Plant Protect. Sci., 2014, 50(2):84-89 | DOI: 10.17221/71/2012-PPS

Biochemical characterisation of α-amylase in two aphid species, Aphis fabae Scopoli (Hemiptera: Aphididae) and A. gossypii Glover (Hemiptera: Aphididae)Original Paper

Ali Darvishzadeh, Ali Reza Bandani, Seyed Qasem Mousavi
Plant Protection Department, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

We identify and characterise α-amylases of the two aphid species, A. fabae and A. gossypi. To do this, α-amylases of the two insect species were extracted and their activities were determined using 1% soluble starch. Results showed that α-amylase, which hydrolyses starch, is present in both aphids. Also, it was shown that optimum pH and temperature for the α-amylases of both species is 7.0 and 40°C, respectively. Gel assays using zymogram analysis showed that in both aphid species more than one isoform (two isoforms) of α-amylases hydrolyszing carbohydrates are present.

Keywords: aphids; gel assay; starch

Published: June 30, 2014  Show citation

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Darvishzadeh A, Bandani AR, Mousavi SQ. Biochemical characterisation of α-amylase in two aphid species, Aphis fabae Scopoli (Hemiptera: Aphididae) and A. gossypii Glover (Hemiptera: Aphididae). Plant Protect. Sci. 2014;50(2):84-89. doi: 10.17221/71/2012-PPS.
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