Plant Protect. Sci., 2016, 52(1):1-9 | DOI: 10.17221/63/2015-PPS

Recent approaches for controlling downy mildew of cucumber under greenhouse conditionsOriginal Paper

Ahmed Mohamed2, Amany Hamza1, Aly Derbalah1
1 Pesticides Chemistry and Toxicology Department, Faculty of Agriculture, Kafr-El-Shiekh University, Kafr El-Sheikh, Egypt
2 Plant Pathology Research Institute, Agricultural Research Centre, Giza, Egypt

The efficacy of biocontrol agents (effective microorganisms (EMs1), Bacillus subtilus, and Bacillus pumilus), zinc oxide nanoparticles, castor and clove oils, as well as of a recommended fungicide (famoxadone + cymoxanil) utilised during two growing seasons under greenhouse conditions against downy mildew of cucumber were evaluated. Furthermore, GC-MS analysis was carried out to identify the bioactive chemical components of plant origin oils (castor and clove). The effect of these treatments on some biochemical, growth, and yield characters of cucumber was also investigated. Famoxadone + cymoxanil was the most effective treatment in both test seasons, followed by clove oil, zinc oxide, EMs1, Bacillus pumilus, Bacillus subtilus, and castor oil. The results showed a significant effect of all the treatments on some biochemical (chlorophyll content, peroxidase, and polyphenoloxidase) as well as growth and yield characters (plant height, fruit number per plant, and fruit yield) of the cucumber plants relative to control.

Keywords: plant pathogen; cucurbits; plant oils; nanoparticles; bioagents

Published: March 31, 2016  Show citation

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Mohamed A, Hamza A, Derbalah A. Recent approaches for controlling downy mildew of cucumber under greenhouse conditions. Plant Protect. Sci. 2016;52(1):1-9. doi: 10.17221/63/2015-PPS.
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    References

    1. Agoramoorthy G., Chandrasekaran M., Venkatesalu V., Hsu J. (2007): Antibacterial and antifungal activities of fatty acid methyl esters of the blind-your-eye mangrove from India. Brazil Journal of Microbiology, 38: 739-742. Go to original source...
    2. Allam A.I., Hollis S.P. (1972): Sulfide inhibition of oxidase in rice root. Phytopathology, 62: 634-639. Go to original source...
    3. Anonymous A. (2005): Bacillus subtilis strain QST 713. Biopesticide Registration Action Document 006479.
    4. Ayoola G.A., Coker H.A.B., Adesegun S.A., Adepoju-Bello A.A., Obaweya K., Ezennia E.C., Atangbayil, T.O. (2008): Phytochemical screening and antioxidant activities of some selected medicinal plants used for malaria therapy in South Western Nigeria. Tropical Journal of Pharmacology Research, 7: 1019-1024. Go to original source...
    5. Bakkali, F., Averbeck S., Averbeck D., Idaomar M. (2008): Biological effects of essential oils e a review. Food and Chemical Toxicology, 46: 446-475. Go to original source... Go to PubMed...
    6. Bernhaedt E., Dodson J., Waterson J. (1988): Cucurbit Diseases: a Practical Guide for Seeds Men, Growers and Agricultural Advisors. Saticoy, Petoseed Co.
    7. Bharathi R., Vivekananthan R., Harish S., Ramanathan A., Samiyappan R. (2004): Rhizobacteria-based bio-formulations for the management of fruit rot infection in chilies. Crop Protection, 23: 835-843. Go to original source...
    8. Bowers J.H., Locke J.C. (2000): Effect of botanical extracts on the population density of Fusarium oxysporum in soil and control of Fusarium wilt in the greenhouse. Plant Disease, 84: 300-305. Go to original source... Go to PubMed...
    9. Bowers J.H., Locke J.C. (2004): Effect of formulated plant extracts and oils in population density of Phytophthora nicotianae in soil and control of Phytophthora blight in the greenhouse. Plant Disease, 88: 11-16. Go to original source... Go to PubMed...
    10. Brannen P.M., Kenney D.S. (1997): Successful biologicalcontrol product for suppression of soil-borne plant pathogens of cotton. Journal of Indian Microbiology and Biotechnology, 19: 169-171. Go to original source...
    11. Cardwell K.F., Schultless F., Ndemah R., Nogok Z. (1997): A systems approach to assess crop health and maize yield losses due to pests and diseases in Cameron. Agriculture, Ecosystems and Environment, 65: 33-47. Go to original source...
    12. Chen C., Belanger R.R., Benhamou N., Paulitz T. (2000): Defense enzymes induced in cucumber roots by treatment with plant growth promoting rhizobacteria (PGPR) and Pythium aphanidermatum. Physiolological and Molecular Plant Pathology, 56: 13-23. Go to original source...
    13. Collins D.P., Jacobsen B. (2003): Optimizing a Bacillus subtilis iso­late for biological control of sugar beet Cercospora leaf spot. Biological Control Journal, 26: 153-161. Go to original source...
    14. Commare R.R., Nandakumara R., Kandana A., Suresh S., Bharathi M., Raguchander T., Samiyappan R. (2002): Pseudomonas fluorescense based bio-formulation for the management of sheath blight disease and leaf-folder insect in rice. Crop Protection, 21: 671-677. Go to original source...
    15. Cook R.J. (1988): Biological control and holistic planthealth care in agriculture. American Journal of Alternative Agriculture, 3: 51-62. Go to original source...
    16. Derbalah A.S., El-Moghazy S.M., Godah M.I. (2013): Alternatives methods to control of sugar beet leaf spot disease caused by the fungus Cercospora beticola (Sacc). Egyptian Journal of Biological Pest Control, 23: 2 247-254.
    17. Descalzo R.C., Rohe J.E., Mauza B. (1990): Comparative efficacy of induced resistance for selected diseases of greenhouse cucumber. Canadian Journal of Plant Pathology, 12: 69-79. Go to original source...
    18. Duncan D.B. (1955): Multiple range and multiple F test. Biometrics, 11: 1-42. Go to original source...
    19. Dunlop R.W., Simon A., Sivasithamparam K. (1989): An antibiotic from Trichoderma koningii active against soil borne plant pathogens. Journal of Natural Products, 52: 67-74. Go to original source...
    20. Elad Y., Kapa A. (1999): Role of Trichoderma harzianum protease in the biocontrol of Botrytis cinerea. European Journal of Plant Pathology, 105: 177-189. Go to original source...
    21. Falk S.P., Gadoury D.M., Cortesi P., Pearson R.C., Seem R.C. (1995): Parasitism of Uncinula necator ascomata by the mycoparasite Ampelomyces quisqualis. Phytopathology, 85: 794-800. Go to original source...
    22. Fang M., Chen J.H., Xu X.L., Yang P.H., Hildebrand H.F. (2006): Antibacterial activities of inorganic agents on six bacteria associated with oral infections by two susceptibility tests. International Journal of Antimicrobial Agents, 27: 513-517. Go to original source... Go to PubMed...
    23. Handelsman J., Stabb E.V. (1996): Biocontrol of soil borne plant pathogens. Plant Cell, 8: 1855-1869. Go to original source...
    24. Hansen M.A. (2000): Downy Mildew of Cucurbits. Publication Number 450-707. Virginia Cooperative Extension. Available at http://www.ext.vt.edu/pubs/plantdiseasefs/450-707/450-707.html
    25. Harman G.E. (2000): Myths and dogmas of biocontrol: changes imperceptions derived from research on Trichoderma harzianumT-22. Plant Disease, 84: 377-393. Go to original source... Go to PubMed...
    26. Howell C.R., Stipanovic R.D., Lumsden R.D. (1993): Antibiotic production by strains of Gliocladium virens and its relation to the biocontrol of cotton seedling diseases. Biocontrol Science and Technology, 3: 435-440. Go to original source...
    27. Heydari A., Misaghi I.J. (2003): The role of rhizosphere bacteria in herbicide-mediated increase in Rhizoctonia solani-induced cotton seedling damping-off. Plant and Soil, 257: 391-396. Go to original source...
    28. Jin T., Sun D., Su J.Y., Zhang H., Sue H. (2009): Antimicrobial efficacy of zinc oxide quantum dots against L. monocytogenes, S. enteritidis, and E. coli O157:H7. Journal of Food Science, 74: M46-M52. Go to original source... Go to PubMed...
    29. Kendrick B. (1992): The Fifth Kingdom Mycologue Publications. Waterloo, Mycologue Publications.
    30. Kim K.J., Sung W.S., Moon S.K., Choi J.S., Kim J.G., Lee D.G. (2008a): Antifungal effect of silver nanoparticles on dermato-phytes. Journal of Microbiology and Biotechnology, 18: 1482-1484. Go to PubMed...
    31. Kumar A., Vemula P.K., Ajayan P.M., John G. (2008): Silvernanoparticle-embedded antimicrobial paints based on vegetable oil. Nature Materials, 7: 236-241 Go to original source... Go to PubMed...
    32. Khoshgoftarmanesh A.H., Schulin R., Chaney R.L., Daneshbakhsh B., Afyuni M. (2010): Micronutrient-efficient genotypes for crop yield and nutritional quality in sustainable agriculture. A review. Agronomy for Sustainable Development, 30: 83-107. Go to original source...
    33. Matta A., Diamond A.E. (1963): Symptoms of Fusarium wilt in relation to quantity of Fusarium and enzyme activity in tomato stems. Plant Pathology, 53: 574-578.
    34. Momol M.T., Mitchell D.J., Rayside P.A., Olson S.M., Momol E.A. (2000): Plant essential oils as potential biofumigants for the management of soil borne pathogens of tomato. Phytopathology, 90: S127.
    35. Moran R., Porath D. (1980): Chlorophyll determination in intact tissues using N,N-dimethylformamide. Plant Physiology, 65: 478-479. Go to original source... Go to PubMed...
    36. Nandakumar R., Babu S., Viswanathan R., Raghuchander T., Samiyappan R. (2001): Induction of systemic resistance in rice against sheath blight disease by Pseudomonas fluorescens. Soil Biology & Biochemistry, 33: 603-612. Go to original source...
    37. Nieman C. (1995): Influence of trace amounts of fatty acids on the growth of microorganisms. Bacteriology Reviews, 18: 147-163. Go to original source... Go to PubMed...
    38. Obagwu J., Korsten L. (2003): Integrated control of citrus green and blue molds using Bacillus subtilis in combination with sodium bicarbonate or hot water. Postharvest Biology and Technology, 28: 187-194. Go to original source...
    39. Ohira T., Yamamoto O., Iida Y., Nakagawa Z. (2008): Antibacterial activity of ZnO powder with crystallographic orientation. Journal of Materials Science: Materials in Medicine, 19: 1407-1412. doi: 10.17221/63/2015-PPS Go to original source... Go to PubMed...
    40. Oka Y., Nacar S., Putievsky E., Ravid U., Yaniv Z., Spiegel Y. (2000): Nematicidal activity of essential oils and their components against the root-knot nematode. Phytopathology, 90: 710-715. Go to original source... Go to PubMed...
    41. Romero D., De Vicente A., Zeriouh H., Cazorla F.M., Fernández-Ortuño D., Torés J.A., Pérez-García A. (2008): Evaluation of biological control agents for managing cucurbit powdery mildew on greenhouse-grown melon. Plant Pathology, 56: 976-986. Go to original source...
    42. Russell J.B., Diaz-Gonzalez F. (1998): The effects of fermentation acids on bacterial growth. Advances in Microbial Physiology, 39: 205-234. Go to original source... Go to PubMed...
    43. Sagdic O., Karahan A.G., Ozcan M., Ozkan G. (2003): Effect of some spice extracts on bacterial inhibition. Food Science and Technology International, 9: 353-356. Go to original source...
    44. Salgueiro L., Martins A.P., Correia H. (2010): Raw materials: the importance of quality and safety. A review. Flavour and Fragrance Journal, 25: 253-271. Go to original source...
    45. Shahraki M., Heydari A., Hassanzadeh N., Rezaei S., Naraghi L. (2008): Investigation on the possibility of biological control of sugar beet seedling damping-off disease. Iranian Journal of Agricultural Science, 13: 23-38.
    46. Shahraki M., Heydari A., Hassanzadeh N. (2009): Investigation of antibiotic, siderophore and volatile metabolite production by bacterial antagonists against Rhizoctonia solani. Iranian Journal of Biology, 22: 71-84.
    47. Shewry P.R., Lucas J.A. (1997): Plant proteins that confer resistance to pests and pathogens. Advances in Botanical Research, 26: 135-192. Go to original source...
    48. Sivakumar G., Sharma R.C. (2003): Induced biochemical changes due to seed bacterization by Pseudomonas fluorescens in maize plants. Indian Phytopathology, 56: 134-137.
    49. Skřivanová E., Marounek M., Dlouhá G., Kaňka J. (2005): Susceptibility of Clostridium perfringens to C2-C18 fatty acids. Letter in Applied Microbiology, 41: 77-81. Go to original source... Go to PubMed...
    50. Sprong R.C., Hulstein M.F.E., van der Meer R. (2001): Bactericidal activities of milk lipids. Antimicrobial Agents and Chemotherapy, 45: 1298-1301. Go to original source... Go to PubMed...
    51. Stoimenov P.K., Klinger R.L., Marchin G.L., Klabunde J.S. (2002): Metal oxide nanoparticles as bactericidal agents. Langmuir, 18: 6679-6686. Go to original source...
    52. Sunanda K., Kikuchi Y., Hashimoto K., Fujishima A. (1998): Bactericidal and detoxification effects of TiO2 thin film photocatalysts. Environmental Science and Technology, 32: 726-728. Go to original source...
    53. Szczech M., Shoda M. (2004): Biocontrol of Rhizoctonia damping-off of tomato by Bacillus subtilis combined with Burkholderia cepacia. Journal of Phytopathology, 152: 549-556. Go to original source...
    54. Xiao-Fang G., Ze-Bin W., Qi-Tang W., Jin-Rong Q., JianLi Z. (2011): Cadmium and zinc accumulation in maize grain as affected by cultivars and chemical fixation amendments. Pedosphere, 21: 650-656. Go to original source...
    55. Zdor R.E., Anderson A.J. (1992): Influence of root colonizing bacteria on the defense responses of bean. Plant and Soil, 140: 99-107. Go to original source...

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