Plant Protect. Sci., 2021, 57(3):196-205 | DOI: 10.17221/140/2020-PPS

Epidemiology studies of Pseudomonas syringae pathovars associated with bacterial canker on the sweet cherry in SerbiaOriginal Paper

Renata Iličić*,1, Jelica Balaž1, Vladislav Ognjanov1, Tatjana Popović2
1 Department for Environmental and Plant Protection, Faculty of Agriculture, University of Novi Sad, Sad, Serbia
2 Institute for Plant Protection and Environment, Belgrade, Serbia

This study was conducted to examine the epidemiological characteristics of two Pseudomonas syringae pvs. - syringae (Pss) and morsprunorum race 1 (Psm) on the sweet cherry in both field and laboratory conditions. Cycled inoculations of one-year-old branches indicated that the sweet cherry became sensitive to Psm race 1 earlier in the season (October) compared to Pss (November). The most severe infections occurred in the dormancy period (November), while the necroses formed in January and March were less in their length. Inoculations of the two-/three-year-old branches performed in November resulted in necrosis, with those induced by Pss being more aggressive. The January inoculations, however, resulted in less formed necroses in length. Laboratory tests performed on excised sweet cherry branches confirmed the results yielded by the field experiments, indicating that the dormancy period poses the greatest risk for the P. syringae pvs. infection. The isolation of Pss and Psm bacteria from naturally infected sweet cherry samples (cankers) during the summer indicated that the bacteria that persists in cankers in low numbers became active during autumn. Significant Pss and Psm race 1 epiphytic bacterial populations were noted during the spring, but decreased in the summer and increased again in the autumn, indicating the presence of inoculum reservoirs. The greater understanding of the control strategies aimed at the epidemiological factors should, thus, facilitate better disease outbreak management.

Keywords: bacterial dieback; field and laboratory inoculation; natural infection; sweet cherry

Published: June 10, 2021  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Iličić R, Balaž J, Ognjanov V, Popović T. Epidemiology studies of Pseudomonas syringae pathovars associated with bacterial canker on the sweet cherry in Serbia. Plant Protect. Sci. 2021;57(3):196-205. doi: 10.17221/140/2020-PPS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    Supplementary files:

    Download file140-2020 Ilcic ESM.pdf

    File size: 503.03 kB

    References

    1. Arsenijević M. (1980): Further investigations on Pseudomonas syringae van Hall as pathogen of apricot in Yugoslavia. Zaštita Bilja, 152: 121-127.
    2. Balaž J., Iličić R., Ognjanov V., Ivanović Ž., Popović T. (2016): Etiology and epidemiology of bacterial canker on young sweet cherry trees in Serbia. Journal of Plant Pathology, 98: 285-294.
    3. Bereswill S., Bugert P., Volksch B., Ullrich M., Bender C., Geider K. (1994): Identification and relatedness of coronatineproducing Pseudomonas syringae pathovars by PCR analysis and sequence determination of the amplification products. Applied and Environmental Microbiology, 8: 2924-2930. Go to original source... Go to PubMed...
    4. Bultreys A., Gheysen I. (1999): Biological and molecular detection of toxic lipodepsipeptide-producing Pseudomonas syringae strains and PCR identification in plants. Applied and Environmental Microbiology, 65: 1904-1909. Go to original source... Go to PubMed...
    5. Bultreys A., Kaluzna M. (2010): Bacterial cankers caused by Pseudomonas syringae on stone fruit species with special emphasis on the pathovars syringae and morsprunorim race 1 and 2. Journal of Plant Pathology, 92: S1.21-S.1.33.
    6. Cameron H.R. (1962): Disease of deciduous fruit trees incited by Pseudomonas syringae van Hall: A review of the literature with additional data. Corvallis, Agricultural Experiment Station, Oregon State University.
    7. Cao T., Kirkpatrick B.C., Shackel K.A., De Jong T.M. (2013): Effect of leaf scar age, chilling and freezing-thawing on infection of Pseudomonas syringae pv. syringae though leaf scars and lenticels in stone fruits. Fruits, 68: 159-169. Go to original source...
    8. Crosse J.E. (1957): Bacterial canker of stone fruits. III. Inoculum concentration and the time of inoculation in relation to leaf - Scar infection of cherry. Annals of Applied Biology, 45: 19-35. Go to original source...
    9. Crosse J.E. (1959): Bacterial canker of stone fruits. IV. Investigation of a method for measuring the inoculum potential of cherry trees. Annals of Applied Biology, 47: 306-317. Go to original source...
    10. Crosse J.E. (1966): Epidemiological relations of the Pseudomonad pathogens of deciduous fruit trees. Annual Review of Phytopathology, 4: 291-310. Go to original source...
    11. Crosse J.E., Garrett, C.M.E. (1966): Bacterial canker of stonefruits. Infection experiments with Pseudomonas morsprunorum and P. syringae. Annals of Applied Biology, 58: 31-41. Go to original source...
    12. Crosse J.E., Garrett C.M.E. (1970): Pathogenicity of Pseudomonas morsprunorum in relation to host specificity. Journal of General Microbiology, 62: 315-327. Go to original source...
    13. Dye D.W. (1954): Blast of stone fruit in New Zeland. New Zeland Journal of Science and Technology, 35: 451-461.
    14. Erickson D. (1945): Certain aspects of resistance of plum trees to bacterial canker. I. Some biochemical characteristics of Pseudomonas mors-prunorum Wormald and related phytopathogenic bacteria. Annals of Applied Biology, 32: 44-52. Go to original source...
    15. Garrett C.M.E. (1979): Screening Prunus rootstocks for resistance to bacterial canker caused by Pseudomonas morsprunorum. Journal of Horticultural Science, 54: 189-194. Go to original source...
    16. Hattingh M.J., Roos I.M., Mansvelt E.L. (1989): Infection and systemic invasion of deciduous fruit trees by Pseudomonas syringae in South Africa. Plant Disease, 73: 784-789. Go to original source...
    17. Hinrichs-Berger J. (2003): Epidemiology of Pseudomonas syringae pathovars associated with decline of plum trees in the Southwest of Germany. Journal of Phytopathology, 152: 153-160. Go to original source...
    18. Hulin M.T., Mansfield J.W., Brain P., Xu X., Jackson R.W. (2018): Characterization of the pathogenicity os strains of Pseudomonas syringae towards cherry and plum. Plant Pathology, 67: 1117-1193. Go to original source... Go to PubMed...
    19. Iličić R., Balaž J., Stojšin V., Jošić D. (2016): Characterization of Pseudomonas syringae pathovars from different sweet cherry cultivars by RAPDanalysis. Genetika, 48: 285-295. Go to original source...
    20. Iličić R., Popović T., Jelušić A., Marković S., Vlajić S. (2019): New records of Pseudomonas syringae in young sweet cherry plantations. In: VIII Congress on Plant Protection. Zlatibor, Nov 25-29: 185.
    21. Kaluzna M., Willems A., Pothier F., Ruinelli M., Sobiczewski P., Pulawska J. (2016): Pseudomonas cerasi sp. nov. (non Griffin, 1911) isolated from diseased tissue of cherry. Systematic and Applied Microbiology, 39: 370-377. Go to original source... Go to PubMed...
    22. Kennelly M.M., Cazorla F.M., Vicente A., Ramos C., Sundin G.W. (2007): Pseudomonas syringae disease of fruit trees, progress toward understanding and control. Plant Disease, 91: 4-17. Go to original source... Go to PubMed...
    23. Klement Z., Rozsnyay D.S., Arsenijević M. (1974): Apoplexy of apricots. III Relationship of winter frost and the bacterial canker die-back of apricots. Acta Phytopathologica Academiae Scientiarum Hungaricae, 9: 35-45.
    24. Klement Z., Rozsnyay D.S., Báló E., Pánczél M., Prileszky G. (1984): The effect of cold on development of bacterial canker in apricot trees infected with Pseudomonas syringae pv. syringae. Physiological Plant Pathology, 24: 237-246. Go to original source...
    25. Konavko D., Moročko-Bičevska I., Bankina B. (2014): Pseudomonas syringae as important pathogen of fruit trees with emphasis on plum and cherry. Research for Rural Development, 1: 19-25.
    26. Latorre B.A., Jones A.L. (1979): Pseudomonas morsprunorum, the cause of bacterial canker of sour cherry in Michigan and its epiphytic association with P. syringae. Phytopathology, 69: 335-339. Go to original source...
    27. Lelliott R.A., Billing E., Hayward A.C. (1966): A determinative scheme for the fluorescent plant pathogenic Pseudomonads. Journal of Applied Bacteriology, 29: 470-489. Go to original source... Go to PubMed...
    28. Prunier J.P. (1977): Survival of Pseudomonas morsprunorum in canker. Report East Mailling Research Station for 1976. Maidstone, East Malling Research Station.
    29. Roos I.M.M., Hattingh M.J. (1983): Fluorescent pseudomonads associated with bacterial canker of stone fruit in South Africa. Plant Diseases, 67: 1267-1269. Go to original source...
    30. Roos I.M.M., Hattingh M.J. (1986): Resident population of Pseudomonas syringae on stone fruit tree leaves in South Africa. Phytophylactica, 18: 55-58.
    31. Scortichini M. (2010): Epidemiology and predisposing factors of some major bacterial diseases of stone and nut fruit trees species. Journal of Plant Pathology, 92: S1.73-S1.78
    32. Sobiczewski P., Jones A.L. (1992): Effect of exposure to freezing temperature on necrosis in sweet cherry shoots inoculated with Pseudomonas syringaepv. syringae of P. s. morsprunorum. Plant Disease, 76: 447-451. Go to original source...
    33. Sorensen K.N., Kim K.H., Takemoto J.Y. (1998): PCR detection of cyclic lipodepsinonapeptide - Producing Pseudomonas syringae pv. syringae and similarity strains. Applied and Environmental Microbiology, 64: 226-320. Go to original source... Go to PubMed...
    34. Spotts R.A., Wallis K.M., Serdani M., Azarenko A.N. (2010): Bacterial canker of sweet cherry in Oregon - Infection of hotricultural and natural wounds, and resistance of cultivar and rootstock combination. Plant Disease, 93: 345-350. Go to original source... Go to PubMed...
    35. Sundin G.W., Jones A.L., Olson B.D. (1988): Overwintering and population dynamics of Pseudomonas syringae pv. syringae and P.s. pv. morsprunorum on sweet and sour cherrytrees. Canadian Journal of Plant Pathology, 4: 281-288. Go to original source...
    36. Wimalajeewa D.L.S. (1987): Seasonal variation in susceptibility of apricot to Pseudomonas syringaepv. syringae (bacterial canker), and site of infection in apricot and cherry. Australian Journal of Experimental Agriculture, 27: 475-479. Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content