Plant Protect. Sci., 2018, 54(2):101-110 | DOI: 10.17221/137/2016-PPS

Influence of selected fungicides registered in the Czech Republic for winter oilseed rape on in vitro Sclerotinia sclerotiorum mycelial growthOriginal Paper

Jana Poslušná*,1, Eva Plachká2, Jana Mazáková3
1 Plant Protection Department, Agritec Plant Research Ltd., Šumperk, Czech Republic
2 Research Institute of Oilseed Crops, OSEVA PRO, Ltd., Opava, Czech Republic
3 Department of Plant Protection, Faculty of agrobiology food and natural resources, Czech University of Life Sciences Prague, Prague, Czech Republic

The baseline sensitivity of 55 isolates of Sclerotinia sclerotiorum, collected from oilseed rape in 6 regions of the Czech Republic, to selected fungicides was determined during the period 2013-2015. One single-component fungicide - Horizon (tebuconazole), and four multicomponent fungicides - Pictor (boscalid, dimoxystrobin), Efilor (boscalid, metconazole), Prosaro 250 EC (prothioconazole, tebuconazole), and Propulse (fluopyram, prothioconazole), were chosen as these are commonly used locally. The effect of each fungicide on the in vitro pathogen radial mycelial growth and EC50 values for the respective fungicides were determined. The following MIC values were estimated; for the fungicides Horizon 250 EW, Efilor, and Propulse the mean MIC values ranged between 0.125 and 0.250 µl/ml, for Prosaro 250 EC ranged between 0.0625 and 0.125 µl/ml, and for Pictor ranged from 0.00781 to 0.01562 µl/ml. No strains of S. sclerotiorum resistant to the tested fungicides were detected and the growth of all isolates was fully inhibited at concentrations corresponding to their registered dose rates. The highest fungicidal efficacy on the collected S. sclerotiorum isolates was recorded for Pictor, followed by Prosaro 250 with an EC50 value 0.05856 µl/ml and then the remaining fungicides Propulse, Efilor, and Horizon 250 EW (EC50 values 0.07277, 0.07221, and 0.08519 µl/ml, respectively).

Keywords: Brassica napus L.; Sclerotinia sclerotiorum; efficacy; tebuconazole; boscalid; dimoxystrobin; metconazole; prothioconazole; fluopyram

Published: June 30, 2018  Show citation

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Poslušná J, Plachká E, Mazáková J. Influence of selected fungicides registered in the Czech Republic for winter oilseed rape on in vitro Sclerotinia sclerotiorum mycelial growth. Plant Protect. Sci. 2018;54(2):101-110. doi: 10.17221/137/2016-PPS.
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    References

    1. Bittner V. (2006): Škodlivé organismy řepky - abiotická poškození, choroby, škůdci. Hradec Kralové, Agrotisk.
    2. Boland G.J., Hall R. (1994): Index of plant hosts of Sclerotinia sclerotiorum. Canadian Journal of Plant Pathology, 16: 93-108. Go to original source...
    3. Brent K.J., Hollomon D.W. (2007): Fungicide Resistance in Crop Pathogens: How Can it be Managed? Brussels, Fungicide Resistance Action Committee.
    4. Caesar A.J., Pearson R.C. (1983): Environmental factors affecting survival of ascospores of Sclerotinia sclerotiorum. Phytopathology, 73: 1024-1030. Go to original source...
    5. ČSÚ (2016): Český statistický úřad. Zemědělství - časové řady. Available at www.czso.cz/csu/czso/zem_cr (accessed June 2, 2016).
    6. Dalili A., Bakhtuari S., Barari H., Aldaghi M. (2015): Effect of some fungicides against the growth inhibition of Sclerotinia sclerotiorum mycelial compatibility groups. Journal of Plant Protection Research, 55 (4). doi: 10.1515/jppr-2015-0047 Go to original source...
    7. Eckert M.R., Rossall S., Selley A., Fitt B.D.L. (2009): Effect of fungicides on in vitro spore germination and mycelial growth of the phytopathogens Lepthosphaeria maculans and L. biglobosa (phoma stem canker of oilseed rape). Pest Management Science, 66: 396-405. Go to original source... Go to PubMed...
    8. EU (2013): Regulation (EU) No 1307/2013 of the European Parliament and of the Council of 17 December 2013 establishing rules for direct payments to farmers under support schemes within the framework of the common agricultural policy and repealing Council Regulation (EC) No 637/2008 and Council Regulation (EC) No 73/2009. Official Journal of the European Union, L 347: 608-670.
    9. Fábry A. (2001): Rehabilitujeme význam osevního postupu pro pěstování ozimé řepky. Úroda. Available at http://uroda.cz/rehabilitujme-vyznam-osevniho-postupu-propestovani-ozime-repky (accessed June 2, 2016).
    10. FRAC (2013): Pathogen Risk List: Fungicidal Resistance Action Committee. Available at www.frac.info/docs/ default-source/publications/pathogen-risk/pathogenrisk-list.pdf?sfvrsn=8 (accessed July 6, 2016).
    11. FRAC (2016): FRAC Code List: Fungicides Sorted by Mode of Action (including FARC Code Numbering). FRAC.
    12. Gossen B.D., Rimmer S.R., Holley J.D. (2001): First report of resistance to benomyl fungicide in Sclerotinia sclerotiorum. Plant Disease, 85: 1206. Go to original source... Go to PubMed...
    13. Kazda J., Škeřík J. (eds) (2008): Metodika integrované ochrany řepky. Praha, SPZO s.r.o.
    14. Kuang J., Hou Y..P, Wang J.X., Zhou M.G. (2011): Sensitivity of Sclerotinia sclerotiorum to fludioxonil: in vitro determination of baseline sensitivity and resistance risk. Crop Protection, 30: 876-882. Go to original source...
    15. Li JL., Liu XY., Di YL., Liang HJ., Zhu FX. (2015): Baseline sensitivity and control efficacy of DMI fungicide epoxiconazole against Sclerotinia sclerotiorum. European Journal of Plant Pathology, 141: 237-246. Go to original source...
    16. Lobato K.R., Cardoso C.C., Binfaré R.W., Budni J., Wagner C.L.R., Brocardo P.S., de Souza L.F., Brocardo C., Flesch S., Freitas A.E., Dafré A.L., Rodrigues A.L.S. (2010): α-Tocopherol administration produces an antidepressant-like effect in predictive animal models of depression. Behaviour Brain Research, 209: 249-259. Go to original source... Go to PubMed...
    17. Ma H.X., Chen Y., Wang J.X., Yu W.Y., Tang Z.H., Chen C.J., Zhou M.G. (2009): Activity of carbendazim, dimethachlon, iprodione, procymidone and boscalid against Sclerotinia stem rot in Jiangsu Province of China. Phytoparasitica, 37: 421-429. Go to original source...
    18. Odstrčilová L. (2007): Choroby kmínu kořenného a možnosti ochrany proti nim. [Ph.D. Dissertation.] Brno, Mendel University in Brno.
    19. Pandey D.K., Tripathi N.N., Tripathi R.D., Dixit S.N. (1982): Fungitoxic and phytotoxic properties of the essential oil of Hyptis suaveolens. Zeitschrift für Pflanzenkrankheit und Pflanzenschutz, 89: 344-349.
    20. Paul V.H. (2003): RAPS - Krankheiten, Schädlinge, Schadpflanzen. Bonn, AgroConcept GmbH.
    21. Penaud A., Huguet B., Wilson V., Leroux P. (2003): Fungicide resistance of Sclerotinia sclerotiorum in French oilseed rape crops. In: Proceedings 11th International Rapeseed Congress, July 6-10, 2003, Copenhagen, Denmark: 6-10.
    22. Plachká E., Poslušná J. (2015): Zdravotní stav řepky olejky ozimé na Opavsku a Šumpersku v sezóně 2014/2015. In: Vyhodnocovací seminář Systém výroby řepky a Systém výroby slunečnice, Nov 25-26, 2015, Hluk, Česká republika. Praha, Svaz pěstitelů a zpracovatelů olejnin, s.r.o.: 73-81.
    23. Poslušná J., Plachká E. (2014): Možnosti ochrany vůči bílé hnilobě řepky. Farmář, 20 (5): 26-28.
    24. Purdy L.H. (1979): Sclerotinia sclerotiorum: history, diseases and symptomatology, host range, geographic distribution, and impact. Phytopathology, 69: 875-880. Go to original source...
    25. Spitzer T., Matušinský M., Klemová Z., Kazda J. (2012): Effect of fungicide application date against Sclerotinia sclerotiorum on yield and greening of winter rape. Plant Protectection Science, 48: 105-109. Go to original source...
    26. Šaroun J., Říha K. (2008): Možné příčiny poklesu výnosu řepky v ročníku 2007-2008. In: Sborník referátů 25. vyhodnocovacího semináře, Nov 20-21, 2008, Hluk, Česká republika. Praha, SPZO s.r.o., Svaz pěstitelů a zpracovatelů olejnin: 296-304.
    27. ÚKZÚZ (2016): Seznam povolených přípravků a dalších prostředků na ochranu rostlin 2016. Praha, Česká společnost rostlinolékařská.
    28. Xu D., Pan Y., Zhang H., Li X., Dai Y., Cao S., Gao Z. (2015): Detection and characterization of carbendazim resistance in Sclerotinia sclerotiorum isolates from oilseed rape in Anhui Province of China. Genetics and Molecular Research, 14: 16627-16638. Go to original source... Go to PubMed...
    29. Wang Y., Duan Y.B., Zhou M.G. (2015): Molecular and biochemical characterization of boscalid resistance in laboratory mutants of Sclerotinia sclerotiorum. Plant Pathology, 64: 101-108. Go to original source...
    30. Zhang C.Q., Yuan S.K, Sun H.Y., Qi Z.Q., Zhou M.G., Zhu G.N. (2007): Sensitivity of Botrytis cinerea from vegetable greenhouses to boscalid. Plant Pathology, 56: 646-53. Go to original source...
    31. Zhao J., Peltier A.J., Meng J., Osborn T.C., Grau C.R. (2004): Evaluation of Sclerotinia stem rot resistence in oilseed Brassica napus using a petiole inoculation technique under greenhouse conditions. Plant Disease, 88: 1033-1039. Go to original source... Go to PubMed...
    32. Zhou F., Zhang X.L., Li J.L., Zhu, F.X. (2014): Dimethachlon resistance in Sclerotinia sclerotiorum in China. Plant Disease, 98: 1221-1226. Go to original source... Go to PubMed...

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