Plant Protect. Sci., 2021, 57(1):66-69 | DOI: 10.17221/68/2020-PPS

Diplodia sapinea in Swedish forest nurseriesShort Communication

Rebecca Larsson ORCID...*, Audrius Menkis ORCID..., Åke Olson ORCID...
Department of Forest Mycology & Plant Pathology, Uppsala BioCenter, Swedish University of Agricultural Sciences, Uppsala, Sweden

Diplodia sapinea is a common forest pathogen on Pinus spp. in a large part of the world. In 2013, disease caused by this pathogen on Scots pine (Pinus sylvestris) trees in Sweden was reported for the first time. In this study, we report the first detection of D. sapinea on diseased seedlings of P. sylvestris from two Swedish forest nurseries. Infected seedlings were collected July-November 2019. Diplodia sapinea was identified by morphological characteristics of fungal structures on plant tissues and from culture grown on Hagem agar media, followed by sequencing of fungal ITS rDNA. The result emphasizes the susceptibility of P. sylvestris seedlings. More research is needed to better understand the risk for disease spreading within forest nurseries and into the forest through infected plant material.

Keywords: fungal disease; ITS rDNA; pathogen; Pinus sylvestris; pine seedlings

Published: December 3, 2020  Show citation

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Larsson R, Menkis A, Olson Å. Diplodia sapinea in Swedish forest nurseries. Plant Protect. Sci. 2021;57(1):66-69. doi: 10.17221/68/2020-PPS.
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References

  1. Brodde L., Adamson K., Camarero J., Castano C., Drenkhan R., Lehtijärvi A., Luchi N., Migliorini D., Sanchez-Miranda A., Stenlid J., Özdag S., Oliva J. (2019): Diplodia tip blight on its way to the north: drivers of disease emergence in Northern Europe. Frontiers in Plant Science, 9: 1-12. Go to original source... Go to PubMed...
  2. Menkis A., Vasiliauskas R., Taylor A.F.S., Stenlid J., Finlay R. (2005): Fungal communities in mycorrhizal roots of conifer seedlings in forest nurseries under different cultivation systems, assessed by morphotyping, direct sequencing and mycelial isolation. Mycorrhiza, 16: 33-41. Go to original source... Go to PubMed...
  3. Molin N., Persson M., Persson S. (1961): Root parasites on forest tree seedlings. Some exploratory tests of the resistance of germinant seedlings and the virulence of some potential parasites. Meddelande Från Statens Skogsforskningsinstitut, 49: 1-16.
  4. Oliva J., Boberg J., Stenlid J. (2013): First report of Sphaeropsis sapinea on Scots pine (Pinus sylvestris) and Austrian pine (P. nigra) in Sweden. New Disease Reports, 27: 23. doi: 10.5197/j.2044-0588.2013.027.023 Go to original source...
  5. Phillips A.J.L., Alves A., Abdollahzadeh J., Slippers B., Wingfield M.J., Groenewald J.Z., Crous P.W. (2013): The Botryosphaeriaceae: genera and species known from culture. Studies in Mycology, 76: 51-167. Go to original source... Go to PubMed...
  6. Smith D.R., Stanosz G.R. (2006): A species-specific PCR assay for detection of Diplodia pinea and D. scrobiculata in dead red and jack pines with collar rot symptoms. Plant Disease, 90: 307-313. Go to original source... Go to PubMed...
  7. Stanosz G.R., Smith D.R., Leisso R. (2007): Diplodia shoot blight and asymptomatic persistence of Diplodia pinea on or in stems of jack pine nursery seedlings. Forest Pathology, 37: 145-154. Go to original source...
  8. Stenlid J. (1985). Population structure of Heterobasidion annosum as determined by somatic incompatibility, sexual incompatibility, and isoenzyme patterns. Canadian Journal of Botany, 63: 2268-2273. Go to original source...

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