Plant Protect. Sci., 2013, 49(4):165-168 | DOI: 10.17221/82/2012-PPS

Leaf spot and dieback of Buxus caused by Cylindrocladium buxicola Original Paper

Ivana ŠAFRÁNKOVÁ, Ludmila HOLKOVÁ, Martin KMOCH
Department of Crop Science, Breeding and Plant Medicine, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic

Leaf blight symptoms were observed on potted box plants (Buxus sempervirens cv. Suffruticosa and B. microphylla) in a nursery in South Moravia in August 2010. These symptoms were suggestive of box blight on Buxus spp. Characteristics of the visual symptoms, microscopic features, and identification of the isolates using DNA sequencing are described. The causal agent was isolated and identified as Cylindrocladium buxicola Henricot by means of morphological, cultural, and molecular characters. The effect of five commercial fungicides on C. buxicola in vitro was studied. The most effective fungicides (kresoxim-methyl, azoxystrobin, and mancozeb) inhibited conidia germination and mycelial growth (kresoxim-methyl, myclobutanil, and penconazole) of C. buxicola more than 96%.

Keywords: box blight; box; fungicides

Published: December 31, 2013  Show citation

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ŠAFRÁNKOVÁ I, HOLKOVÁ L, KMOCH M. Leaf spot and dieback of Buxus caused by Cylindrocladium buxicola . Plant Protect. Sci. 2013;49(4):165-168. doi: 10.17221/82/2012-PPS.
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    References

    1. Bolland L., Tierney J.W., Tierney B.J. (1985): Studies on leaf spot and shoot blight of Eucalyptus caused by Cylindrocladium quinqueseptatum. European Journal of Forest Pathology, 15: 385-397. Go to original source...
    2. Brand T. (2005): Auftreten von Cylindrocladium buxicola B. Henricot an Buchsbaum in Nordwest Deutschland. Nachrichtenblatt des Deutschen Pflanzenschutzdienst, 57: 237-240.
    3. Brand T. (2006): In-vitro-Wirkung fungizider Wirkstoffe auf Konidienkeimung und Myzelwachstum von Cylindrocladium buxicola. Nachrichtenblatt des Deutschen Pflanzenschutzdienst, 58: 117-121.
    4. Cech T., Diminic T.D., Heungens K. (2010): Cylindrocladium buxicola caused common box blight in Croatia. Plant Pathology, 59: 1169. Go to original source...
    5. Crepel C., Inghelbrecht S. (2003): First report of blight on Buxus spp. caused by Cylindrocladium buxicola in Belgium. Plant Disease, 87: 1539. Go to original source... Go to PubMed...
    6. Glass N.L., Donaldson G.C. (1995): Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Applied and Environmental Microbiology, 61: 1323-1330. Go to original source... Go to PubMed...
    7. Haralson J.C., Brannen P.M., Scherm H., NeSmith D.S. (2009): Evaluation of fungicides for the control of Cylindrocladium root rot in blueberry cuttings, 2008. Plant Disease Management Reports, 3: SMF039.
    8. Henricot B. (2003): Box blight. Topiarius, 6: 28-30.
    9. Henricot B. (2006): Box blight rampages onwards. Plantsman, 13: 153-157. Go to original source...
    10. Henricot B., Culham C. (2002): Cylindrocladium buxicola, a new species affecting Buxus spp., and its phylogenetic status. Mycologia, 94: 980-997. Go to original source... Go to PubMed...
    11. Henricot B., Peréz Siera A., Prior C. (2000): A new blight disease on Buxus in the UK caused by the fungus Cylindrocladium. Plant Pathology, 49: 805. Go to original source...
    12. Henricot B., Gorton C., Denton G., Denton J. (2008): Studies on the control of the Cylindrocladium buxicola using fungicides and host resistance. Plant Diseases, 92: 1273-1279. Go to original source... Go to PubMed...
    13. Jayasinghe C.K., Wijesundera R.L.C. (1995): In vitro evaluation of fungicides against clove isolate of Cylindrocladium quinqueseptatum in Sri Lanka. International Journal of Pest Management, 4: 219-223. Go to original source...
    14. O'Donnell K., Cigelnik E. (1997): Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous. Molecular Phylogenetics and Evolution, 7: 103-116. Go to original source... Go to PubMed...
    15. Šafránková I., Kmoch M., Holková L. (2012): First report of Cylindrocladium buxicola on box in the Czech Republic. New Disease Reports, 25: 5. Go to original source...
    16. Varela C.P., Penalta J.P., Vázquez P.M., Casal O.A. (2009): First report of Cylindrocladium buxicola on Buxus sempervirens in Spain. Plant Diseases, 93: 670. Recieved for publication November 5, 2012 Go to original source... Go to PubMed...

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