Plant Protect. Sci., 2015, 51(3):127-135 | DOI: 10.17221/86/2014-PPS

Exogenous application of spermidine enhanced tolerance of pepper against Phytophthora capsici stressOriginal Paper

Esra KOÇ
Department of Biology, Faculty of Science, Ankara University, Ankara, Turkey

The effect of exogenous spermidine - Spd (0.1 and 1 mM) on the relation between polyaminoxidase (PAO), diaminoxidase (DAO), H2O2, and malondialdehyde (MDA) in three cultivars of pepper (Capsicum annum L.) exhibiting different tolerance to P. capsici stress: KM-Hot (P. capsici-tolerant), PM-217 (P. capsici-resistant), and CM-334 (P. capsici-highly resistant) was investigated. The 0.1 mM Spd pre-treatment led to an increase in DAO activity on the third day in three pepper cultivars under the stress of P. capsici, 1 mM Spd + P. capsici led to an increase in DAO and PAO activities on the fifth day if compared to P. capsici treatment alone. P. capsici alone caused an increase in the amounts of H2O2 at all times in all cultivars and in the amounts of MDA on the third and fifth days in all cultivars. Conversely; under the stress of P. capsici, pre-application of 0.1 mM Spd at all times in KM-Hot and CM-334 cultivars decreased the amount of MDA and H2O2 and on the first and third days in PM-217 cultivar decreased the amount of MDA and H2O2. This data indicates that exogenous Spd application before inoculation decreases the plasma membrane injury by decreasing the level of H2O2 and regulating the activities of amine oxidasein both P. capsici-sensitiveand P. capsici-resistant cultivars of peppers, so it may increase the tolerance of pepper cultivars against P. capsici.

Keywords: amine oxidase; oxidative stress; pepper; pathogen; polyamine

Published: September 30, 2015  Show citation

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KOÇ E. Exogenous application of spermidine enhanced tolerance of pepper against Phytophthora capsici stress. Plant Protect. Sci. 2015;51(3):127-135. doi: 10.17221/86/2014-PPS.
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    References

    1. Alcazar R., Cuevas J.C., Planas J., Zarza X., Bortolatti C., Carrasco Salinas P., Tiburcio J., Altabella A.F. (2011): Integration of polyamines in the cold acclimation response. Plant Science, 180: 31-38. Go to original source... Go to PubMed...
    2. Angelini R., Cona A., Federico R., Fincato P., Tavladoraki P., Tisi A. (2010): Plant amine oxidases "on the move": an update. Plant Physiology and Biochemistry, 48: 560-564. Go to original source... Go to PubMed...
    3. Bestwik C.S., Brown I.R.,, Bennett M.H.R., Mansfield J.W. (1997): Localisation hydrogen peroxide accumulation during the hypersensitive reaction of lettuce calls to Pseudomonas syringae pv. phaseolicola. Plant Cell, 9: 209-221. Go to original source... Go to PubMed...
    4. Cona A., Cenci F., Cervelli M., Federico R., Mariottini P. (2003): Polyamine oxidase, a hydrogen peroxide-producing enzyme, is upregulated by light and down-regulated by auxin in the outer tissues of the maize mesocotyl. Plant Physiology, 131: 803-813. Go to original source... Go to PubMed...
    5. Cona A., Rea G., Angelini R., Federico R., Tavladoraki P. (2006): Functions of amine oxidases in plant development and defence. Trends in Plant Science, 11: 80-88. Go to original source... Go to PubMed...
    6. Cowley T., Walters D.R. (2002): Polyamine metabolism in barley reacting hypersensitively to the powdery mildew fungus Blumeria graminis f.sp. hordei. Plant Cell Environment, 25: 461-468. Go to original source...
    7. Gupta K., Dey A., Gupta B. (2013): Plant polyamines in abiotic stress responses. Acta Physiologia Plantarum, 34: 2015-2035. Go to original source...
    8. Harrigan W.F., McCane M.E. (1966): Laboratory Methods in Microbiology, Recipes to Stains, Reagents and Media. London and New York, Academic Press.
    9. Heath R.L., Packer L. (1968): Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics, 125: 180-98. Go to original source... Go to PubMed...
    10. Holmstedt B., Larsson L., Tham R. (1961): Further studies of spectrophotometric method for determination of diamine oxidase activity. Biochimica et Biophysica Acta, 48: 182-186. Go to original source... Go to PubMed...
    11. Hu X., Zhang Y., Shi Y., Zhang Z., Zou Z., Zhang H., Zhao J. (2012): Effect of exogenous spermidine on polyamine content and metabolism in tomato exposed to salinity-alkalinity mixed stress. Plant Physiology and Biochemistry, 57: 200-209. Go to original source... Go to PubMed...
    12. Hussain S.S., Ali M., Ahmad M., Siddique K.H. (2011): Polyamines: natural and engineered abiotic and biotic stress tolerance in plants. Biotechnology Advances, 29: 300-311. Go to original source... Go to PubMed...
    13. Jones D.R., Unwin C.H., Ward E.W.B. (1975): Capsidiol induction in pepper fruit during interactions with Phytophthora capsici and Monilinia fructicola. Phytopathology, 65: 1417-1419. Go to original source...
    14. Kim Y.J., Hwang B.K., Park K.W. (1989): Expression of age-related resistance in pepper plants infected with P. capsici. Plant Disease, 73: 745-747. Go to original source...
    15. Koç E., Ustun A.S., Iºlek C., Arici Y.K. (2011): Defence responses in leaves of resistant and susceptible pepper (Capsicum annuum L.) cultivars infected with different inoculum concentrations of Phytophthora capsici Leon. Scientia Horticulturae, 128: 434-442. Go to original source...
    16. Kongkiattikajorn J. (2009): Effect of salinity stress on degradation of polyamines and amine oxidase activity in maize seedlings. Kasetsart Journal (Natural Science), 43: 28-33.
    17. Kubis J. (2005): The effect of exogenous spermidine on superoxide dismutase activity, H2O2 and superoxide radical level in barley leaves under water deficit conditions. Acta Physiologia Plantarum, 27: 289-295. Go to original source...
    18. Moschou P.N., Sarris P.F., Skandalis N., Andriopoulou A.H., Paschalidis K.A., Panopoulos N.J. (2009): Engineered polyamine catabolism preinduces tolerance of tobacco to bacteria and oomycetes. Plant Physiology, 149: 1970-1981. Go to original source... Go to PubMed...
    19. Palloix A., Daubezeand A.M., Pochard E. (1988): Phytophthora root rot of pepper. Influence of host genotype and pathogen strain on the inoculum density-disease severity relationships. Journal of Phytopathology, 123: 25-33. Go to original source...
    20. Paschalidis K.A., Roubelakis-Angelakis K.A. (2005): Sites and regulation of polyamine catabolism in the tobacco plant. Correlations with cell division-expansion, cell cycle progression and vascular development. Plant Physiology, 138: 2174-2184. Go to original source... Go to PubMed...
    21. Rea G., Pinto M.C.D., Tavassa R., Biondi S., Gobbi V., Ferrante P., Gara L.D., Federico R., Angellini R., Tavladoraki P. (2004): Ectopic expression of maize polyamine oxidase and pea copper amine oxidase in the cell wall of tobacco plants. Plant Physiology, 134: 1414-1426. Go to original source... Go to PubMed...
    22. Rodriguez K.M., Ruiz O.A., Maiale S., Ruiz-Herrera j., Jimenez B.J.F. (2008): Polyamine metabolism in maize tumors induced by Ustilago maydis. Plant Physiology and Biochemistry, 46: 805-814. Go to original source... Go to PubMed...
    23. Roychoudhury A., Ban S., Sengupta D.N. (2011): Amelioration of salinity stress by exogenously applied spermidine and spermine in three varieties of indice rice differing in their level of salt tolerance. Journal of Plant Physiology, 168: 317-328. Go to original source... Go to PubMed...
    24. Rybkwoska M.S., Borucki W. (2014): Localization of hydrogen peroxide accumulation and diamine oxidase activity in pea root nodules under aluminum stress. Micron, 57: 13-22. Go to original source... Go to PubMed...
    25. Velikova V., Yordanov I., Edreva A. (2000): Oxidative stress and some antioxidant system in acid rain treated bean plants: protective role of exogenous polyamines. Plant Science, 151: 59-66. Go to original source...
    26. Walters D.R. (2003): Resistance to plant pathogens: possible roles for free polyamines and polyamine catabolism. New Phytologist, 59: 109-115. Go to original source... Go to PubMed...
    27. Wan X., Shi G.X., Xu Q.S. (2007): Exogenous polyamines enhance copper tolerance of Nymphoides peltatum. Journal of Plant Physiology, 164: 1062-1070. Go to original source... Go to PubMed...
    28. Ward E.W.B., Stoessl A. (1974): Isolation of the phyoalexin capsidiol from pepper leaves and stems. In: 66 th Annual Meeting American Phytopathological Society, Aug 11-15, 1974, Vancouver, Canada: 11-15.
    29. Xu X., Guoxin S., Chunxiai D., Ye X., Juan Z., Haiyan Y. (2011): Regulation of exogenous spermidine on the reactive oxygen species level and polyamine metabolism in Alternanthera philoxeroides (Mart.) Griseb under copper stress. Plant Growth Regulation, 63: 251-258. Go to original source...
    30. Yoda H., Yamaguchi Y., Sano H. (2003): Induction of hypersensitive cell death by hydrogen peroxide through polyamine degradation in tobacco plants. Plant Physiology, 132: 1973-1981. Go to original source... Go to PubMed...
    31. Yoda H., Hiroi Y., Sano H. (2006): Polyamine oxidase is one of the key elements for oxidative burst to induce programmed cell death in tobacco cultured cells. Plant Physiology, 142: 193-206. Go to original source... Go to PubMed...

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