Plant Protect. Sci., 2017, 53(1):32-43 | DOI: 10.17221/14/2016-PPS
Characterisation of endophytic bacteria from a desert plant Lepidium perfoliatum L.Original Paper
- Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, College of Life Science, Xinjiang Normal University, Xinjiang, P.R. China
Sixty-two endophytic bacteria from the leaves, roots, and stems of healthy Lepidium perfoliatum L. were isolated and characterised. From the results, 89, 87, 90, and 97% isolates could tolerate 12% NaCl, 30% PEG 6000, 50°C and pH 10, respectively. 74% isolates could form a biofilm. Besides, 28 isolates could improve the germination rate of host seeds under different degree of drought stress. These data suggest that the endophyte isolates show considerable resistance to abiotic stress and assist their plant hosts to germinate under drought stress.
Keywords: Lepidium perfoliatum L.; endophytic bacterial diversity; biofilm; salt tolerance; drought tolerance
Published: March 31, 2017 Show citation
References
- Ali S., Charles T.C., Glick B.R. (2014): Amelioration of high salinity stress damage by plant growth-promoting bacterial endophytes that contain ACC deaminase. Plant Physiology and Biochemistry, 80: 160-167.
Go to original source...
Go to PubMed...
- Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J. (1990): Basic local alignment search tool. Journal of Molecular Biology, 215: 403-410.
Go to original source...
Go to PubMed...
- Araújo W.L., Maccheroni Jr W., Aguilar-Vildoso C.I., Barroso P.A., Saridakis H.O., Azevedo J.L. (2001): Variability and interactions between endophytic bacteria and fungi isolated from leaf tissues of citrus rootstocks. Canadian Journal of Microbiology, 47: 229-236.
Go to original source...
Go to PubMed...
- Becker A. (2015): Challenges and perspectives in combinatorial assembly of novel exopolysaccharide biosynthesis pathways. Frontiers in Microbiology, 6, Article No. 687. doi: 10.3389/fmicb.2015.00687
Go to original source...
Go to PubMed...
- Castro R.A., Quecine M.C., Lacava P.T., Batista B.D., Luvizotto D.M., Marcon J., Ferreira A., Melo I.S., Azevedo J.L. (2014): Isolation and enzyme bioprospection of endophytic bacteria associated with plants of Brazilian mangrove ecosystem. SpringerPlus, 3.382. doi: 10.1186/2193-1801-3-382
Go to original source...
Go to PubMed...
- Chelius M.K., Triplett E.W. (2001): The diversity of archaea and bacteria in association with the roots of Zea mays L. Microbial Ecology, 41: 252-263.
Go to original source...
Go to PubMed...
- Cherif H., Marasco R., Rolli E., Ferjani R., Fusi M., Soussi A., Mapelli F., Blilou I., Borin S., Boudabous A., Cherif A., Daffonchio D., Ouzari H. (2015): Oasis desert farming selects environment-specific date palm root endophytic communities and cultivable bacteria that promote resistance to drought. Environmental Microbiology Reports, 7: 668-678.
Go to original source...
Go to PubMed...
- Costerton J.W., Lewandowski Z., Caldwell D.E., Korber D.R., Lappin-Scott H.M. (1995): Microbial biofilms. Annual Review of Microbiology, 49: 711-745.
Go to original source...
Go to PubMed...
- Costerton J.W., Stewart P.S., Greenberg E.P. (1999): Bacterial biofilms: a common cause of persistent infections. Science, 284: 1318-1322.
Go to original source...
Go to PubMed...
- Forchetti G., Masciarelli O., Alemano S., Alvarez D., Abdala G. (2007): Endophytic bacteria in sunflower (Helianthus annuus L.): isolation, characterization, and production of jasmonates and abscisic acid in culture medium. Applied Microbiology and Biotechnology, 76: 1145-1152.
Go to original source...
Go to PubMed...
- Forchetti G., Masciarelli O., Izaguirre M.J., Alemano S., Alvarez D., Abdala G. (2010): Endophytic bacteria improve seedling growth of sunflower under water stress, produce salicylic acid, and inhibit growth of pathogenic fungi. Current Microbiology, 61: 485-493.
Go to original source...
Go to PubMed...
- Fterich A., Mahdhi M., Lafuente A., Pajuelo E., Caviedes M.A., Rodriguez-Llorente I.D., Mars M. (2012): Taxonomic and symbiotic diversity of bacteria isolated from nodules of Acacia tortilis subsp. raddiana in arid soils of Tunisia. Canadian Journal of Microbiology, 58: 738-751.
Go to original source...
Go to PubMed...
- Fujishige N.A., Kapadia N.N., De Hoff P.L., Hirsch A.M. (2006): Investigations of Rhizobium biofilm formation. FEMS Microbiology Ecology, 56: 195-206.
Go to original source...
Go to PubMed...
- Fujishige N.A., Lum M.R., De Hoff P.L., Whitelegge J.P., Faull K.F., Hirsch A.M. (2008): Rhizobium common nod genes are required for biofilm formation. Molecular Microbiology, 67: 504-515.
Go to original source...
Go to PubMed...
- Hallmann J., Quadt-Hallmann A., Mahaffee W.F., Kloepper J.W. (2011): Bacterial endophytes in agricultural crops. Canadian Journal of Microbiology, 43: 895-914.
Go to original source...
- Hirsch A.M. (2010): How rhizobia survive in the absence of a legume host, a stressful world indeed. In: Seckbach J., Grube M. (eds): Symbioses and Stress. Joint Ventures in Biology. Dordrecht, Springer: 375-391.
Go to original source...
- Huang D.H., Zhao J., Yuan J.W., Dong-Sheng X.U., Chen S., Lan H.Y. (2011): A preliminary study on methods for dormancy breaking of seeds of Lepidium perfoliatum L. cultivated outdoor. Xinjiang Agricultural Sciences, 2011-03.
- Jha Y., Subramanian R.B., Patel S. (2011): Combination of endophytic and rhizospheric plant growth promoting rhizobacteria in Oryza sativa shows higher accumulation of osmoprotectant against saline stress. Acta Physiologiae Plantarum, 33: 797-802.
Go to original source...
- Karthikeyan B., Joe M.M., Islam M.R., Sa T.M. (2012): ACC deaminase containing diazotrophic endophytic bacteria ameliorate salt stress in Catharanthus roseus through reduced ethylene levels and induction of antioxidative defense systems. Symbiosis, 56: 77-86.
Go to original source...
- Krishnan P., Bhat R., Kush A., Ravikumar P. (2012): Isolation and functional characterization of bacterial endophytes from Carica papaya fruits. Journal of Applied Microbiology, 113: 308-317.
Go to original source...
Go to PubMed...
- Kumar S., Tamura K., Nei M. (2004): MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Briefings in Bioinformatics, 5: 150-163.
Go to original source...
Go to PubMed...
- Li-Li G.U., Liu L.H., You T.Y., Lan H.Y., Zhang F.C. (2008): Characterization of the seed coat mucilage properties of ephemeral plant Lepidium perfoliatum L. in Xinjiang. Acta Botanica Boreali-Occidentalia Sinica, 28: 2451- 2460. doi: 10.17221/14/2016-PPS
Go to original source...
- Meneses C.H., Rouws L.F., Simoes-Araujo J.L., Vidal M.S., Baldani J.I. (2011): Exopolysaccharide production is required for biofilm formation and plant colonization by the nitrogen-fixing endophyte Gluconacetobacter diazotrophicus. Molecular Plant-Microbe Interactions, 24: 1448-1458.
Go to original source...
Go to PubMed...
- Misaghi I.J., Donndelinger C.R. (1990): Endophytic bacteria in symptom-free cotton plants. Phytopathology, 80: 808-811.
Go to original source...
- Naveed M., Hussain M.B., Zahir Z.A., Mitter B., Sessitsch A. (2014a): Drought stress amelioration in wheat through inoculation with Burkholderia phytofirmans strain PsJN. Plant Growth Regulation, 73: 121-131.
Go to original source...
- Naveed M., Mitter B., Reichenauer T.G., Wieczorek K., Sessitsch A. (2014b): Increased drought stress resilience of maize through endophytic colonization by Burkholderia phytofirmans PsJN and Enterobacter sp. FD17. Environmental & Experimental Botany, 97: 30-39.
Go to original source...
- Qin S., Zhang Y.J., Yuan B., Xu P.Y., Xing K., Wang J., Jiang J.H. (2014): Isolation of ACC deaminase-producing habitat-adapted symbiotic bacteria associated with halophyte Limonium sinense (Girard) Kuntze and evaluating their plant growth-promoting activity under salt stress. Plant and Soil, 374: 753-766.
Go to original source...
- Qurashi A.W., Sabri A.N. (2012): Biofilm formation in moderately halophilic bacteria is influenced by varying salinity levels. Journal of Basic Microbiology, 52: 566-572.
Go to original source...
Go to PubMed...
- Reinhold-Hurek B., Hurek T. (2011): Living inside plants: bacterial endophytes. Current Opinion in Plant Biology, 14: 435-443.
Go to original source...
Go to PubMed...
- Rorth M., Jensen P.K. (1967): Determination of catalase activity by means of the Clark oxygen electrode. Biochimica et Biophysica Acta, 139: 171-173.
Go to original source...
Go to PubMed...
- Saitou N., Nei M. (1987): The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4: 406-425.
Go to PubMed...
- Sambrook J., Fritsch E.F., Maniatis T. (1989): Molecular Cloning: A Laboratory Manual. 2nd Ed. New York, CSH Laboratory Press.
- Seneviratne G., Weerasekara M.L.M.A.W., Seneviratne K.A.C.N., Zavahir J.S., Kecskés M.L., Kennedy I.R. (2010): Importance of biofilm formation in plant growth promoting rhizobacterial action. In: Maheshwari D.K. (ed.): Plant Growth and Health Promoting Bacteria. Vol. 18. Berlin, Springer: 81-95.
Go to original source...
- Sgroy V., Cassan F., Masciarelli O., Del Papa M.F., Lagares A., Luna V. (2009): Isolation and characterization of endophytic plant growth-promoting (PGPB) or stress homeostasis-regulating (PSHB) bacteria associated to the halophyte Prosopis strombulifera. Applied Microbiology and Biotechnology, 85: 371-381.
Go to original source...
Go to PubMed...
- Shi Y., Zhang X., Lou K. (2013): Isolation, characterization, and insecticidal activity of an endophyte of drunken horse grass, Achnatherum inebrians. Journal of Insect Science, 13: 151.
Go to original source...
Go to PubMed...
- Stanley N.R., Lazazzera B.A. (2004): Environmental signals and regulatory pathways that influence biofilm formation. Molecular Microbiology, 52: 917-924.
Go to original source...
Go to PubMed...
- Sturz A.V. (1995): The role of endophytic bacteria during seed piece decay and potato tuberization. Plant & Soil, 175: 257-263.
Go to original source...
- Tariq M., Hameed S., Yasmeen T., Zahid M., Zafar M. (2014): Molecular characterization and identification of plant growth promoting endophytic bacteria isolated from the root nodules of pea (Pisum sativum L.). World Journal of Microbiology & Biotechnology, 30: 719-725.
Go to original source...
Go to PubMed...
- Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G. (1997): The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25: 4876-4882.
Go to original source...
Go to PubMed...
- Xu Y.B., Chen M., Zhang Y., Wang M., Wang Y., Huang Q.B., Wang X., Wang G. (2014): The phosphotransferase system gene ptsI in the endophytic bacterium Bacillus cereus is required for biofilm formation, colonization, and biocontrol against wheat sharp eyespot. FEMS Microbiology Letters, 354: 142-152.
Go to original source...
Go to PubMed...
- Yuan J., Huang D., Dongsheng X.U., Zhao J., Ling L.I., Lan H., Zhang F. (2013): Cloning and analysis of seed coat mucilage-related gene MUM4 from Lepidium perfoliatum. Acta Botanica Boreali-Occidentalia Sinica, 33: 1940-1952.
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.