Plant Protect. Sci., 2012, 48(2):80-84 | DOI: 10.17221/49/2011-PPS

Fusarium verticillioides from sugarcane, vegetative compatibility groups and pathogenicity

Abbas Mohammadi1, Reza Farrokhi Nejad2, Nasrin Noras Mofrad3
1 Department of Plant Pathology, Faculty of Agriculture, Birjand University, Birjand, Iran
2 Department of Plant Pathology, Faculty of Agriculture, Shahid Chamran University, Ahwaz, Iran
3 Faculty of Agriculture, Islamic Azad University Birjand Branch, Birjand, Iran$2

Sugarcane plant samples affected by the disease pokkah boeng were collected from the major sugarcane producing areas in Iran. Fifty isolates of Fusarium verticillioides were recovered from diseased sugarcanes. Results of pathogenicity tests revealed that all isolates were pathogenic to sugarcane. Four hundred and eighty-five nitrate non-utilising mutants were generated from F. verticillioides isolates. Media used for nit mutant generation included potato dextrose agar, minimal medium and Czapeck agar amended with 1.8%, 2%, 2.5%, and 3% potassium chlorate. Nit mutants were divided into three phenotypic (nit1, nit3, and nitM) classes based on their growth on different nitrogen sources in the culture media. Among the isolates tested, 280, 115 and 90 nit1, nit3, and nit M were generated, respectively. Nit mutants were used to force the heterokaryon formation to determine the distribution of VCGs and their relation to pathogenicity and geographic origin. Forty-eight VCGs of F. verticillioides were isolated from sugarcane. Forty-six of the VCGs were represented by a single isolate, whereas the remaining two comprised two isolates. None of the VCGs was common.

Keywords: pokkah boeng; Fusarium; mutant; nit

Published: June 30, 2012  Show citation

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Mohammadi A, Nejad RF, Mofrad NN. Fusarium verticillioides from sugarcane, vegetative compatibility groups and pathogenicity. Plant Protect. Sci. 2012;48(2):80-84. doi: 10.17221/49/2011-PPS.
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