Plant Protect. Sci., 2012, 48(1):31-36 | DOI: 10.17221/21/2011-PPS

Arbuscular mycorrhizal technology for the growth enhancement of micropropagated Spilanthes acmella Murr

Kuldeep YADAV1, Narender SINGH1, Ashok AGGARWAL2
1 Plant Tissue Culture Laboratory and
2 Mycology and Plant Pathology Laboratory, Department of Botany, Kurukshetra University, Kurukshetra, Haryana, India

Spilanthes acmella (Asteraceae) is an endangered ornamental cum medicinal annual herb. This study reports on the effect of two arbuscular mycorrhizal fungi (Glomus mosseae and Acaulospora laevis) applied either alone or in combination on post-transplanting performance of micropropagated S. acmella. Complete plantlets of S. acmella were raised by direct organogenesis from nodal explants on Murashige and Skoog medium supplemented with various cytokinins. S. acmella plantlets responded to all three mycorrhizal treatments in a significantly different way. G. mosseae enhanced the survival rate of S. acmella plantlets to 100%. Plant height, plant spread, number of branches per plant, number of leaves per plant, leaf area, biomass production, and chlorophyll content were significantly higher in AM inoculated plantlets as compared to the uninoculated ones. This clearly demonstrates that AM inoculation improved not only the survival rate of micropropagated plantlets but also their growth and biomass yield.

Keywords: Glomus mosseae; Acaulospora laevis; Spilanthes acmella; acclimatisation; micropropagation

Published: March 31, 2012  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
YADAV K, SINGH N, AGGARWAL A. Arbuscular mycorrhizal technology for the growth enhancement of micropropagated Spilanthes acmella Murr. Plant Protect. Sci. 2012;48(1):31-36. doi: 10.17221/21/2011-PPS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Aggarwal A., Kumar J., Sharma D., Mehrotra R.S. (1999): Biological control of root rots of sunflower using Trichoderma harzianum and a mycorrhizal fungus. Journal of Indian Botanical Society, 78: 87-97.
    2. Anonymous (1989): The wealth of India: a dictionary of Indian raw materials and industrial products. Vol. 10. Publication and Information Directorate. CSIR, New Delhi: 11-12.
    3. Arnon D.I. (1949): Copper enzymes in isolated chloroplasts polyphenol-oxidase in Beta vulgaris. Plant Physiology, 24: 1-15. Go to original source... Go to PubMed...
    4. Charles P., Raj A.D.S., Kiruba S. (2006): Arbuscular mycorrhizal fungi in the reclamation and restoration of soil fertility. Mycorrhiza News, 18 (2): 13-14.
    5. Clapperton M.J., Reid D.M. (1992): A relationship between plant growth and increasing VA mycorrhizal inoculum density. New Phytologist, 120: 227-234. Go to original source...
    6. Dolcet-Sanjuan R., Claveria E., Camprubi A., Estaun V., Calvet C. (1996): Micropropagation of walnut trees (Juglans regia L.) and response to arbuscular mycorrhizal inoculation. Agronomie, 16: 639-645. Go to original source...
    7. Estrada-Luna A.A., Davies F.T. jr., Egilla J.N. (2000): Mycorrhizal fungi enhancement of growth and gas exchange of micropropagated guava plantlets (Psidium guajava L.) during ex vitro acclimatization and plant establishment. Mycorrhiza, 10: 1-8. Go to original source...
    8. Gaur A., Adholeya A. (1999): Mycorrhizal effects on the acclimatization, survival, growth and chlorophyll of micropropagated Syngonium and Draceana inoculated at weaning and hardening stages. Mycorrhiza, 9:215-219. Go to original source...
    9. Gerdemann J.W., Nicolson Y.H. (1963): Spores of mycorrhiza Endogone species extracted from soil by wet sieving and decanting. Transactions of the British Mycological Society, 46: 235-244. Go to original source...
    10. Gupta A.K., Chaturvedi S., Sharma A.K. (2009): Arbuscular mycorrhizal fungal diversity in some medicinal plants. Mycorrhiza News, 20 (4): 10-13.
    11. Jansen R.K. (1981): Systematics of Spilanthes (CompositaeHeliantheae). Systematic Botany, 6: 231. Go to original source...
    12. Joshee N., Mentreddy S.R., Yadav A.K. (2007): Mycorrhizal fungi and growth and development of micropropagated Scutellaria integrifolia plants. Industrial Crops and Products, 25: 169-177. Go to original source...
    13. Karthikeyan B., Joe M.M., Jaleel C.A. (2009): Response of some medicinal plants to vesicular arbuscular mycorrhizal inoculations. Journal of Scientific Research, 1: 381-386. Go to original source...
    14. Khadir H.A., Zakaria M.B., Ketchil A.A., Azirum M.S. (1989): Toxicity and electro physiological effects of Spilanthes acmella Murr. extracts on Periplaneta americana L. Pesticide Science, 25: 329-335. Go to original source...
    15. Li Q., Ling W., Gao Y., Li F., Xiong W. (2006): Arbuscular mycorrhizal bioremediation and its mechanism of organic pollutants-contaminated soils. Ying Yong Sheng Tai Xue Bao, 17: 2217-2221.
    16. Lovato P.E., Gianinazzi-Pearson V., Trouvelot A., Gianinazzi S. (1996): The state of mycorrhizas and micropropagation. Advances in Horticutural Science, 10: 46-52.
    17. Menge J.A., Timmer L.W. (1982): Procedures for inoculation of plants with vesicular-arbuscular mycorrhizae in the laboratory, greenhouse, and field. In: Schenck N.C. (eds): Methods and Principles of Mycorrhizal Research. American Phytopathological Society, St. Paul: 59-68.
    18. Murashige T., Skoog F. (1692): A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15: 473-497. Go to original source...
    19. Nakatani N., Nagashima M. (1992): Pungent alkamides from Spilanthes acmella L. var. oleracea Clarke. Bioscience, Biotechnology and Biochemistry, 56: 759. Go to original source... Go to PubMed...
    20. Pandey V., Agarwal V. (2009): Efficient micropropagation protocol of Spilanthes acmella L. possessing strong antimalarial activity. In vitro Cellular and Developmental Biology - Plant, 45: 491-499. Go to original source... Go to PubMed...
    21. Panwar J.D.S. (1991): Effect of VAM and Azospirillum brasilense on photosynthesis, nitrogen metabolism and grain yield in wheat. Indian Journal of Plant Physiology, 34: 357-361.
    22. Parkash V., Aggarwal A., Sharma S., Sharma D. (2005): Effect of endophytic mycorrhizae and fungal bioagent on the development and growth of Eucalyptus saligna Sm. seedlings. Bulletin National Institute of Ecology, 15: 127-131.
    23. Rai M.K. (2001): Current advances in mycorrhization in micropropagation. In Vitro Cellular & Developmental Biology - Plant, 37: 158-167. Go to original source...
    24. Rao N.K., Reddy R.K. (1983): Threatened plants of Tirupati and its environs. In: Jain S.K., Rao P.R. (eds): An Assessment of Threatened Plants of India. Deptartment of Environment, Howrah: 167-168.
    25. Reddy B.N., Raghanender C.R., Sreevani A. (2006): Approach for enhancing mycorrhiza mediated disease resistance of tomato damping off. Indian Phytopathology, 59: 299-304.
    26. Saritha K.V., Naidu C.V. (2008): Direct shoot regeneration from leaf explants of Spilanthes acmella. Biologia Plantarum, 52: 334-338. Go to original source...
    27. Sharma D., Kapoor R., Bhatnagar A.K. (2008): Arbuscular mycorrhizal (AM) technology for the conservation of Curculigo orchioides Gaertn.: an endangered medicinal herb. World Journal of Microbiology and Biotechnology, 24: 395-400. Go to original source...
    28. Sheng M., Tang M., Chen H., Yang B., Zhang F., Huang Y. (2009): Influence of arbuscular mycorrhizae on the root system of maize plants under salt stress. Canadian Journal of Microbiology, 55: 879-886. Go to original source... Go to PubMed...
    29. Singh V. (1995): Herbal folk remedies of Morni hills (Haryana), India. Fitoterapia, 66: 425-430.
    30. Singh S.K., Rai M.K., Asthana P., Sahoo L. (2009): An improved micropropagation of Spilanthes acmella L. through transverse thin cell layer culture. Acta Physiologiae Plantarum, 31: 693-698. Go to original source...
    31. Wang H., Parent S., Gosselin A. (1993): Study of vesicular arbuscular mycorrhizal peat-based substrates on symbiosis establishment, acclimatization and growth of three micropropagated species. Journal of American Society for Horticultural Sciences, 118: 896-901. Go to original source...
    32. Wilson J., Munro R.C., Inglely K., Mason P.A., Jefwa M.P.N., Dick J.M.C.P., Leaky R.R.B. (1991): Tree establishment in semiarid lands of Kenya - Role of mycorrhizal inoculation and water-retaining polymer. Forest Ecology and Management, 45: 153-163. Go to original source...
    33. Yadav K., Singh N., Aggarwal A. (2011): Influence of arbuscular mycorrhizal (AM) fungi on survival and development of micropropagated Acorus calamus L. during acclimatization. Journal of Agricultural Technology, 7: 775-781.
    34. Yadav K., Singh N. (2010): Micropropagation of Spilanthes acmella Murr. - An important medicinal plant. Nature and Science, 8 (9): 5-11.

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content