.M., Aly M.I., Zeid M.I., El-Sebae A.H. (1986): Studies on toxic effects of endotoxin, chitin synthesis inhibitor and mixtures of diflubenzouron with different insecticides on Egyptian cotton leafworm Spodoptera littoralis (Boisd.). Alexandrian Journal of Agricultural Research, 31: 327-336.
    25. Kulkrani A.P., Hodgson E. (1980): Metabolism of insecticides by mixed function oxidase systems. Pharmacology & Therapeutics, 8: 379-475. Go to original source... Go to PubMed...
    26. Martin T., Ochou O.G., Vaissayre M., Fournier D. (2003): Organophosphorous insecticides synergise pyrethroids in the resistant strain of cotton bollworm, Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) from West Africa. Journal of Economic Entomology, 96: 468-474. Go to original source... Go to PubMed...
    27. Motoyama N., Dauterman W.C. (1974): The role of non-oxidative metabolism in organophosphorus resistance. Journal of Agricultural and Food Chemistry, 22: 350-356. Go to original source... Go to PubMed...
    28. Mushtaq A. (2004): Potentiation/antagonism of deltamethrin and cypermethrins with organophosphate insecticides in the cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae). Pesticide Biochemistry and Physiology, 80: 31-42. Go to original source...
    29. Ozaki K., Sasaki Y., Kassai T. (1984): The insecticidal activity of mixtures of pyrethroids and organophosphates or carbamates against the insecticide-resistant green rice leafhopper, Nephotettix cincticeps Uhler. Journal of Pesticide Science, 9: 67-72. Go to original source...
    30. Powell M.E.A., Smith M.J.H. (1954): The determination of serum acid and alkaline phosphatases activity with 4-amino antipyrine. Journal of Clinical Pathology, 7: 245-248. Go to original source... Go to PubMed...
    31. Radwan H.S.A., Ammar I.M.A., Eisa A.A., El-Mohymen M.R., Farage A.A. (1984): Some biochemical aspects of certain carbohydrate hydrolyzing enzyme in relation to different insecticidal treatments in the cotton leafworm, Spodoptera littoralis larvae. Bulletin of the Entomological Society of Egypt, Economic Series, 14: 311-319.
    32. Ravi G., Verma S. (1997): Persistence and dissipation of insecticides against Heliothis armigera on chickpea. Indian Journal of Entomology, 59: 62-68.
    33. Salem M.M.I. (1998): Recent approach to minimize pesticide dosages against cotton leafworm using certain adjuvants. [MSc Thesis.] Faculta of Agriculture (Saba Bacha), Alexandria University.
    34. Simpson D.R., Bull D.L, Linquist D.A. (1964): A semimicro technique for the estimation of cholinesterase activity in bullweevil. Annals of the Entomological Society of America, 57:367-377. Go to original source...
    35. Sneddcor G.W., Conchran W.G. (1980): Statistical Methods. 7th Ed. State University Press, Ames.
    36. Sookar A., Farghali A.A., El-Deeb A.Y. (1999): Biochemical effects of the Bacillus thuringiensis (Bactospeine) on the 4th instar larvae cotton leafworm of Spodoptera littoralis (Boisd.). Journal of Agricultural Science Mansoura University, 24: 6937-6943.
    37. Sudhakaran R. (2002): Efficacy of lufenuron (Match 5% EC) against Spodoptera litura (F.) under in vitro condition. Insect Environment, 8: 47-48.
    38. Sun Y.P. (1950): Toxicity index an improved method of comparing the relative toxicity of insecticides. Journal of Economic Entomology, 43: 45-53. Go to original source...
    39. Swelam E.S., Sayed M.A. (2006): Joint action of methomyl, carbaryl, esfenvalerate and profenofos and its latent effect on the cotton leafworm, Spodoptera littoralis. Journal of Pest Control and Environmental Science, 14: 317-331.

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