Plant Protect. Sci., 2020, 56(4):317-328 | DOI: 10.17221/103/2019-PPS

Investigation of some biochemical mechanisms involved in the resistance of faba bean (Vicia faba L.) varieties to Orobanche spp.Original Paper

Zouhaier Abbes*,1, Amal Bouallegue2, Imen Trabelsi3, Najla Trabelsi4, Amani Taamalli5, Moez Amri6, Haythem Mhadhbi2, Mohamed Kharrat1
1 Université de Carthage, Laboratoire des Grandes Cultures, Institut National de la Recherche Agronomique de Tunisie (INRAT), El Menzah, Tunisie
2 Centre de Biotechnologie de Borj Cédria (CBBC), Laboratoire des Légumineuses, Hammam Lif, Tunisie
3 Université de Carthage, Laboratoire des Sciences et Techniques Agronomiques, Institut National de la Recherche Agronomique de Tunisie (INRAT), El Menzah, Tunisie
4 Centre de Biotechnologie de Borj Cédria (CBBC), Laboratoire de Biotechnologie de l'Olivier, Hammam Lif, Tunisie
5 Department of Chemistry, College of Sciences, University of Hafr Al Batin, Saudi Arabia
6 AgroBioSciences Research program, University Mohammed VI Polytechnic Benguerir, Marrakech, Morocco

Broomrapes are weedy root parasitic plants that cause important damage to the faba bean production. Genetic resistance is considered as the most desirable control method. In this study, we investigated some of the mechanisms involved in the resistance to Orobanche crenata and O. foetida for two Tunisian resistant varieties Najeh and Chourouk in comparison with the susceptible variety Badi. The results showed that, for Najeh and Chourouk, the Orobanche infestation did not significantly influence the plant growth as indicated by inconsiderable changes in the shoot and root dry weights, pod setting and chlorophyll and carotenoid contents. In comparison to Badi, both resistant varieties showed a reduced Orobanche attachments/tubercles number and dry weights, low H2O2 and malondialdehyde (MDA) accumulation with increased levels of peroxidase activity in the roots. An Orobanche infestation was found to induce a significant decrease in the total lipid content and lipid unsaturation in the Badi leaves only. For resistant varieties, no significant changes were observed in these two parameters, which may indicate conservation and stability in the membrane fluidity. The resistance of Najeh and Chourouk was mainly associated with a relatively more efficient enzymatic antioxidative response and reduced lipid peroxidation that helped the plants to avoid the damaging effect of an Orobanche attack. Therefore, these two varieties could be used as a component of an integrated control strategy to improve the plant growth and productivity under an Orobanche infestation

Keywords: antioxidant; fatty acids; broomrapes; genetic resistance

Published: December 31, 2020  Show citation

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Abbes Z, Bouallegue A, Trabelsi I, Trabelsi N, Taamalli A, Amri M, et al.. Investigation of some biochemical mechanisms involved in the resistance of faba bean (Vicia faba L.) varieties to Orobanche spp. Plant Protect. Sci. 2020;56(4):317-328. doi: 10.17221/103/2019-PPS.
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