Plant Protect. Sci., 2018, 54(2):111-117 | DOI: 10.17221/45/2017-PPS

Pathogenicity of Beauveria bassiana strain 202 against sap-sucking insect pestsOriginal Paper

Ghulam Ali Bugti1, Wang Bin1,*, Cao Na1, Lin Hua Feng2
1 Anhui Provincial Key Laboratory of Microbial Control and
2 School of Plant Protection, Anhui Agricultural University, Hefei, P.R. China

An experimental trial was conducted to determine the pathogenicity of Beauveria bassiana strain 202 (Bb-202) against multiple targeted sucking insect species that are serious pests of crops and ornamental plants. The insect species, Myzus persicae Sulzer (Hemiptera: Aphididae), Jacobiasca formosana Paoli (Hemiptera: Cicadellidae), Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae), and Stephanitis nashi (Hemiptera: Tingidae) were exposed to conidia of B. bassiana at rates of 1.0 × 102, 3.5 × 103, 5 × 104, and 6.75 × 105 conidia/mm2 to determine the pathogenicity of B. bassiana. The fungal strain Bb-202 showed the highest pathogenicity to M. persicae and 100% corrected mortality observed in the treatments over 1.0 × 102 conidia/mm2, followed by the J. formosana with the final corrected mortality of 86.6, 94.4, and 97.4% after 10 days of fungal inoculation with 3.5 × 103, 5 × 104, and 6.75 × 105 conidia/mm2, respectively. The strain Bb-202 also showed good pathogenicity to B. tabaci and corrected mortalities of 77.9 and 81.1% were recorded when exposed to 5 × 104 and 6.75 × 105 conidia/mm2. Relatively weak pathogenicity was observed in S. nashi, in which the highest corrected mortality of 63.7% occurred at 6.75 × 105 conidia/mm2. Accordingly, the LC50 and LT50 values of concentrations 1 × 105, 1 × 106, 1 × 107, and 1 × 108 conidia/ml were calculated for M. persicae, J. formosana, B. tabaci, and S. nashi that were determined as 6.7 × 104, 1.3 × 106, 3.6 × 106, and 1.2 × 107 conidia/ml and LT50 was observed as 5.2~8.24, 5.1~6.6, 7.2~9.0, and 7.9~9.6 in days, respectively.

Keywords: entomopathogenic fungi; Beauveria bassiana; plant sucking insects

Published: June 30, 2018  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Bugti GA, Bin W, Na C, Feng LH. Pathogenicity of Beauveria bassiana strain 202 against sap-sucking insect pests. Plant Protect. Sci. 2018;54(2):111-117. doi: 10.17221/45/2017-PPS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Alves M., Boscolo M., Fernandes O.A., Nunes M.A. (2008): Mortality of Bemisia tabaci biotype B (Sternorrhyncha: Aleyrodidae) adults by aliphatic and aromatic synthetic sucrose esters. Brazilian Archives of Biology & Technology, 51: 1115-1119. Go to original source...
    2. Askary H., Yarmand H. (2007): Development of the entomopathogenic hyphomycete Lecanicillium muscarium (Hyphomycetes: Moniliales) on various hosts. European Journal of Entomology, 104: 67-72. Go to original source...
    3. Balakrishnan N., Murugesan N., Vanniarajan C., Ramalingam A., Suriachandraselvan M. (2007): Screening of cotton genotypes for resistance to leafhopper, Amrasca biguttula biguttula Ishida in Tamil Nadu. Journal of Cotton Research & Development: 120-121.
    4. Biswas C., Dey P., Gotyal B.S., Satpathy S. (2015): A method of multiplex pcr for detection of field released Beauveria bassiana, a fungal entomopathogen applied for pest management in jute (Corchorus olitorius). World Journal of Microbiology and Biotechnology, 31: 1-5. Go to original source... Go to PubMed...
    5. Cao H.H., Liu H.R., Zhang Z.F., Liu T.X. (2015): The green peach aphid Myzus persicae perform better on pre-infested chinese cabbage Brassica pekinensis by enhancing host plant nutritional quality. Scientific Reports, 6: 21954. doi: 10.1038/srep21954 Go to original source... Go to PubMed...
    6. Decourtye A., Devillers J. (2010): Ecotoxicity of Neonicotinoid Insecticides to Bees. New York, Springer. Go to original source...
    7. El K.H., Devine G.J. (2003): Insecticide resistance in Egyptian populations of the cotton whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae). Pest Management Science, 59: 865-871. Go to original source... Go to PubMed...
    8. Elif T., Ali S., Zihni D., Mahmut E., Ismail D. (2010): Isolation and virulence of entomopathogenic fungi against the great spruce bark beetle, Dendroctonus micans (Kugelann) (Coleoptera: Scolytidae). Biocontrol Science and Technology, 20: 695-701. Go to original source...
    9. Elnaggar J.B., Zidan E.H.A. (2013): Field evaluation of imidacloprid and thiamethoxam against sucking insects and their side effects on soil fauna. Journal of Plant Protection Research, 53: 375-387. Go to original source...
    10. Fu J.Y., Han B.Y., Xiao Q. (2014): Mitochondrial coi and 16s RNA evidence for a single species hypothesis of E. vitis, J. formosana and E. onukii in East Asia. PloS One 9 (12): e115259. doi: 10.1371/journal.pone.0115259 Go to original source... Go to PubMed...
    11. Glare T.R., Milner R.J. (1991): Ecology of entomopathogenic fungi. In: Handbook of Applied Mycology Humans Animals and Insects. Vol. 2. New York, Dekker: 547-612.
    12. Godfray H.C.J., Blacquière T., Field L.M., Hails R.S., Petrokofsky G., Potts S.G., Raine N.E., Vanbergen A.J., McLean A.R. (2014): A restatement of the natural science evidence base concerning neonicotinoid insecticides and insect pollinators. Proceedings of the Royal Society B: Biological Sciences, 281 (1786). doi: 10.1098/rspb.2014.0558 Go to original source... Go to PubMed...
    13. Goettel M.S., Inglis G.D., Wraight S.P. (2000): Fungi. Dordrecht, Springer. Go to original source...
    14. Hou M., Liu Y., Wang B. (2015): Laboratory assessment on virulence of entomogenous fungi against Stephanitis nashi. Chinese Journal of Biological Control, 31: 853-859.
    15. Humber R.A. (1991): Fungal pathogens of aphids. In: Proceedings of an Osu Centennial Event: 45-56.
    16. Jaronski S.T. (2010): Ecological factors in the inundative use of fungal entomopathogens. Biocontrol, 55: 159-185. Go to original source...
    17. Khetan S.K. (2001): Microbial pest control. Journal of Phytopathology, 149: 491-492. https://doi.org/10.17221/45/2017-PPS Go to original source...
    18. Lacey L.A., Frutos R., Kaya H.K., Vail P. (2001): Insect pathogens as biological control agents: do they have a future? Biological Control, 21: 230-248. Go to original source...
    19. Li M.Y., Li S.G., Xu A., Lin H.F., Chen D.X., Wang H. (2014): Selection of Beauveria isolates pathogenic to adults of Nilaparvata lugens. Journal of Insect Science, 14: 32. Go to original source...
    20. Leger R.J.S., Roberts D.W. (1997): Engineering improved mycoinsecticides. Trends in Biotechnology, 15: 83-85. Go to original source...
    21. Loc N.T., Chi V.T.B., Hung P.Q., Thi N. (2002): Effect of Beauveria bassiana and Metarhizium anisopliae on some natural enemies of rice insect pests. Science & Technology Journal of Agriculture & Rural Development, 2002: 490-493..
    22. Long D.W., Drummond F.A., Groden E., Donahue D.W. (2000): Modelling Beauveria bassiana horizontal transmission. Agricultural and Forest Entomology, 2: 19-32. Go to original source...
    23. Ma D., Gorman K., Devine G., Luo W., Denholm I. (2007): The biotype and insecticide-resistance status of whiteflies, Bemisia tabaci (Hemiptera: Aleyrodidae), invading cropping systems in Xinjiang Uygur Autonomous Region, northwestern China. Crop Protection, 26: 612-617. Go to original source...
    24. Mahmoud M.F. (2009): Pathogenicity of three commercial products of entomopathogenic fungi, Beauveria bassiana, Metarhizum anisopilae and Lecanicillium lecanii against adults of olive fly, Bactrocera oleae (Gmelin) (Diptera: Tephritidae) in the laboratory. Plant Protection Science, 45: 98-102. Go to original source...
    25. Mithöfer A., Boland W. (2008): Recognition of herbivory-associated molecular patterns. Plant Physiology, 146: 825-831. Go to original source... Go to PubMed...
    26. Mohammadbeigi A.,Port G. (2013): Efficacy of Beauveria bassiana and Metarhizium anisopliae against Uvarovistia zebra (Orthoptera: Tettigoniidae) via contact and ingestion. International Journal of Agriculture and Crop Sciences, 5: 138-146.
    27. Poprawski T.J., Parker P.E., Tsai J.H. (1999): Laboratory and field evaluation of hyphomycete insect pathogenic fungi for control of brown citrus aphid (Homoptera: Aphididae). Environmental Entomology, 28: 315-321. Go to original source...
    28. Riosvelasco C., Pérezcorral D.A., Salasmarina M.Á., Berlangareyes D.I., Ornelaspaz J.J., Muñiz C.H.A., Camberocampos J., Jacobocuellar J.L. (2009): Pathogenicity of the hypocreales fungi Beauveria bassiana and Metarhizium anisopliae against insect pests of tomato. Southwestern Entomologist, 39: 739-750. Go to original source...
    29. Regan K., Ordosch D., Glover K.D., Tilmon K.J., Szczepaniec A. (2017): Effects of a pyrethroid and two neonicotinoid insecticides on population dynamics of key pests of soybean and abundance of their natural enemies. Crop Protection, 98: 24-32. Go to original source...
    30. Roy H.E., Cottrell T.E. (2008): Forgotten natural enemies: interactions between coccinellids and insect-parasitic fungi. European Journal of Entomology, 105: 391-398. Go to original source...
    31. Schmidtjeffris R.A., Nault B.A. (2016): Anthranilic diamide insecticides delivered via multiple approaches to control vegetable pests: a case study in snap bean. Journal of Entomology, Tow 219. doi: 10.1093/jee/tow219 Go to original source... Go to PubMed...
    32. Selvaraj K., Kaushik H.D., Gulati R., Sharma S.S. (2012): Evaluation of Beauveria bassiana (Balsamo) Vuillemin against Aphis craccivora (Koch) (Aphididae: Homoptera). Biopesticides International, 8: 125-130.
    33. Sevim A., Donzelli B.G., Wu D., Demirbag Z., Gibson D. M., Turgeon B.G. (2012): Hydrophobin genes of the entomopathogenic fungus, Metarhizium brunneum, are differentially expressed and corresponding mutants are decreased in virulence. Current Genetics, 58: 79-92. Go to original source... Go to PubMed...
    34. Sevim A., Demir I., Sönmez E., Kocaçevik S., Demirbağ Z. (2013): Evaluation of entomopathogenic fungi against the sycamore lace bug, Corythucha ciliata (Say) (Hemiptera: Tingidae). Turkish Journal of Agriculture & Forestry, 37: 595-603. Go to original source...
    35. Shang Y., Feng P., Wang C. (2015): Fungi that infect insects: altering host behavior and beyond. Plos Pathogens, 11(8): e1005037. doi: 10.1371/journal.ppat.1005037 Go to original source... Go to PubMed...
    36. Todorova S.I., Cloutier C., Côté J.C., Coderre D. (2002): Pathogenicity of six isolates of Beauveria bassiana (Balsamo) Vuillemin (Deuteromycotina, Hyphomycetes) to Perillus bioculatus (F) (Hem., Pentatomidae). Journal of Applied Entomology, 126: 182-185. Go to original source...
    37. Varghese T.S., Mathew T.B. (2013): Bioefficacy and safety evaluation of newer insecticides and acaricides against chilli thrips and mites. Journal of Tropical Agriculture, 51: 111-115.
    38. Wu S., Gao Y., Zhang Y., Wang E., Xu X., Lei Z. (2014): An entomopathogenic strain of Beauveria Bassiana against Frankliniella occidentalis with no detrimental effect on the predatory mite neoseiulus barkeri: evidence from laboratory bioassay and scanning electron microscopic observation. PloS One, 9: E84732. doi: 10.1371/journal.pone.0084732 Go to original source... Go to PubMed...
    39. Wu S., Gao Y., Smagghe G., Xu X., Lei Z. (2016): Interactions between the entomopathogenic fungus Beauveria bassiana and the predatory mite Neoseiulus barkeri and biological control of their shared prey/host Frankliniella occidentalis. Biological Control, 98: 43-51. Go to original source...
    40. Zafar J., Freed S., Khan B.A., Farooq M. (2016): Effectiveness of Beauveria bassiana against cotton whitefly, Bemisia tabaci (Gennadius) (Aleyrodidae: Homoptera) on different host plants. Pakistan Journal of Zoology, 48: 91-99.
    41. Zimmermann G. (2007): Review on safety of the entomopathogenic fungi Beauveria bassiana and Beauveria brongniartii. Biocontrol Science and Technology, 17: 553-596. Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content