Plant Protect. Sci., 2016, 52(3):167-175 | DOI: 10.17221/70/2015-PPS

Isolation and identification of antifungal compounds produced by Bacillus Y-IVI for suppressing Fusarium wilt of muskmelonOriginal Paper

Qingyun Zhao1, 2, Xinlan Mei1, Yangchun Xu1
1 Jiangsu Key Lab for Organic Waste Utilization and National Engineering Research Center for Organic-based Fertilizers, Nanjing Agricultural University, Nanjing, P.R. China
2 Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, P.R. China

The Bacillus strain Y-IVI was used in the biocontrol of muskmelon Fusarium wilt. It was identified as Bacillus subtilis. The antifungal compounds from the culture filtrate were purified by high performance liquid chromatography. Two series of homologous ion peaks were analysed by liquid chromatography-electrospray ionisation-mass spectrometry, one with molecular weights of 1028.7, 1042.7, and 1056.7 and the other with molecular weights of 1463, 1477, and 1491. The compounds were ascribed to iturin A and fengycin, respectively. The maximum production of iturin by Y-IVI was 89.75 mg/l. In conclusion, we provided biochemical evidence that strain Y-IVI was able to produce antifungal compounds and thus holds great potential for use in the biocontrol of Fusarium wilt disease.

Keywords: antifungal substance; Bacillus subtilis; biological control; Cucumis melo L.

Published: September 30, 2016  Show citation

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Zhao Q, Mei X, Xu Y. Isolation and identification of antifungal compounds produced by Bacillus Y-IVI for suppressing Fusarium wilt of muskmelon. Plant Protect. Sci. 2016;52(3):167-175. doi: 10.17221/70/2015-PPS.
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