Plant Protect. Sci., 2015, 51(3):115-123 | DOI: 10.17221/3/2015-PPS

Emergence of quarantine Tobacco ringspot virus in Impatiens walleriana in the Czech RepublicOriginal Paper

Jiban Kumar KUNDU1, Sebastien GADIOU1, Gabriela SCHLESINGEROVÁ2, Miroslava DZIAKOVÁ2, Václav ČERMÁK2
1 Division of Crop Protection and Plant Health, Crop Research Institute, Prague, Czech Republic
2 Division of Diagnostics, Central Institute for Supervising and Testing in Agriculture, Olomouc, Czech Republic

Tobacco ringspot virus (TRSV) is a quarantine pathogen in Europe. During an official inspection in November 2011, Impatiens walleriana plants showing symptoms were found in a nursery in the Czech Republic. The causal agent of the disease was detected as Nepovirus group A by RT-PCR using specific primers of the Nepovirus group. Sequence analysis of PCR fragments confirms that the detected virus is TRSV. TRSV detection in these plants was further confirmed by ELISA and one-step RT-PCR using specific primers. The coat protein (CP) gene of the Czech TRSV isolate was sequenced, and the sequence analysis showed high identity of both nucleotide (99.28%) and amino acid (99.96%) levels compared with other known TRSV isolates from GenBank. Two amino acid motifs characteristic of nepoviruses, FDDY (FDAY) and FWGR (FYGR), were found equally at positions 80 and 497 of the TRSV CP genes, respectively, including the sequences described in this study.

Keywords: TRSV; RT-PCR; quarantine pathogen

Published: September 30, 2015  Show citation

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KUNDU JK, GADIOU S, SCHLESINGEROVÁ G, DZIAKOVÁ M, ČERMÁK V. Emergence of quarantine Tobacco ringspot virus in Impatiens walleriana in the Czech Republic. Plant Protect. Sci. 2015;51(3):115-123. doi: 10.17221/3/2015-PPS.
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