Plant Protect. Sci., 2022, 58(2):110-124 | DOI: 10.17221/8/2020-PPS

Genetic diversity analysis in blackgram (Vigna mungo) genotypes using microsatellite markers for resistance to Yellow mosaic virusOriginal Paper

Chippy Ayyappan Korattukudy1, Andrew Peter Leon Michael Antony Samy Tamilarasi2, Kannan Rengasamy3, Shoba Devadhasan2, Saravanan Shunmugavel2, Jeshima Khan Yasin4, Arumugam Pillai Madhavan ORCID...*,1
1 Department of Plant Breeding and Genetics, College of Agriculture, Kerala Agricultural University, Vellayani, India
2 Department of Plant Breeding and Genetics, Agricultural College and Research Institute, Killikulam, India
3 Department of Plant Pathology, Agricultural College and Research Institute, Killikulam, India
4 Division of Genomic Resources, National Bureau of Plant Genetic Resources, PUSA Campus, New Delhi, India

One hundred and two blackgram genotypes were evaluated for Yellow mosaic virus (YMV) resistance based on disease scoring, molecular characterisation, yield and morphological observation. An analysis of variance and a correlation analysis were performed on nine biometric traits. The genotypes showed the highest genetic variation for a single plant yield (genotypic coefficient variation 42.72%). The genotypes were grouped into 18 clusters based on the morphological data. The genetic divergence among the blackgram genotypes was differentiated by 60 alleles using 14 polymorphic simple sequence repeat markers. The polymorphism information content value varied from 0.37 to 0.79. The multivariate analyses of the simple sequence repeat marker scorings divided the germplasm into five divergent clusters wherein, clusters viz., II, III and V accommodated 61 genotypes and all these genotypes were resistant to moderately resistant for YMV. The tolerant accessions identified from this present investigation can be directly used for further crop improvement programmes.

Keywords: black gram germplasm; YMV resistance; polymorphic information content

Published: March 28, 2022  Show citation

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Korattukudy CA, Tamilarasi APLMAS, Rengasamy K, Devadhasan S, Shunmugavel S, Yasin JK, Madhavan AP. Genetic diversity analysis in blackgram (Vigna mungo) genotypes using microsatellite markers for resistance to Yellow mosaic virus. Plant Protect. Sci. 2022;58(2):110-124. doi: 10.17221/8/2020-PPS.
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