Plant Protect. Sci., 2015, 51(4):177-190 | DOI: 10.17221/104/2014-PPS

Real-time PCR applied to study on plant pathogens: potential applications in diagnosis - a reviewReview

Seyed Mahyar MIRMAJLESSI1, Evelin LOIT1, Marika MÄND2, Seyed Mojtaba MANSOURIPOUR3
1 Department of Field Crops and Grassland Husbandry and
2 Department of Plant Protection, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Tartu, Estonia
3 Department of Plant Pathology, North Dakota State University, Fargo, USA$2

Quantitative real-time PCR (qPCR) technique incorporates traditional polymerase chain reaction (PCR) efficiency with the production of a specific fluorescent signal, measuring the kinetics of the reaction in the early PCR phases and providing quantification of specific targets in various environmental samples. There are an increasing number of chemistries to detect PCR products, which are widely used in plant pathology as they cluster into the amplicon sequence non-specific and sequence-specific techniques. In this review, we illustrate a general description of major chemistries and discuss some considerations for assay development as it applies for a wide range of applications in epidemiological studies. The technique has become the gold standard for early detection of pathogens and a fundamental tool in the research laboratory.

Keywords: bacteria; fungi; oomycetes; phytoplasmas; viroids; viruses; plants; quantification; polymerase chain reaction; qPCR chemistries

Published: December 31, 2015  Show citation

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MIRMAJLESSI SM, LOIT E, MÄND M, MANSOURIPOUR SM. Real-time PCR applied to study on plant pathogens: potential applications in diagnosis - a review. Plant Protect. Sci. 2015;51(4):177-190. doi: 10.17221/104/2014-PPS.
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