Plant Protect. Sci., 2021, 57(1):14-20 | DOI: 10.17221/1/2020-PPS

Assessing BactoMix 5 efficacy for clubroot control in naturally infested soilOriginal Paper

Kaire Loit, Riinu Kiiker ORCID...*, Britt Puidet, Liina Soonvald, Marian Põldmets, Marika Mänd
Chair of Plant Health, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Tartu, Estonia

The cultivation of cruciferous crops is threatened by extensive yield losses caused by the soil-borne pathogen Plasmodiophora brassicae Woronin, 1877. The objective of the study was to assess the potential of the bacterial product BactoMix 5 for the control of clubroot on a naturally infested soil in growth chamber trials using a P. brassicae-specific qPCR methodology. The results did not show a significant decrease in the P. brassicae in the soil nor a reduction of the disease symptoms on the plants. The native soil microbiota may have exhibited an antagonistic activity against the bacterial species from BactoMix 5 and evoked the poor effect of the product. Therefore, potential biological control agents should be tested with native soil microbiota and the regional production should be advanced to increase the product efficacy in the environment

Keywords: Plasmodiophora brassicae; Brassica; biocontrol; biostimulant

Published: December 3, 2020  Show citation

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Loit K, Kiiker R, Puidet B, Soonvald L, Põldmets M, Mänd M. Assessing BactoMix 5 efficacy for clubroot control in naturally infested soil. Plant Protect. Sci. 2021;57(1):14-20. doi: 10.17221/1/2020-PPS.
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    References

    1. Almquist C. (2016): Monitoring important soil-borne plant pathogens in Swedish crop production using real-time PCR. [PhD Thesis]. Upsala, Swedish University of Agricultural Sciences: 78.
    2. CABI (2019): Invasive Species Compendium. Datasheet: Plasmodiophora brassicae (club root). Available at https://www.cabi.org/isc/datasheet/41865 (accesed March 28, 2019).
    3. Cao T., Rennie D.C., Manolii V.P., Hwang S.F., Falak I., Strelkov S.E. (2014): Quantifying resistance to Plasmodiophora brassicae in Brassica hosts. Plant Pathology, 63: 715-726. Go to original source...
    4. Castlebury, L.A., Maddox J.V., Glawe D.A. (1994): A technique for the extraction and purification of viable Plasmodiophora brassicae resting spores from host root tissue. Mycologia, 86: 458-460. Go to original source...
    5. Dixon G.R., Page L.V. (1998): Calcium and nitrogen eliciting alterations to growth and reproduction of Plasmodiophora brassicae (clubroot). Acta Horticulturae, 459: 343-349. Go to original source...
    6. Dixon G.R. (2009): The occurrence and economic impact of Plasmodiophora brassicae and clubroot disease. Journal of Plant Growth Regulation, 28: 194-202. Go to original source...
    7. Donald C., Lawrence J.M., Porter I. (2004): Influence of particle size and application method on the efficacy of calcium cyanamide for control of clubroot of vegetable brassicas. Crop Protection, 23: 297-303. Go to original source...
    8. Donald C., Porter I. (2009): Integrated control of clubroot. Journal of Plant Growth Regulation, 28: 289-303. Go to original source...
    9. Einhorn G., Bochow H., Huber J., Krebs B. (1991): Methodological studies on the detection of antagonists of the clubroot pathogen Plasmodiophora brassicae Wor. Archive für Phytopathologie und Pflanzenschutz, 27: 205-208. Go to original source...
    10. Faggian R., Strelkov S.E. (2009): Detection and measurement of Plasmodiophora brassicae. Journal of Plant Growth Regulation, 28: 282-288. Go to original source...
    11. Gao Y., Xu, G. (2014): Development of an effective nonchemical method against Plasmodiophora brassicae on Chinese cabbage. International Journal of Agronomy, 2014: 1-5. Go to original source...
    12. Guetsky R., Shtienberg D., Elad Y., Fischer E., Dinoor A. (2002): Improving biological control by combining biocontrol agents each with several mechanisms of disease suppression. Phytopathology, 92: 976-985. Go to original source... Go to PubMed...
    13. Handelsman J., Stabb E.V. (1996): Biocontrol of soilborne plant pathogens. The Plant Cell, 8: 1855-1869. Go to original source... Go to PubMed...
    14. He P., Cui W., Munir S., He P., Li X., Wu Y., Yang X., Tang P., He Y. (2019): Plasmodiophora brassicae root hair interaction and control by Bacillus subtilis XF-1 in Chinese cabbage. Biological Control, 28: 56-63. Go to original source...
    15. Hwang S., Strelkov S.E., Feng J., Gossen B. D., Howard R.J. (2012): Plasmodiophora brassicae: a review of an emerging pathogen of the Canadian canola (Brassica napus) crop. Molecular Plant Pathology, 13: 105-113. Go to original source... Go to PubMed...
    16. Kuginuki Y., Hiroaki Y., Hirai M. (1999): Variation in virulence of Plasmodiophora brassicae in Japan tested with clubroot-resistant cultivars of Chinese cabbage (Brassica rapa L. spp. pekinensis). European Journal of Plant Pathology, 105: 327-332. Go to original source...
    17. Kumar S. (2012): Biopesticides: a need for food and environmental safety. Journal of Biofertilizers and Biopesticides, 3: e107. doi: 10.4172/2155-6202.1000e107 Go to original source...
    18. Kurowski T.P., Majchrzak B., Kowalska E. (2009): The effectiveness of the biological control of clubroot (Plasmodiophora brassicae) in Brassicaceae plants. Phytopathologia, 52: 5-12.
    19. Lahlali R., Peng G., Gossen B.D., McGregor L., Yu F.Q., Hynes R.K., Hwang S.F., McDonald M.R., Boyetchko S.M. (2013): Evidence that the biofungicide Serenade (Bacillus subtilis) suppresses clubroot on canola via antibiosis and induced host resistance. Phytopathology, 103: 245-254. Go to original source... Go to PubMed...
    20. Lengai G., Muthomi J. (2018): Biopesticides and their role in sustainable agricultural production. Journal of Biosciences and Medicines, 6: 7-41. Go to original source...
    21. Mehnaz S. (2013): Secondary metabolites of Pseudomonas aurantiaca and their role in plant growth promotion. In: Arora N.K. (ed.): Plant Microbe Symbiosis: Fundamentals and Advances. New Delhi, Springer: 373-393. Go to original source...
    22. O'Brien P. (2017): Biological control of plant diseases. Australasian Plant Pathology, 46: 293-304. Go to original source...
    23. Pal K.K., McSpadden Gardener B. (2006): Biological control of plant pathogens. The Plant Health Instructor. doi: 10.1094/PHI-A-2006-1117-02 Go to original source...
    24. Panda A.K, Bisht S.S., DeMondal S., Senthil Kumar N., Gurusubramanian G., Panigrahi A.K. (2014): Brevibacillus as a biological tool: a short review. Antonie Van Leeuwenhoek, 105: 623-639. Go to original source... Go to PubMed...
    25. Peng G., Lahlali R., Hwang S.F., Pageau D., Hynes R.K., Mc-Donald M.R., Gossen B.D., Strelkov S.E. (2014): Crop rotation, cultivar resistance, and fungicides/biofungicides for managing clubroot (Plasmodiophora brassicae) on canola. Canadian Journal of Plant Pathology, 36: 99-112. Go to original source...
    26. Rennie D.C, Manolii V.P., Cao T., Hwang S.F., Howard R.J., Strelkov S.E. (2011): Direct evidence of surface infestation of seeds and tubers by Plasmodiophora brassicae and quantification of spore load. Plant Pathology, 60: 811-819. Go to original source...
    27. Øíèaøová V., Kazda J., Singh K., Ry¹ánek P. (2016): Clubroot caused by Plasmodiophora brassicae Wor.: a review of emerging serious disease of oilseed rape in the Czech Republic. Plant Protection Science, 52: 71-86. Go to original source...
    28. Ruijter J.M., Ramakers C., Hoogaars W.M. H., Karlen Y., Bakker O., van den Hoff M.J.B., Moorman A.F.M. (2009): Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data. Nucleic Acids Research, 37: e45. doi: 10.1093/nar/gkp045 Go to original source... Go to PubMed...
    29. SCANDAGRA (2019): BactoMix 5. Available at https://https://scandagra.ee/tooted/bactomix-5/ (accessed March 20, 2019).
    30. Singh H.B. (2014): Management of plant pathogens with microorganisms. Proceedings of the Indian National Science Academy, 2: 443-454. Go to original source...
    31. Todero I., Confortin T.C., Luft L., Brun T., Ugalde G.A., de Almeida T.C., Arnemann J.A., Zabot G.L., Mazutti M.A. (2018): Formulation of a bioherbicide with metabolites from Phoma sp. Scientia Horticulturae, 241: 285-292. Go to original source...
    32. Vinale F., Sivasithamparam K., Ghisalberti E., Marra R., Barbetti M.J., Li H., Woo S., Lorito M. (2008): A novel role for The Trichoderma-plant interaction Trichoderma secondary metabolites in the interactions with plants. Physiological and Molecular Plant Pathology, 72: 80-86. Go to original source...
    33. Walker R., Powell A.A., Seddon B. (1998): Bacillus isolates from the spermosphere of peas and dwarf French beans with antifungal activity against Botrytis cinerea and Pythium species. Journal of Applied Microbiology, 84: 794-801. Go to original source... Go to PubMed...
    34. Wallenhammar A.C. (1996): Prevalence of Plasmodiophora brassicae in a spring oilseed rape growing area in central Sweden and factors influencing soil infestation levels. Plant Pathology, 45: 710-719. Go to original source...
    35. Wallenhammar A., Almquist C., Söderström M., Jonsson A. (2012): In-field distribution of Plasmodiophora brassicae measured using quantitative real-time PCR. Plant Pathology, 61: 16-28. Go to original source...
    36. Wallenhammar A.C., Gunnarson A., Hansson F., Jonsson A. (2016): Quantification of Plasmodiophora brassicae using a DNA-based soil test facilitates sustainable oilseed rape production. Plants (Basel), 5:21. doi: 10.3390/plants5020021 Go to original source... Go to PubMed...
    37. Xu X.-M., Jeffries P., Pautasso M., Jeger M.J. (2011): Combined use of biocontrol agents to manage plant diseases in theory and practice. Phytopathology, 101: 1024-1031. Go to original source... Go to PubMed...

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