Plant Protect. Sci., 2018, 54(1):1-8 | DOI: 10.17221/18/2017-PPS

Pathogen causing Phalaenopsis soft rot disease - 16S rDNA and virulence characterisationOriginal Paper

Sudarsono Sudarsono1,2, Juanita Elina1,2, Giyanto3, Dewi Sukma*,2
1 PMB Lab., Department of Agronomy and Horticulture, Faculty of Agriculture, Bogor Agricultural University, Jl. Meranti ? Darmaga Campus, Bogor, West Java, Indonesia
2 PBT Program, IPB Graduate School, Bogor Agricultural University, Jl. Meranti ? Darmaga Campus, Bogor, West Java, Indonesia
3 Department of Plant Protection, Bogor Agricultural University, Jl. Meranti ? Darmaga Campus, Bogor, West Java, Indonesia

The pathogen causing Phalaenopsis soft rot disease and developed detached leaf inoculation methods were identified. Based on its 16S rDNA sequences, the pathogen causing soft rot disease in Phalaenopsis was Erwinia chrysanthemi/Dickeya chrysanthemi. Both virulent and avirulent strains were revealed. The detached leaf inoculation assay for E. chrysanthemi/D. chrysanthemi resistance evaluation included wounding and inoculating the detached leaf with 108 CFU/ml of bacteria. Soft rot disease symptoms in the inoculated detached leaf were measurable at 20 h after inoculation. The detached leaf assay was applicable for evaluating Phalaenopsis germplasm and progeny resistance in Phalaenopsis breeding programs.

Keywords: Erwinia, Dickeya chrysanthemi; moth orchids; detached leaf inoculation assay

Published: March 31, 2018  Show citation

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Sudarsono S, Elina J, Giyanto, Sukma D. Pathogen causing Phalaenopsis soft rot disease - 16S rDNA and virulence characterisation. Plant Protect. Sci. 2018;54(1):1-8. doi: 10.17221/18/2017-PPS.
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